Derivatization of fluorinated polymers and their potential use for the construction of biosensors

Glodek, Janina; Milka, Peter; Krest, Ingo; Keusgen, Michael

doi:10.1016/s0925-4005(01)01048-6

Cited by 17 publications

(8 citation statements)

References 13 publications

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“…These enhancements like that, easy process ability, excellent barrier behavior, surface finishing versatility, balanced properties. Modifications of these properties allow to extensive use in different applications such as, packaging, aircraft industry, electronic equipment, health care [1,2], orthopedic science [3], tissue engineering [4], immobilization of lipase [5][6][7][8], cell culture and drug delivery [9], etc.. These applications need to enhance the surface properties of the irradiated polymer, such as optical, mechanical, wettability, surface free energy, roughness and adhesion properties [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

The optical, wettability and hardness properties of polyethylene improved by alpha particle irradiations

Zaki

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Section: Introductionmentioning

confidence: 99%

The optical, wettability and hardness properties of polyethylene improved by alpha particle irradiations

Zaki

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…a Immobilization of biomaterials by adsorption onto a surface of a solid support, b integration of biomaterials into a gel structure, c covalent coupling of enzymes, antibodies, sugars and cells to a solid support done under physiological conditions. Alternatively, epoxides, benzoquinone, and 2-aminochloro-4,6-dichloros-triazine are suitable coupling reagents that allow immobilization of a wide variety of bio-components suitable for the construction of sensors (Keusgen 1999;Milka et al 2000;Keusgen et al 2001a;Glodek et al 2002).…”

Section: Immobilization Techniquesmentioning

confidence: 99%

“…Thus, the outer end of the spacer structure is activated in such a way as to allow covalent coupling of the bio-component under physiological conditions. Immobilization techniques following this route with inorganic supports are described by Milka et al (2000), and with polymer materials, especially fluorinated membranes, by Keusgen (1999), Keusgen et al (2001b), and Glodek et al (2002).…”

Section: Immobilization Techniquesmentioning

confidence: 99%

Biosensors: new approaches in drug discovery

Keusgen

2002

Naturwissenschaften

Self Cite

120

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The development of biosensors for analytical purposes has attracted a great deal of attention in recent years. A biosensor is defined as an analytical device consisting of a biological component (e.g., enzyme, antibody, entire cell, DNA) and a physical transducer (e.g., electrode, optical device). Biosensors are mostly designed for routine analysis, such as clinical diagnosis, quality control of food, in-process control of fermentations, and in environmental analysis. Many of these sensors are also suitable for screening purposes in order to find new drugs. Such systems should yield information either about compounds with known bioactivity or about the bioactivity of samples with known or unknown chemical composition. Biosensors intended for the latter purpose are essentially based on whole cells carrying receptors and ion channels at their surfaces. Miniaturization of structures, primarily based on silicon, allows integration of many sensors into arrays, which may be suitable for the screening of natural and chemical products as well as combinatorial libraries. Until now, no commercially available sensors of this kind exist but they are expected in the near future. Different biosensors, based on enzymes, antibodies, cells, artificial membranes and entire animal tissues, which can be used in drug discovery and may lead to efficient screening systems in the future, are described in this review.

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“…Functional enzymes have been successfully immobilised onto fluoropolymer supports. [20][21][22][23] The surface ion treatment of fluoropolymer films allowed patterned immobilisation of poly(acrylic acid) onto FEP films by introducing functional groups for bioconjugation. 24 In other microfluidic devices made from other materials a broad spectrum of crosslinking chemistries have been explored, 25 including indirect immobilisation via a surface polymer coating to introduce multiple reactive functional groups thereby increasing the effective antibody density and/or orientation.…”

Section: Introductionmentioning

confidence: 99%

Covalent immobilisation of antibodies in Teflon-FEP microfluidic devices for the sensitive quantification of clinically relevant protein biomarkers

Pivetal

Pereira²,

Barbosa

et al. 2017

Analyst

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This study reports for the first time the sensitive colorimetric and fluorescence detection of clinically relevant protein biomarkers by sandwich immunoassays using the covalent immobilisation of antibodies onto the fluoropolymer surface inside Teflon®-FEP microfluidic devices. Teflon®-FEP has outstanding optical transparency ideal for high-sensitivity colorimetric and fluorescence bioassays, however this thermoplastic is regarded as chemically inert and very hydrophobic. Covalent immobilisation can offer benefits over passive adsorption to plastic surfaces by allowing better control over antibody density, orientation and analyte binding capacity, and so we tested a range of different and novel covalent immobilisation strategies. We first functionalised the inner surface of a 10-bore, 200 μm internal diameter FEP microcapillary film with high-molecular weight polyvinyl alcohol (PVOH) without changing the outstanding optical transparency of the device delivered by the matched refractive index of FEP and water. Glutaraldehyde immobilisation was compared with the use of photoactivated linkers and NHS-ester crosslinkers for covalently immobilising capture antibodies onto PVOH. Three clinically relevant sandwich ELISAs were tested against the cytokine IL-1β, the myocardial infarct marker cardiac troponin I (cTnI), and the chronic heart failure marker brain natriuretic peptide (BNP). Overall, glutaraldehyde immobilisation was effective for BNP assays, but yielded unacceptable background for IL-1β and cTnI assays caused by direct binding of the biotinylated detection antibody to the modified PVOH surface. We found NHS-ester groups reacted with APTES-treated PVOH coated fluoropolymers. This facilitated a novel method for capture antibody immobilisation onto fluoropolymer devices using a bifunctional NHS-maleimide crosslinker. The density of covalently immobilised capture antibodies achieved using PVOH/APTES/NHS/maleimide approached levels seen with passive adsorption, and sensitive and quantitative assay performance was achieved using this method. Overall, the PVOH coating provided an excellent surface for controlled covalent antibody immobilisation onto Teflon®-FEP for performing high-sensitivity immunoassays.

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Derivatization of fluorinated polymers and their potential use for the construction of biosensors

Cited by 17 publications

References 13 publications

The optical, wettability and hardness properties of polyethylene improved by alpha particle irradiations

The optical, wettability and hardness properties of polyethylene improved by alpha particle irradiations

Biosensors: new approaches in drug discovery

Covalent immobilisation of antibodies in Teflon-FEP microfluidic devices for the sensitive quantification of clinically relevant protein biomarkers

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