Description of the Sperm and Spermatheca of Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) for the Differentiation of Mated and Unmated Females

Román-Ruiz, A.K.; Michel, Bruno; Dufour, Bernard Pierre; Rojas, Julio C.; Cruz‐López, Leopoldo; Barrera, Juan F.

doi:10.1093/aesa/sax033

Cited by 5 publications

(10 citation statements)

References 22 publications

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“…Close to the end of the common oviduct, on the right side, in a dorsal position was an ampulla-shaped accessory gland; between the accessory glands (very close to each other) was a hook-shaped spermatheca. The micro-CT rendered images obtained here were totally coincident with light microscopic images obtained following dissection 10,11,14 . Details of the hooked distal part of the spermatheca, spermatic gland, and the spermathecal muscles described recently 14 were enhanced using micro-CT (Fig.…”

Section: Discussionsupporting

confidence: 68%

“…The micro-CT rendered images obtained here were totally coincident with light microscopic images obtained following dissection 10,11,14 . Details of the hooked distal part of the spermatheca, spermatic gland, and the spermathecal muscles described recently 14 were enhanced using micro-CT (Fig. 10D; Supplementary Video S8).…”

Section: Discussionsupporting

confidence: 68%

“…Román-Ruiz et al . 14 first photographed and described the spermatic gland as a “cell agglomeration on the curved distal end of the spermatheca”, but they added that “a further study will be necessary to confirm this finding”. The micro-CT reconstructions allowed us to visualize the structure previously identified as the spermatic gland (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The insect has also been reported to have infested vast areas, e.g., over 715,000 ha in Colombia by 1998 7 and more than 800,000 ha by 2002 8 . Even though > 1,800 papers have been published on the coffee berry borer 9 , knowledge about its internal anatomy is restricted to a handful of papers that use dissection techniques 10–14 .…”

Section: Introductionmentioning

confidence: 99%

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Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

Alba-Alejandre

Alba‐Tercedor

Vega

2019

Sci Rep

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traditionally, the study of anatomy in insects has been based on dissection techniques. Microcomputed tomography (micro-ct) is an X-ray based technique that allows visualization of the internal anatomy of insects in situ and does not require dissections. We report on the use of micro-ct scans to study, in detail, the internal structures and organs of the coffee berry borer (Hypothenemus hampei), the most damaging insect pest of coffee worldwide. Detailed images and videos allowed us to make the first description of the aedeagus and the first report of differences between the sexes based on internal anatomy (flight musculature, midgut shape, hindgut convolutions, brain shape and size) and external morphology (lateral outline of the pronotum and number of abdominal tergites). This study is the first complete micro-ct reconstruction of the anatomy of an insect and is also the smallest insect to have been evaluated in this way. High quality rendered images, and additional supplementary videos and 3D models are suitable for use with mobile devices and are useful tools for future research and as teaching aids. Ever since the introduction of coffee from Africa to various continents, it has become an important commodity not only to producing countries, but also to billions of consumers that take delight on its flavour, aroma, and stimulatory effects 1. Amongst the many agronomic problems faced by coffee producers, insect pests and plant pathogens are of paramount importance 2. Climate change is also posing an enormous challenge to the continuity of commercial coffee production and to the survival of wild coffee species 3,4. The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae: Cryphalini), is the most economically important insect pest of coffee due to its cryptic life cycle inside the coffee berry, which makes it quite hard to manage 5. The insect is currently present in most coffee producing countries 5 and worldwide losses are likely over US$500 million, with yearly losses in Brazil alone estimated at US$215-358 million 6. The insect has also been reported to have infested vast areas, e.g., over 715,000 ha in Colombia by 1998 7 and more than 800,000 ha by 2002 8. Even though > 1,800 papers have been published on the coffee berry borer 9 , knowledge about its internal anatomy is restricted to a handful of papers that use dissection techniques 10-14. Insect dissection techniques were first used more than 400 years ago by Aldrovandi 15 and Malpighi 16. Even though dissections have most definitely been useful, resulting in thousands of papers on the internal anatomy of insects, they result in an inevitable distortion of the internal structures and organs. A relatively new technique based on X-rays, known as micro-computed tomography (micro-CT), allows visualization of the insect's internal anatomy in situ, without the need for dissection; results have been validated by comparing them with classical destructive methodologies 17,18. We present a detailed study of all the anatomical st...

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Section: Discussionsupporting

confidence: 68%

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

Alba-Alejandre

Alba‐Tercedor

Vega

2019

Sci Rep

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show abstract

“…Clarification technique was only used for virgin females to facilitate the fixation of the dye. It consisted of immerse spermatheca in 10% KOH for 15 min, then in distilled water for 15–20 min, and lastly in 95% ethanol for 15 min (Roman-Ruiz et al ., 2017).…”

Section: Methodsmentioning

confidence: 99%

The singular insemination status of Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) females during the inter-harvest season of a coffee crop

Román-Ruiz

Barrera

Cruz‐López

et al. 2018

Bull. Entomol. Res.

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The coffee berry borer, Hypothenemus hampei (Ferrari), can survive in residual coffee berries during the inter-harvest period, while new fructification only appears 2–3 months after the last harvest. The dispersal of colonizing females is an adaptation that enables the life cycle of the species to go ahead whenever his flight aptitude allows. This paper focuses on accurately determining the rate of inseminated females ready to reproduce when emerging from residuals berries to colonize new ones, which constitutes a characteristic of the live cycle far from common in Curculionidae. We dissected females caught in traps baited with a mixture of alcohols during the inter-harvest season, females from infested residual berries collected from branches, and virgin females obtained from pupae reared individually in the laboratory. After microscopic preparation with Giemsa stain, spermathecae were observed to identify the physiological status of each specimen. Out of the females found in the traps, 98.4% displayed recent and abundant insemination and 1.6% sporadic insemination. In contrast, in residual berries, most of females were recently inseminated (84.5%), followed by virgin females (10.5%) and older inseminated females (5%). In addition, the flight tests of the virgin females were negative. These results indicate that all colonizing females were inseminated, ready for flying and oviposition, females inside residual berries showed different physiological status, and virgin females could not migrate since they could not flight. The large number of inseminated females inside the residual berries, and the capacity of migrating females to colonize and reproduce, suggest that it is necessary to control residual berries and use traps to stop the dispersal and reproduction of this pest.

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Morphology of testis, sperm, and spermatheca in two capable hybridized termite species indicates no interspecific reproductive isolation

Khan

Suleman

et al. 2022

Int J Trop Insect Sci

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Description of the Sperm and Spermatheca of Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) for the Differentiation of Mated and Unmated Females

Cited by 5 publications

References 22 publications

Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

The singular insemination status of Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) females during the inter-harvest season of a coffee crop

Morphology of testis, sperm, and spermatheca in two capable hybridized termite species indicates no interspecific reproductive isolation

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