Derivation of pancreatic acinar cell carcinoma cell line HS‐1 as a patient‐derived tumor organoid

Abstract

Acinar cell carcinoma (ACC) of the pancreas is a malignant tumor of the exocrine cell lineage with a poor prognosis. Due to its rare incidence and technical difficulties, few authentic human cell lines are currently available, hampering detailed investigations of ACC. Therefore, we applied the organoid culture technique to various types of specimens, such as bile, biopsy, and resected tumor, obtained from a single ACC patient.Despite the initial propagation, none of these organoids achieved long-term proliferation or tolerated cryopreservation, confirming the challenging nature of establishing ACC cell lines. Nevertheless, the biopsy-derived early passage organoid developed subcutaneous tumors in immunodeficient mice. The xenograft tumor histologically resembled the original tumor and gave rise to infinitely propagating organoids with solid features and high levels of trypsin secretion. Moreover, the organoid stained positive for carboxylic ester hydrolase, a specific ACC marker, but negative for the duct cell marker CD133 and the endocrine lineage marker synaptophysin. Hence, we concluded the derivation of a novel ACC cell line of the pure exocrine lineage,

A quick and reliable image-based AI algorithm for evaluating cellular senescence of gastric organoids

“…Cells of cancer organoids may acquire capabilities to sustain proliferative signaling, evade growth suppressors, resist cell death, and enable replicative immortality. Recently, this infinite proliferation potential has been proposed to support the utility of PDOs in establishing cell lines to serve as experimental models 44 , 45 .…”

Roseate and merest-acinar cell carcinoma-pancreas

“…Additionally, recurrent genetic rearrangements within BRAF, RAF1 and RET genes may be discerned. 3,4 A subset of neoplasms are associated with modifications within Wnt signalling pathway in association with APC / CTNNB1 genetic mutations. Majority of neoplasms arise sporadically.…”

“…However, a minority (< 10%) of tumours are associated with conditions such as Lynch syndrome, familial adenomatous polyposis syndrome or Carney complex. 3,4 Acinar cell carcinoma pancreas manifests cogent clinical symptoms as abdominal pain, dorso-lumbar pain, loss of weight, nausea or vomiting. Hyperbilirubinemia is exceptional, in contrast to ductal adenocarcinoma.…”

“…1,2 Acinar cell carcinoma pancreas is posited to occur due to cellular accumulation of genetic alterations or occurrence of chromosomal instability a Acinar cell carcinoma nd frequent allelic copy number variation. 3,4 Additionally, acinar cell carcinoma is postulated to arise from factors such as tobacco consumption or cigarette smoking and defective repair of deoxy ribonucleic acid (DNA) (Table 1).…”

Comprehensive review of pancreatic acinar cell carcinoma: epidemiology, diagnosis, molecular features and treatment

“…PDOs are useful for determining the sensitivity to chemotherapy and the preclinical evaluation of novel therapies in patients with pancreatic cancer ( 81–84 ). Recently, a PACC cell line was established using PDOs ( 85 ). The development of PDOs from patients with PACC could help provide new insights into the biological characteristics of PACC and useful data for personalized medicine and drug discovery.…”