Dermal Fibroblast Cells: Biology and Function in Skin Regeneration

Nilforoushzadeh, Mohammad Ali; Ashtiani, Hamid Reza Ahmadi; Jaffary, Fariba; Jahangiri, Faeze; Nikkhah, Nahid; Mahmoudbeyk, Mona; Fard, Maryam; Ansari, ZibaJaber; Zare, Sona

doi:10.5812/jssc.69080

Cited by 22 publications

(20 citation statements)

References 18 publications

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“…HMSCs were used as a model of mesenchymal (stem) cells present in the innermost layers of the skin, such as dermis and hypodermis (where they originate fibroblasts, among other cell types), as well as connective tissue in general [24], in order to assess the cytocompatibility and safety. Starting from the optimal concentrations chosen for HaCaT cells, each component was diluted at higher and lower concentrations to investigate the sensitivity of hMSCs to the same component.…”

Section: Discussionmentioning

confidence: 99%

Chitin Nanofibrils and Nanolignin as Functional Agents in Skin Regeneration

Danti

Trombi

Fusco

et al. 2019

IJMS

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Chitin and lignin, by-products of fishery and plant biomass, can be converted to innovative high value bio- and eco-compatible materials. On the nanoscale, high antibacterial, anti-inflammatory, cicatrizing and anti-aging activity is obtained by controlling their crystalline structure and purity. Moreover, electropositive chitin nanofibrlis (CN) can be combined with electronegative nanolignin (NL) leading to microcapsule-like systems suitable for entrapping both hydrophilic and lipophilic molecules. The aim of this study was to provide morphological, physico-chemical, thermogravimetric and biological characterization of CN, NL, and CN-NL complexes, which were also loaded with glycyrrhetinic acid (GA) as a model of a bioactive molecule. CN-NL and CN-NL/GA were thermally stable up to 114 °C and 127 °C, respectively. The compounds were administered to in vitro cultures of human keratinocytes (HaCaT cells) and human mesenchymal stromal cells (hMSCs) for potential use in skin contact applications. Cell viability, cytokine expression and effects on hMSC multipotency were studied. For each component, CN, NL, CN-NL and CN-NL/GA, non-toxic concentrations towards HaCaT cells were identified. In the keratinocyte model, the proinflammatory cytokines IL-1α, IL-1 β, IL-6, IL-8 and TNF-α that resulted were downregulated, whereas the antimicrobial peptide human β defensin-2 was upregulated by CN-LN. The hMSCs were viable, and the use of these complexes did not modify the osteo-differentiation capability of these cells. The obtained findings demonstrate that these biocomponents are cytocompatible, show anti-inflammatory activity and may serve for the delivery of biomolecules for skin care and regeneration.

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Section: Discussionmentioning

confidence: 99%

Chitin Nanofibrils and Nanolignin as Functional Agents in Skin Regeneration

Danti

Trombi

Fusco

et al. 2019

IJMS

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“…ASCs and DFs share common characteristics (such as immunophenotypic profile and differentiative potential) and, when implanted at the damaged site, both of them are able to exert immunomodulatory and regenerative actions (Nilforoushzadeh et al, 2017;Ichim et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Raman Fingerprint of Extracellular Vesicles and Conditioned Media for the Reproducibility Assessment of Cell-Free Therapeutics

Carlomagno

Giannasi

Niada

et al. 2021

Front. Bioeng. Biotechnol.

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Extracellular Vesicles (EVs) and Conditioned Medium (CM) are promising cell-free approaches to repair damaged and diseased tissues for regenerative rehabilitation purposes. They both entail several advantages, mostly in terms of safety and handling, compared to the cell-based treatment. Despite the growing interest in both EVs and CM preparations, in the light of a clinical translation, a number of aspects still need to be addressed mainly because of limits in the reproducibility and reliability of the proposed protocols. Raman spectroscopy (RS) is a non-destructive vibrational investigation method that provides detailed information about the biochemical composition of a sample, with reported ability in bulk characterization of clusters of EVs from different cell types. In the present brief report, we acquired and compared the Raman spectra of the two most promising cell-free therapeutics, i.e., EVs and CM, derived from two cytotypes with a history in the field of regenerative medicine, adipose-derived mesenchymal stem/stromal cells (ASCs) and dermal fibroblasts (DFs). Our results show how RS can verify the reproducibility not only of EV isolation, but also of the whole CM, thus accounting for both the soluble and the vesicular components of cell secretion. RS can provide hints for the identification of the soluble factors that synergistically cooperate with EVs in the regenerative effect of CM. Still, we believe that the application of RS in the pipeline of cell-free products preparation for therapeutic purposes could help in accelerating translation to clinics and regulatory approval.

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“…Fibroblasts are the main cells from the papillary dermis, lying directly under the epidermis. They have the principal function of maintaining the integrity of ECM (extracellular matrix) and its regulation, through the synthesis of type I collagen but also proteoglycans and glycosaminoglycans [22,23,24]. Fibroblasts have the capacity to regenerate the skin and are involved in skin aging (including extrinsic and intrinsic) [25,26].…”

Section: Introductionmentioning

confidence: 99%

Production of Active Poly- and Oligosaccharidic Fractions from Ulva sp. by Combining Enzyme-Assisted Extraction (EAE) and Depolymerization

et al. 2019

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Data on fractionation and depolymerization of the matrix ulvan polysaccharides, and studies on the biological activities on skin cells, are very scarce. In this work, crude ulvans were produced by using EAE (enzyme-assisted extraction) and compared to maceration (an established procedure). After different fractionation procedures—ethanolic precipitation, dialysis, or ammonium sulfate precipitation—the biochemical composition showed that EAE led to an increased content in ulvans. Coupling EAE to sulfate ammonium precipitation led to protein enrichment. Oligosaccharides were obtained by using radical depolymerization by H2O2 and ion-exchange resin depolymerization. Sulfate groups were partially cleaved during these chemical treatments. The potential bioactivity of the fractions was assessed using a lipoxygenase inhibition assay for anti-inflammatory activity and a WST-1 assay for human dermal fibroblast viability and proliferation. All ulvans extracts, poly- and oligosaccharidic fractions from EAE, expanded the fibroblast proliferation rate up to 62%. Our research emphasizes the potential use of poly- and oligosaccharidic fractions of Ulva sp. for further development in cosmetic applications.

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Dermal Fibroblast Cells: Biology and Function in Skin Regeneration

Cited by 22 publications

References 18 publications

Chitin Nanofibrils and Nanolignin as Functional Agents in Skin Regeneration

Chitin Nanofibrils and Nanolignin as Functional Agents in Skin Regeneration

Raman Fingerprint of Extracellular Vesicles and Conditioned Media for the Reproducibility Assessment of Cell-Free Therapeutics

Production of Active Poly- and Oligosaccharidic Fractions from Ulva sp. by Combining Enzyme-Assisted Extraction (EAE) and Depolymerization

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