Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms

Abstract

A derivative spectrophotometric method was proposed for the simultaneous determination of clindamycin and tretinoin in pharmaceutical dosage forms. The measurement was achieved using the first and second derivative signals of clindamycin at (1D) 251 nm and (2D) 239 nm and tretinoin at (1D) 364 nm and (2D) 387 nm.The proposed method showed excellent linearity at both first and second derivative order in the range of 60–1200 and 1.25–25 μg/ml for clindamycin phosphate and tretinoin respectively. The within-day and between-day precision and accuracy was in acceptable range (CV<3.81%, error<3.20%). Good agreement between the found and added concentrations indicates successful application of the proposed method for simultaneous determination of clindamycin and tretinoin in synthetic mixtures and pharmaceutical dosage form.

The application of quantitative NMR for the facile, rapid and reliable determination of clindamycin phosphate in a conventional tablet formulation

“…The water‐soluble semi‐synthetic lincosamide antibiotic, clindamycin phosphate (CLP), is primarily used to treat a broad range of anaerobic infections, with its spectrum of activity extending to staphylococcus, streptococcus and pneumococcus. There exist a handful of reports in the literature on analytical techniques, which have been successfully applied toward the determination of CLP in primarily liquid dosage forms, which include HPLC, derivative spectrophotometric methods and kinetic spectrophotometric methods, while little has been reported on the use of quantitative NMR (qNMR) for this purpose . Previously reported approaches are also hampered by the necessity of requiring an internal standard being near identical to the target analyte, while each is also quite laborious and require relatively greater amounts of analyte relative to qNMR.…”

“…The theory of qNMR is based on the premise that peak integrations are directly proportional to the number of resonant nuclei involved in generating the signal . Accordingly, qNMR can then be used to calculate the amount of target analyte in a given sample from ratio of peak intensities between the target compound and an internal standard compound.…”

Rapid and efficient colorimetric sensing of clindamycin and Fe3+ using controllable phyto-synthesized silver/silver chloride nanoparticles by Syzygium cumini fruit extract

“…The lack of an absorbing peak in the UV-Vis region is the main problem in the spectrophotometric determination of clindamycin. In the previous reports, a derivative reaction for the conversion of clindamycin into a UV-Vis active compound was used to measure its concentration spectrophotometrically (Barazandeh Tehrani et al 2013;El-Yazbi and Blaih 1993), which is not generally an easy and reproducible procedure. So, to quantify the clindamycin in the pharmaceutical dosage form, the development of direct spectrophotometric methods is required (El-Yazbi and Blaih 1993).…”

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“…It is chemically 6-[3-(adamantan-1-yl)-4-methoxyphenyl] naphthalene-2-Carboxylic acid. Literature survey [5][6][7][8][9][10] reveals that few spectrophotometric and chromatographic methods were reported for estimation of CLIN and ADA in single and combination with other drugs. In this study, an attempt has been made to develop an accurate, rapid and reproducible reverse phase HPLC method for simultaneous determination of CLIN and ADA in tablet dosage…”