Derepression of the synthesis of D-ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum

Sarles, Landry; Tabita, F. Robert

doi:10.1128/jb.153.1.458-464.1983

Cited by 49 publications

(15 citation statements)

References 14 publications

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“…1). R. rubrum compensates for the low affinity of its form II RubisCO by massively overexpressing this enzyme, to 50% of total soluble protein, when grown autotrophically under low-CO 2 conditions (Sarles and Tabita 1983). The amount of RubisCO detected here suggests that the symbionts have a high concentration of intracellular CO 2 .…”

Section: Discussionmentioning

confidence: 65%

Kinetic isotope effect and characterization of form II RubisCO from the chemoautotrophic endosymbionts of the hydrothermal vent tubeworm Riftia pachyptila

Robinson

Scott

Swanson

et al. 2003

Limnology & Oceanography

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It has been hypothesized that the ␦ 13 C values of chemoautotrophs at deep-sea hydrothermal vents, which cluster in two groups around Ϫ11‰ and Ϫ30‰, are due to variation in isotopic discrimination by different forms of RubisCO. The most enriched ␦ 13 C values are from the vestimentiferan tubeworm Riftia pachyptila, whose bacterial endosymbionts provide essentially all of its organic carbon via CO 2 fixation by a form II RubisCO. The kinetic parameters of purified R. pachyptila symbiont RubisCO were determined to assess the degree to which the ␦ 13 C values of tubeworm biomass are due to isotopic fractionation during CO 2 fixation by this enzyme. Like most form II enzymes, the K is high, at 240 mol L Ϫ1 , whereas the CO 2 /O 2 specificity factor (⍀) is low, at 8.6. The CO2 value, which is proportional to the degree of isotopic discrimination by the enzyme, was determined to be 19.5‰, lower than that for form I RubisCO. This low value supports the hypothesis that the degree of isotopic fractionation during CO 2 fixation appears to be an important influence on the ␦ 13C values of R. pachyptila biomass. Our results indicate that it is necessary to consider RubisCO values in interpreting ␦ 13 C values from autotrophs.At deep-sea hydrothermal vents, invertebrates with chemoautotrophic bacterial symbionts provide a substantial por-1 Present address: E&J Gallo Winery,

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Section: Discussionmentioning

confidence: 65%

Kinetic isotope effect and characterization of form II RubisCO from the chemoautotrophic endosymbionts of the hydrothermal vent tubeworm Riftia pachyptila

Robinson

Scott

Swanson

et al. 2003

Limnology & Oceanography

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“…It has been previously shown that members of the Rhodospirillaceae derepress RuBP carboxylase/oxygenase synthesis to high levels during the stationary phase when grown with butyrate as the electron donor (15,17). This appears to be due, at least in part, -to the depletion of carbon dioxide from the medium (14). In addition, when wild-type R. sphaeroides was grown on malate and transferred, after extensive washing, to a medium containing no exogenous carbon, RuBP carboxylase/oxygenase levels were increased substantially.…”

Section: Resultsmentioning

confidence: 99%

Isolation and partial characterization of Rhodopseudomonas sphaeroides mutants defective in the regulation of ribulose bisphosphate carboxylase/oxygenase

Weaver¹,

Tabita²

1983

J Bacteriol

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Several mutants of Rhodopseudomonas sphaeroides defective in the derepression of the enzyme ribulose 1,5-bisphosphate carboxylase have been isolated by using the unstable Tn5 vectors pJB4JI and pRK340. Transpositional insertion mutants obtained with pJB4JI were demonstrated to be incapable of increasing ribulose 1,5-bisphosphate carboxylase/oxygenase levels when grown on butyrate-bicarbonate medium or under conditions of carbon starvation, whereas the wild-type strain increased activity four- to eightfold. When the wild-type strain was starved for carbon in the presence of chloramphenicol, no derepression was observed. Crude extracts from mutant and wild-type strains had distinct and consistent differences in protein content as observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Chromatographic evidence indicated that mutants were defective in the regulation of only one of the two forms of ribulose 1,5-bisphosphate carboxylase/oxygenase synthesized by R. sphaeroides.

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“…Growth of R. rubrum in the butyrate-containing medium used in the present studies induces the biosynthesis of rubisco (34,40). The present research establishes that the poly(A)+ RNA species contain mRNA corresponding to this enzyme.…”

Section: Discussionmentioning

confidence: 88%

“…In Rhodospirillum rubrum grown in the light on butyrate-bicarbonate medium this enzyme comprises ca. 7% of the soluble protein (40), and maximal levels of rubisco are found after 5 to 6 days of growth under these conditions (34,39). In this communication, we MATERIALS AND METHODS Chemicals.…”

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confidence: 99%

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Majumdar¹,

McFadden²

1984

J Bacteriol

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Total cellular RNA extracted fromh Rhodospirillum rubrum cultured in butyrate-containing medium under strict photosynthetic conditions to the stationary phase of growth has been fractionated on an oligodeoxythymidylic acid-cellulose column into polyadenylated [poly(A)'] RNA and poly(A)-RNA fractions. The poly(A)+ fraction was 9 to 10% of the total bulk RNA isolated. Atnalysis of the poly(A)+ RNA on a denaturing urea-polyacrylamide gel revealed four sharp bands of RNA distributed in heterodisperse fashion between 16S and 9S. Similar fractionation of the poly(A)-RNA resulted in the separation of 23, 16, and 5S rRNAs and 4S tRNA. Poly(A)+ fragments isolated after combined digestion with pancreatic A and T1 RNases and analysis by denaturing gel electrophoresis demonstrated two major components of 80 and 100 residues. Alkaline hydrolysis of the nuclease-resistant, purified residues showed AMP-rich nucleotides. Through the use of snake venom phosphodiesterase, poly(A) tracts were placed at the 3' end of poly(A)+ RNA. Stimtlation of [3H]leucine incorporation into hot trichloroacetic acid-precipitable polypeptides in a cell-free system from wheat germ primed by the poly(A)+ RNA mixture was found to be 220-fold higher than that for poly(A)-RNAs (on a unit mass basis), a finding which demonstrated that poly(A)+ RNAs in R. rubrum are mRNAs. Gel electrophoretic analysis of the translation mixture revealed numerous 3H-labeled products including a major band (Mr, 52,000). The parent protein was precipitated by antibodies to ribulose bisphosphate carboxylase-oxygenase and comprised 6.5% of the total translation products.

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Derepression of the synthesis of D-ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum

Cited by 49 publications

References 14 publications

Kinetic isotope effect and characterization of form II RubisCO from the chemoautotrophic endosymbionts of the hydrothermal vent tubeworm Riftia pachyptila

Kinetic isotope effect and characterization of form II RubisCO from the chemoautotrophic endosymbionts of the hydrothermal vent tubeworm Riftia pachyptila

Isolation and partial characterization of Rhodopseudomonas sphaeroides mutants defective in the regulation of ribulose bisphosphate carboxylase/oxygenase

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

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