Der Einfluss verschiedener Volumenersatzmittel auf die Funktion von neutrophilen Granulozyten in vitro

Abstract

The physiological significance of these changes in a range of 10% has to be clarified in further investigations.

Influence of colloid fluids on polymorphonuclear granulocyte function in vivo

“…All patients were premedicated with 7.5 mg midazolam p.o., to reach a similar degree of sedation (6). The time and volume schedule was comparable to the previous in vitro study (5). In vitro , an incubation time of 30 min was sufficient to increase expression of complement receptors.…”

“…The result was a number of 15 patients for each group. This calculation was based on our in vitro study (5), in which treatment of whole blood of 10 donors with colloids revealed significant differences. The mean±standard deviation (SD) of MFI was calculated for all parameters at each data point.…”

“…In a previous investigation we could demonstrate an increase of the complement receptors CR1 (CD35) and CR3 (CD11b/CD18) on the surface of PMN after incubation of whole blood with gelatine in vitro (5). These alterations were obtained at concentrations corresponding to a 500 mL infusion in a 70‐kg man.…”

Impact of Acute Normovolemic Hemodilution in Organ and Cell Structure

“…The effects of different fluids on immune system response have been demonstrated in vitro and in vivo studies during sepsis, hemorrhagic shock and hemodilution. The activation of the inflammatory system following massive fluid resuscitation is intimately related to negative outcomes (Cotton et al, 2006;Lee et al, 2005;Rhee et al, 1998;Welters et al, 2000). Several studies have shown divergent results because different colloids may bear pro-inflammatory (Alam et al, 2004;Lee, 2005;Rhee et al, 2000) and anti-inflammatory effects (Alam et al, 2000;Jaeger et al, 2001;Lang et al, 2003).…”

Increased Levels of Serum S100B Protein in Critically Ill Patients Without Brain Injury

“…In four controlled in vitro and animal studies, HES was either proinflammatory (16,22,23) or devoid of anti-inflammatory activity displayed by albumin (21). Two studies showed no difference between HES and albumin in modulation of inflammatory processes (3,11), whereas results of one study were inconsistent (17).…”