Description of a medium for isolating Anaerobiospirillum spp., a possible cause of zoonotic disease, from diarrheal feces and blood of humans and use of the medium in a survey of human, canine, and feline feces

Abstract

Anaerobiospirillum spp., motile, spiral anaerobic bacteria, have been implicated as a cause of diarrhea and bacteremia in humans. Anaerobiospirillumlike organisms and Anaerobiospirillum succiniciproducens were reported from 17 cases of diarrhea. Sixteen of the patients did not have any underlying disorders and recovered from the infection; the other one, who had a heart defect, did not. The formulation of a selective medium for Anaerobiospirillum spp. has enabled a survey of human, cat, and dog feces as possible sources for these anaerobic bacteria. Anaerobiospirillum spp. were not isolated from 527 "normal" human feces but were found in both cat and dog feces. We also describe biochemical tests and API ZYM results ofA. succiniciproducens and anaerobiospirillumlike organisms.

Genetic diversity of Helicobacter pylori indexed with respect to clinical symptomatology, using a 16S rRNA and a species‐specific DNA probe

“…Heiicobacter pylori N C T C 11637T ( T = type strain) was included as a control in all the three sets. The details of the isolates are listed in Table 1 (Malnick et al 1990); W . succinogenes was grown on BHI agar with added supplements (per 30 ml): horse blood 3 ml; and 1.2 ml supplement mixture contain-ing (g 1-' in distilled water): sodium succinate, 2.5; sodium formate, 50; sodium fumarate, 75; sodium pyruvate, 50.…”

Microbial communities profiling in intensive care units expose limitations in current sanitary standards

“…These genera are usually found in moist environments and can show a high risk for HAI in immunocompromised patients. Besides, other genera associated with human microflora were also found in high abundances, such as Lactobacillus (mouth and vaginal flora) and Anaerobiospirillum (human, cat, and dog feces) [27]. NICU mobiles showed a greater abundance of Fusobacterium, Neisseria, Rothia, Granulicatella, and Streptococcus (Fig.…”

Colite ulcéreuse chez un chat : infection à Anaerobiospirillum spp. ?

“…A. thomasii, A. succiniciproducens et autres « anaerobiospirillum-like organisms » sont rapportés comme étant des agents étiologiques de diarrhée, de vomissements et de bactériémie chez l'homme [2]. Ces bactéries ne sont pas isolées dans les selles d'hommes sains [1] [4].…”

“…Le diagnostic de certitude reste une difficulté, d'autant plus qu'il n'existe pas en France, en routine, de moyen diagnostique pour isoler Anaerobiospirillum spp. Les techniques décrites dans la littérature restent réservées à la recherche ou aux milieux hospitaliers : il peut s'agir de kit de réactions enzymatiques [2] ou de techniques d'amplifications moléculaires par polymérisation en chaînes de l'acide ribonucléique ribosomique 16S des bactéries [3][4][5][6]. L'observation microscopique de bactéries spiralées au sein des biopsies manque clairement de spécificité rendant la confirmation du diagnostic délicate.…”