Desalted Duck Egg White Peptides Promote Calcium Uptake and Modulate Bone Formation in the Retinoic Acid-Induced Bone Loss Rat and Caco-2 Cell Model

Abstract

Desalted duck egg white peptides (DPs) have been proven to promote calcium uptake in Caco-2 cells and rats treated with a calcium-deficient diet. The retinoic acid-induced bone loss model was used to evaluate the effect of DPs on calcium absorption and bone formation. Three-month-old Wistar female rats were treated with 0.9% saline, DPs (800 mg/kg), or alendronate (5 mg/kg) for three weeks immediately after retinoic acid treatment (80 mg/kg) once daily for two weeks. The model group was significantly higher in serum bone alkaline phosphatase than the other three groups (p < 0.05), but lower in calcium absorption rate, serum osteocalcin, bone weight index, bone calcium content, bone mineral density, and bone max load. After treatment with DPs or alendronate, the absorption rate increased and some serum and bone indices recovered. The morphology results indicated bone tissue form were ameliorated and numbers of osteoclasts decreased after supplementation with DPs or alendronate. The in vitro study showed that the transient receptor potential vanilloid 6 (TRPV6) calcium channel was the main transport pathway of both DPs and Val-Ser-Glu-Glu peptitde (VSEE), which was identified from DPs. Our results indicated that DPs could be a promising alternative to current therapeutic agents for bone loss because of the promotion of calcium uptake and regulation of bone formation.

Konjac Oligosaccharides Modulate the Gut Environment and Promote Bone Health in Calcium-Deficient Mice

“…The breaking strength was determined with a three-point bending test using a texture analyzer system TA.XTPlus (Stable Micro System, Surrey, England). 24 The femur sample was placed on two vertically parallel supports (spacing, 8 mm) with the larger side on top. Then, a load was applied to the midshaft of the femur with a constant deformation rate of 5 mm/min until a fracture occurred.…”

Desalted duck egg white peptides promoted osteogenesis via wnt/β‐catenin signal pathway

“…To improve the utilization of salted duck egg white, desalted duck egg white peptides (DPs) were prepared by electrodialysis and enzymatic hydrolysis. DPs were proved to effectively promote calcium absorption and bone strength (Zhao et al., 2014), which may be attributed to opening the transient receptor potential vanilloid 6 (TRPV6) calcium channel (Hou, Liu, Kolba, Guo, & He, 2017). DPs could also improve the calcium absorption in the presence of phytic acid by regulating enterocytes differentiation and avoid calcium precipitate in gut (Hou, Liu, Shi, Ma, & He, 2017).…”

Functional Calcium Binding Peptides from Pacific Cod (Gadus macrocephalus) Bone: Calcium Bioavailability Enhancing Activity and Anti-Osteoporosis Effects in the Ovariectomy-Induced Osteoporosis Rat Model

“…Calcium binding peptides could interact with Ca 2+ during the formation of the peptide-calcium complex [ 8 ], which was believed to be a suitable candidate for calcium supplementation [ 9 ]. In the recent decade, calcium binding peptides and the peptide-calcium complex have attracted great attention and many food proteins have been explored to prepare and isolate calcium binding peptides, including tilapia protein [ 10 ], Antarctic krill protein [ 11 ], Pacific cod skin protein [ 12 ], whey protein [ 13 ], duck egg white [ 14 ], etc. Furthermore, previous studies reported that fishbone derived peptides also possess high calcium binding activity and can improve calcium bioavailability [ 15 , 16 ].…”