Description of a Rat Palatal Acrylic Plate That Can Be Relined

Meister, Lissandra Matos Bról; Bail, Michele; Pellissari, Cláudia Viviane Guimarães; Ban, Manuella de Cassia Iglesias; Campagnoli, Eduardo Bauml; Jorge, Janaína Habib; Campanha, Nara Hellen

doi:10.1111/jopr.12247

Cited by 5 publications

(8 citation statements)

References 16 publications

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“…To analyze the in vivo disease processes, non-primate and primate animal models are available that constitute a crucial step in the assessment of a dental biomaterial’s behavior. [ 25 ]…”

Section: Discussionmentioning

confidence: 99%

“…For making master impressions of the palate and to affix the palatal appliances, it was obligatory to anesthetize each rat twice. [ 24 25 ] This was attained by the administration of ketamine hydrochloride (80 mg/kg—Ketajet50; Sterkem Pharma, Mumbai, India) and xylazine (10 mg/kg—Xylaxin; Indian Immunological, Telangana, India) intraperitoneally. The working time acquired by this anesthesia was approximately 30 min.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

“…They were now changed to paste diet to prevent the accumulation of food debris under the palatal appliances. [ 24 25 ] The animals used the appliances for 14 days. The weights of the animals were noted just before the appliance insertion (D0) and during euthanasia after 14 days (D14) for each group.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

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Histocompatibility of novel cycloaliphatic comonomer in heat-cured denture base acrylic resin: Histomorphometric analysis in rats

Ajay¹,

Karthigeyan

Arulkumar³

et al. 2020

J Pharm Bioall Sci

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A BSTRACT Background: Prosthodontics is impossible without denture base resins. Allergic reactions to these resins are not uncommon, albeit favorable properties. Monomeric modifications are being done to improve the properties of the material. Tricyclodecane dimethanol diacrylate (TCDDMDA) monomer has been recently identified and experimented as a comonomer with methyl methacrylate (MMA). Aim: This study aimed to investigate the histocompatibility of TCDDMDA comonomer in polymerized resin at 10% and 20% (vol/vol) concentrations in rats by histomorphometric analysis. Materials and Methods: Twenty-four male Wistar rats were randomly divided into the following four groups: NP group (control; n = 6), with no palatal appliance, Groups P0, P10, and P20 were fixed with palatal appliances fabricated of 100% MMA, 10% TCDDMDA + 90% MMA, and 20% TCDDMDA + 80% MMA, respectively. Weights of the animals were recorded just before the appliance placement and after 14 days. The animals were sacrificed, and the palatal tissues were processed for histopathological analysis. Histomorphometric parameters assessed were total epithelial (ET), connective tissue (CT), and keratin layer (KT) thicknesses. Results: No significant difference was observed regarding body weight. Group P0 showed increased ET, CT, and KT when compared to other groups. Bonferroni multiple comparison tests showed a statistically significant difference between all the groups except between P10 and P20 for all the three morphometric parameters. Conclusion: Palatal appliances with TCDDMDA comonomer showed good histocompatibility in rats up to 20% (vol/vol) concentration.

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“…To analyze the in vivo disease processes, non-primate and primate animal models are available that constitute a crucial step in the assessment of a dental biomaterial’s behavior. [ 25 ]…”

Section: Discussionmentioning

confidence: 99%

Section: Aterials and M Ethodsmentioning

confidence: 99%

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Histocompatibility of novel cycloaliphatic comonomer in heat-cured denture base acrylic resin: Histomorphometric analysis in rats

Ajay¹,

Karthigeyan

Arulkumar³

et al. 2020

J Pharm Bioall Sci

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“…* indica diferença significante entre os períodos de 7 e 14 dias para o mesmo grupo (CET) na variável CSB (A2). ;MACDONALD, 1990;1992;JOHNSON et al, 2012;LEE et al, 2011) e retidos por cimentação nos molares (BAIL et al, 2014a;BAIL et al, 2014b;MEISTER et al, 2015). O período experimental de 7 dias de uso dos DIOs foi selecionado por ser um tempo médio no qual é possível se observar em análises histopatológicas a presença de infiltrado inflamatório (MIMA et al, 2010;OLSEN;BONDEVIK, 1978;TRINDADE et al, 2012).…”

Section: Grupounclassified

“…Entretanto, são consideradas restritas clinicamente, pois demonstram efeitos diretos sobre as células e não sobre os complexos tecidos bucais, além de não reproduzirem as condições dinâmicas do ambiente oral (CHAVES et al, 2010;HUANG et al, 2001). Embora investigações in situ tenham sido realizadas para avaliar a biocompatibilidade de resinas acrílicas (KALLUS, 1984;NAGEM-FILHO;CHIODI-NETTO;DE ARAUJO, 1973) MACDONALD, 1990;1992;JOHNSON et al, 2012;LAMB;MARTIN, 1983;LEE et al, 2011;MARTIN, 1989;MEISTER et al, 2015;MORI et al, 1997;OLSEN;BONDEVIK, 1978;MARTIN;SMITH, 1981;1986;TSURUOKA et al, 2008). Por isso, Hotta et al (2016) et al, 1997).…”

