Derivation of a human blastocyst after heterologous nuclear transfer to donated oocytes

Stojković, Miodrag; Stojković, Petra; Leary, Christine; Hall, Vanessa Jane; Armstrong, Lyle; Herbert, Mary; Nesbitt, M; Lako, Majlinda; Murdoch, Alison

doi:10.1016/s1472-6483(10)60962-5

Cited by 140 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although a single group has reported efficient transcriptional reprogramming after human nuclear transfer 20 , they compared somatic cells to nuclear transfer samples and their results are therefore confounded by the presence of maternal mRNAs, which were not appropriately accounted for by their analyses. Another group has generated a single blastocyst after transfer of embryonic stem cell nuclei into human oocytes 17 , but development arrested when fibroblasts were transferred using identical methods 18 . This suggests that development and activation of the transferred genome depend on the epigenetic state of the injected nucleus.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Reprogramming within hours following nuclear transfer into mouse but not human zygotes

et al. 2011

View full text Add to dashboard Cite

Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes might therefore be useful for producing patient-derived pluripotent stem cells. However, logistical, legal and social considerations have limited the availability of human eggs for research. Here we show that a significant number of normal fertilized eggs (zygotes) can be obtained for reprogramming studies. Using these zygotes, we found that when the zygotic genome was replaced with that of a somatic cell, development progressed normally throughout the cleavage stages, but then arrested before the morula stage. This arrest was associated with a failure to activate transcription in the transferred somatic genome. In contrast to human zygotes, mouse zygotes reprogrammed the somatic cell genome to a pluripotent state within hours after transfer. Our results suggest that there is a previously unappreciated barrier to successful human nuclear transfer, and that future studies should focus on the requirements for genome activation.

show abstract

Section: Discussionmentioning

confidence: 99%

“…However, despite several attempts at human nuclear transfer 17-24 , these efforts have uniformly failed to produce stem cell lines. Instead, most studies reported developmental arrest during the cleavage stages.…”

Section: Introductionmentioning

confidence: 99%

Reprogramming within hours following nuclear transfer into mouse but not human zygotes

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Murdoch's position at the head of the pack is ironic because, when her team published its results in June of last year (Stojkovic, et al, 2005), it looked like an also-ran. The month before, Hwang and colleagues had announced fantastic success with SCNT, claiming not only to have created human blastocysts, but to have turned them into 11 ESC lines.…”

Section: Mergers and Acquisitionsmentioning

confidence: 99%

The action behind the words: embryonic stem cell research marches on

Leslie¹

2006

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…The enforced expression of specific combinations of transcription factors can override and modulate existing gene networks and epigenetic marks. Indeed, an ability to induce pluripotency in somatic cells was previously demonstrated in elegant studies of the transfer of somatic cell nuclei into enucleated oocytes and the fusion of pluripotent stem cells with differentiated cells (5, 6). …”

Section: Introductionmentioning

confidence: 97%

Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction

Nethercott

Brick

Schwartz

2011

Methods in Molecular Biology

View full text Add to dashboard Cite

This chapter provides a method for reprogramming human dermal fibroblasts into induced pluripotent stem cells (iPSCs) using three lentiviruses containing cDNAs for OCT4 and SOX2, KLF4 and C-MYC, and NANOG and LIN28, respectively. Lentiviral vectors are based on the human immunodeficiency virus (HIV) and provide an effective means for the delivery, integration, and expression of exogenous genes in mammalian cells. Lentiviruses are attractive gene delivery vehicles as they are able to infect both proliferating and nonproliferating cells. Lentiviruses stably integrate into the genome without incurring cellular toxicity and can maintain sustained transgene expression during prolonged host cell proliferation and differentiation. In this protocol, we describe how to prepare lentiviruses, stably transduce human fibroblasts, and identify bona fide iPSC colonies based on morphological similarity to human embryonic stem cell (ESC) colonies and live-cell immunological staining using cell-surface markers of human PSCs such as Tra-1-60 and Tra-1-81.

show abstract

Derivation of a human blastocyst after heterologous nuclear transfer to donated oocytes

Cited by 140 publications

References 29 publications

Reprogramming within hours following nuclear transfer into mouse but not human zygotes

Reprogramming within hours following nuclear transfer into mouse but not human zygotes

The action behind the words: embryonic stem cell research marches on

Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction

Contact Info

Product

Resources

About