Description of two Serratia marcescens associated mastitis outbreaks in Finnish dairy farms and a review of literature

Friman, Mari; Eklund, Marjut; Pitkälä, Anna; Rajala‐Schultz, Päivi J.; Rantala, Merja

doi:10.1186/s13028-019-0488-7

Cited by 25 publications

(27 citation statements)

References 35 publications

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“…In the Irish herds, all outbreaks had been caused by the same P. aeruginosa strain. There are some similarities with mastitis outbreaks caused by Serratia (S.) marcescens in Finnish dairy farms [ 34 ]. Epidemiological characterization of isolates from two outbreaks revealed that IMIs were associated with contaminated teat dips containing a tertiary alkyl amine, n,n-bis (3-aminopropyl) dodecyl amine.…”

Section: Discussionmentioning

confidence: 99%

Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd

Schauer

Wald

Urbantke

et al. 2021

Animals

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The present study describes an outbreak of Pseudomonas (P.) aeruginosa mastitis in a 20-cow dairy herd where throughout genotyping of isolates reusable udder towels were identified as the source of infection. Sampling of cows during three herd surveys and bacteriological culturing showed that P. aeruginosa was isolated from nine cows with a total of 13 infected quarters. Mastitis occurred as mild clinical or subclinical infection. P. aeruginosa was additionally isolated from a teat disinfectant solution, containing N-(3-aminopropyl)-N-dodécylpropane-1,3-diamine 1 as active component, and microfiber towels used for pre-milking teat preparation. Disc diffusion antimicrobial resistance testing revealed that all isolates were susceptible to piperacillin, piperacillin-tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem, tobramycin, amikacin, and ciprofloxacin. Thirty-two isolates of milk samples and 22 randomly selected isolates of one udder towel and of the teat disinfectant solution were confirmed as P. aeruginosa with matrix-assisted laser desorption, ionization time-of-flight mass spectrometry (MALDI Tof MS). Isolates were further characterized with rep-PCR and randomly amplified polymorphic DNA (RAPD) as well as with multiple locus variable-number tandem repeat analysis (MLVA). Results obtained in this study suggested that one single strain was responsible for the whole outbreak. The transmission occurred throughout a contaminated teat cleaning solution as a source of infection. The farmer was advised to change udder-preparing routine and to cull infected cows.

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Section: Discussionmentioning

confidence: 99%

Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd

Schauer

Wald

Urbantke

et al. 2021

Animals

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“…may cause mild to severe illnesses [ 5 ]. Microorganisms may contaminate raw milk during milking, transportation, storage and processing, which pose a serious threat to the quality and safety of milk and milk products [ 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Impacts of Milking and Housing Environment on Milk Microbiota

Meng

Liu

et al. 2020

Animals

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The aim of the present study was to determine the effects of farming environments on microbiota in raw milk and to assess the relationship among microbes by 16S rRNA sequencing methods. Samples of raw milk, cow trough water, teat dip cup, teat, teat liner, dairy hall air, cowshed air, feces, feed, and bedding from two farms were collected. The two highest abundant bacterial groups of Moraxellaceae and Staphylococcaceae were found in milk and teat liner samples, respectively, at Zhengzhou farm, Henan Province. Moreover, the two highest abundant bacterial groups of Enterobacteriaceae and Moraxellaceae were found in milk and teat dip cup samples, respectively, at Qiqihar farm, Heilongjiang Province. Source Tracker analysis revealed that the teat liner and teat dip cup were the most important contributors of microbes in milk samples at Zhengzhou farm and Qiqihar farm, respectively, which could be attributed to the management level of the farm. Therefore, disinfection and cleaning procedures should be developed to improve the quality of raw milk.

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“…marcescens is known to be associated with abortion [21] and mastitis outbreaks in dairy cows [22], inflammation of the digestive tract in hummingbirds [23], and necrotizing fasciitis in dogs [24]. Both P. mirabilis and S. marcescens are opportunistic pathogens in humans [25] and can be potentially transmitted through contaminated food [26].…”

Section: Advances In Biological Sciences Research Volume 14mentioning

confidence: 99%

Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)

Masrukhin¹,

Saputra²,

Rizal³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Antibiotic resistance is one of the most serious health issues in the world which is commonly attributed to the overuse of antibiotics whether in human or animal treatment. The dispersion of antibiotic resistance genes not only found in bacteria from the clinical sample but also found in wildlife animals. Therefore, the aim of this research is to isolate, identify and perform antibiotic resistance assay in bacterial isolates from fruit bat Chironax melanocephalus. Animal samples were taken by using a mist net installed in one of the spots in Gunung Halimun Salak National Park (TNGHS). Bacteria sample was taken using an oral and rectal swab. The bacteria were then isolated in the laboratory by using xylose lactose deoxycholate (XLD) agar medium. There were two bacteria successfully isolated and identified using 16s rDNA amplification. The identification was performed by comparing the 16s rDNA genes to the two online databases i.e BLAST-N in Genebank and 16s-Based ID in the EzBioCloud database. According to the identification results, isolate 4A.1 was identified as Proteus mirabilis while isolate 5.B.2 is identified as Serratia marcescens. Then antibiotic susceptibility test was performed using the disk diffusion method using four different antibiotics disk i.e oxacillin, cefoxitin, amikacin and amoxicillinclavulanic acid. The resistance profile of each bacteria is different between P. mirabilis and S. marcescens in each antibiotic. However, all isolates were found susceptible against amikacin 30 µg.

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Description of two Serratia marcescens associated mastitis outbreaks in Finnish dairy farms and a review of literature

Cited by 25 publications

References 35 publications

Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd

Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd

Impacts of Milking and Housing Environment on Milk Microbiota

Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)

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