Description of Actinomycetospora chibensis sp. nov., Actinomycetospora chlora sp. nov., Actinomycetospora cinnamomea sp. nov., Actinomycetospora corticicola sp. nov., Actinomycetospora lutea sp. nov., Actinomycetospora straminea sp. nov. and Actinomycetospora succinea sp. nov. and emended description of the genus Actinomycetospora

Abstract

Eight actinomycete strains that form bud-like spore chains were isolated from various samples collected in Japan. Phylogenetically, the isolates formed a single clade with the type strain of Actinomycetospora chiangmaiensis according to 16S rRNA gene sequence analysis. The isolates contained meso-diaminopimelic acid, d-glutamic acid and d- and l- alanine in the cell-wall peptidoglycan, arabinose and galactose as characteristic sugars, phosphatidylcholine and phosphatidylethanolamine as diagnostic phospholipids, MK-8(H4) as the predominant isoprenoid quinone, iso-C16 : 0 as the major cellular fatty acid and DNA G+C contents of 72–74 mol%. Actinomycetospora chiangmaiensis, the type species of the genus Actinomycetospora, was also found to contain MK-8(H4) predominantly in our study, although it was earlier reported to contain MK-9(H4) as the predominant isoprenoid quinone. On the basis of the morphological, physiological, chemotaxonomic, phylogenetic and DNA–DNA hybridization data, we concluded that the isolates can be accommodated in the genus Actinomycetospora with emendation of the description of the genus and are assigned to the following seven novel species: Actinomycetospora chibensis sp. nov. (type strain TT04-21T = NBRC 103694T = KACC 14256T), Actinomycetospora chlora sp. nov. (type strain TT07I-57T = NBRC 105900T = KACC 14252T), Actinomycetospora cinnamomea sp. nov. (type strain IY07-53T = NBRC 105527T = KACC 14250T), Actinomycetospora corticicola sp. nov. (type strain 014-5T = NBRC 103689T = KACC 14253T), Actinomycetospora lutea sp. nov. (type strain TT00-04T = NBRC 103690T = KACC 14254T), Actinomycetospora straminea sp. nov. (type strain IY07-55T = NBRC 105528T = KACC 14251T) and Actinomycetospora succinea sp. nov. (type strain TT00-49T = NBRC 103691T = KACC 14255T).

Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample

“…Interestingly, the previously reported Actinomycetospora species were mainly isolated from subtropical/tropical region. 9,10 A similar tendency was also reported by González et al 3 It is considered that the abundance or diversity of Actinomycetospora correlated to the climate. However, ecological futures of lichenassociated actinomycetes, including Actinomycetospora were not clarified yet.…”

“…The 16S rRNA gene sequence similarities to members of the genus Actinomycetospora ranged from 97.0 to 98.4%. [8][9][10] The phylogenetic tree constructed with 16S rRNA gene sequence data of members of the genus Actinomycetospora and related genera using the neighbor-joining method showed that strain IR73-Li102 T formed a monophyletic clade with A. chiangmaiensis, A. corticola and A. rishiriensis (Figure 1). Moreover, this clade was strongly supported by the maximum-likelihood and maximum parsimony trees with high bootstrap values.…”

“…Previously, several rare actinomycetes were isolated from lichen samples collected in Japan, and were proposed as new taxa, named Nocardioides exalbidus, Leifsonia lichenia, and Actinomycetospora rishiriensis. [6][7][8] The genus Actinomycetospora was originally proposed by Jiang et al 9 as a member of the family Pseudonocardiaceae, and was later amended by Tamura et al 10 At the time of writing, nine Actinomycetospora species have been characterized by polyphasic taxonomic approaches. The type strain of the genus Actinomycetospora, A. chiangmaiensis, was originally isolated from a soil sample in northern Thailand, whereas the other species were from soil, tree bark, and lichen samples collected in Japan.…”

“…The type strain of the genus Actinomycetospora, A. chiangmaiensis, was originally isolated from a soil sample in northern Thailand, whereas the other species were from soil, tree bark, and lichen samples collected in Japan. [8][9][10] During a screening for lichen-associated actinomycetes, we isolated strain IR73-Li102 T from a crustose lichen sample collected from Iriomote Island, Okinawa, Japan. The aim of the present study was to determine the taxonomic position of strain IR73-Li102 T using a polyphasic taxonomic approach, which included chemotaxonomic, morphological, physiological, molecular and genomic characterizations.…”

Actinomycetospora rhizophila sp. nov., an actinomycete isolated from rhizosphere soil of a peace lily (Spathi phyllum Kochii)

“…The genus Actinomycetospora was first established by Jiang et al (2008) and its description was later emended by Tamura et al (2011b) and Zhang et al (2014). At the time of writing, the genus encompasses 11 species with validly published names (http://www.bacterio.net/actinomycetospora.html), including the recently described Actinomycetospora atypica (Zhang et al , 2014).…”