Deregulation of Serum MicroRNA Expression Is Associated with Cigarette Smoking and Lung Cancer

Abstract

Lung cancer is the leading cause of cancer-related death and cigarette smoking is the main risk factor for lung cancer. Circulating microRNAs (miRNAs) are considered potential biomarkers of various cancers, including lung cancer. However, it is unclear whether changes in circulating miRNAs are associated with smoking and smoking-related lung cancer. In this study, we determined the serum miRNA profiles of 10 nonsmokers, 10 smokers, and 10 lung-cancer patients with miRCURY LNA microRNA arrays. The differentially expressed miRNAs were then confirmed in a larger sample. We found that let-7i-3p and miR-154-5p were significantly downregulated in the sera of smokers and lung-cancer patients, so the serum levels of let-7i-3p and miR-154-5p are associated with smoking and smoking-related lung cancer. The areas under receiver operating characteristic curves for let-7i-3p and miR-154-5p were approximately 0.892 and 0.957, respectively. In conclusion, our results indicate that changes in serum miRNAs are associated with cigarette smoking and lung cancer and that let-7i-3p and miR-154-5p are potential biomarkers of smoking-related lung cancer.

Serum exosomal microRNA let‐7i‐3p as candidate diagnostic biomarker for Kawasaki disease patients with coronary artery aneurysm

“…Indersie et al reported that let‐71‐3p, miR‐449b‐3p, and miR‐885‐5p were decreased in tumors compared to normal livers, and that these miRNAs inhibited HBL cell grow and Wnt signaling activity in vitro, partly through beta‐catenin downregulation . Some studies predicted the potential target genes of let‐7i‐3p and its involvement in various cellular pathways . It was shown that these target genes such as CXCR6 and CXCL12, which play roles in leukocyte transmigration, are key molecule candidates in KD .…”

Differential micro ribonucleic acid expression profiling in ovarian endometrioma with leuprolide acetate treatment

“…Circulating levels of miR‐939 were used to differentiate between cirrhotic patients with or without hepatocellular carcinoma . MiR‐154 was reduced in the serum of smokers and lung cancer patients relative to non‐smoking healthy individuals . Serum miR‐142‐3p was reduced in patients with colorectal carcinoma .…”

“…59 MiR-154 was reduced in the serum of smokers and lung cancer patients relative to non-smoking healthy individuals. 60 Serum miR-142-3p was reduced in patients with colorectal carcinoma. 61 These observations suggest that the detection of a combination of multiple miRNAs is needed to improve the detection and clinical outcomes of endometriosis in relation to other diseases.…”

Circulating microRNAs and diabetes: potential applications in medical practice

“…Several studies have reported that acute physical activity and prolonged exercise training programmes can modify the circulating miRNA profile [87]. Furthermore, smoking appears to alter the pattern of circulating miRNAs [88][89][90][91], and of the miRNAs affected, miR-29b was identified to be deregulated in diabetic patients [27,28,70]. Environmental exposure to air pollutants and chemicals seems to modify circulating miRNAs [92], inducing a bias that is difficult to estimate.…”