Section: Introductionunclassified

Biocompatibilidade in vivo de material resiliente temporário para base de prótese modificado por antimicrobianos para tratamento da estomatite protética

Hotta¹

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Biocompatibility in vivo of temporary soft denture liner for denture base modified by antimicrobials agents for denture stomatitis treatment Temporary resilient denture liners containing antifungal drugs have been suggested as an adjunct treatment for denture stomatitis. However, before clinically using these modified liners in humans, it is important to assess their biocompatibility in animal models. This study evaluated the in vivo biocompatibility of a temporary soft denture liner (Trusoft) modified by antimicrobial agents at their minimum inhibitory concentrations (MICs) for biofilm formation by Candida albicans. Methods: Acrylic intraoral devices (IODs) were individually made for 60 Wistar rats. The rats were divided into the following 6 groups (n=5): 3 control groups (Negative: without IOD; General: IOD without relining; Positive: IOD relined with Trusoft without drugs) and 3 experimental groups (IOD relined with Trusoft modified by drugs at MICs: 0.032 g for nystatin, 0.064 g for chlorhexidine diacetate, and 0.128 g for ketoconazole). The rats with or without the IODs were sacrificed after 7 or 14 days of evaluation. Histopathological qualitative analysis was performed by comparing photomicrographs of histological sections, which were obtained using an optical microscope that transversely covered the inter-molar region. Morphological changes in the epithelium and keratin were quantitatively analyzed by performing computerized planimetry. Quantitative data were analyzed using 2-way ANOVA and Tukey's test (α=0.05). Quantitative analysis showed that only the group with IOD containing ketoconazole significantly decreased the thickness and area of the stratum corneum compared with the other groups (p<0.05), which showed no significant differences between each other (p>0.05). These results were in accordance with those obtained for qualitative analysis. Incorporation of MICs of nystatin and chlorhexidine diacetate in Trusoft did not induce histopathological changes in the palatal mucosa of rats, suggesting the in vivo biocompatibility of this protocol for treating denture stomatitis.

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A novel acrylic resin palatal device contaminated with Candida albicans biofilm for denture stomatitis induction in Wistar rats

et al. 2021

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A novel acrylic resin palatal device contaminated with Candida albicans biofilm for denture stomatitis induction in Wistar rats Denture stomatitis is the most frequent oral lesion in removable prosthesis wearers, with high recurrence rates and a complex treatment. Objective: This study describes a protocol to obtain and to contaminate a palatal device with Candida albicans biofilm that could be used for an animal model of denture stomatitis. Methodology: Acrylic resin devices (N=41) were obtained from impressions of the palates of Wistar rats with individual trays and polyether.The efficacy of microwave irradiation (MW), ultraviolet light (UV), or ultrasonic bath (US) was assessed by colony viability and spectrophotometric analyses (n=5) in order to select the most appropriate method for sterilizing the devices. Then, different devices (n=5) were contaminated with C. albicans and evaluated by CFU/mL determination, scanning electron microscopy, and laser confocal microscopy. Device stabilization was assessed with either autopolymerizing acrylic resins or a self-adhesive resin cement (n=2). The spectrophotometric data were analyzed by one-way ANOVA followed by the Tukey's HSD post-hoc test (α=0.05). Results: MW was the only method capable of sterilizing the devices, and the contamination protocol developed a mature and viable C. albicans biofilm (~1.2 x 10 6 CFU/mL). The self-adhesive resin cement was the best stabilization material. Conclusions: This acrylic resin palatal device was designed to be similar to the clinical situation of contaminated prostheses, with easy manufacturing and handling, effective stabilization, and satisfactory contamination. Thus, the acrylic device can be a valuable tool in the development of denture stomatitis in rats.

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Description of a Rat Palatal Acrylic Plate That Can Be Relined

Cited by 5 publications

References 16 publications

Histocompatibility of novel cycloaliphatic comonomer in heat-cured denture base acrylic resin: Histomorphometric analysis in rats

Histocompatibility of novel cycloaliphatic comonomer in heat-cured denture base acrylic resin: Histomorphometric analysis in rats

Biocompatibilidade in vivo de material resiliente temporário para base de prótese modificado por antimicrobianos para tratamento da estomatite protética

A novel acrylic resin palatal device contaminated with Candida albicans biofilm for denture stomatitis induction in Wistar rats

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