Deregulation of miR-126 expression in colorectal cancer pathogenesis and its clinical significance

Toward the promise of microRNAs – Enhancing reproducibility and rigor in microRNA research

“…Again, because of tissue-level expression data, a miR-126 signal has repeatedly been reported as lower in cancer vs. normal tissue comparisons. 9,10 As a result, its levels are then experimentally manipulated, likely inappropriately, in epithelial cancer cell lines, in an effort to determine function. 10,11 microRNAs miR-451a and miR-144 have yet to be identified in any non-neoplastic cell type, yet they repeatedly are assigned non-RBC functionality due to tissue level data being confused for cell level expression data (Tables 1 and 2). 12-17 miR-486 is also abundant in platelets, but neither RBC nor platelet expression justifies its assigned role in a variety of disease states in which it is unlikely to participate.…”

“…9,10 As a result, its levels are then experimentally manipulated, likely inappropriately, in epithelial cancer cell lines, in an effort to determine function. 10,11 microRNAs miR-451a and miR-144 have yet to be identified in any non-neoplastic cell type, yet they repeatedly are assigned non-RBC functionality due to tissue level data being confused for cell level expression data (Tables 1 and 2). 12-17 miR-486 is also abundant in platelets, but neither RBC nor platelet expression justifies its assigned role in a variety of disease states in which it is unlikely to participate. 18,19 Thus it is important to identify the cellular source of a tissue-level microRNA signature, which can be done by checking known datasets, 5,20 obtaining cell-specific data from the Sequence Read Archive or Gene Expression Omnibus, or measuring the microRNA by qPCR or northern blot in a cell type of interest.…”

Overexpression of microRNA-1288 in oesophageal squamous cell carcinoma

“…Tissue blocks from the selected samples were sectioned and miRNA extraction was performed as previous reported [5,[8][9]. Briefly, the miRNA extraction from tissues and cell lines were performed with Qiagen miRNeasy FFPE Kits (Qiagen Pty.…”

“…Expression levels of miR-1288 were analysed as in our previous study [5,[8][9]. Fold change of miR-1288 expression was calculated using 2 -[delta][delta]Ct method by comparing the expression levels in the control tissues /cells.…”

“…Oesophageal cancer has been reported to be the ninth most common malignancy worldwide and is composed of different histological subtypes with diverse cellular and molecular bases [1][2][3][4]. Recent studies have proven that small non coding RNAs known as microRNAs (miRNAs) play a significant role in the regulation of gene expressions in cancer cell differentiation, cell cycle progression, apoptosis, migration and metastasis [5][6][7][8][9].…”