Desensitized morphological and cytokine response after stretch-shortening muscle contractions as a feature of aging in rats

Rader, Erik P.; Layner, Kayla N.; Triscuit, Alyssa M.; Kashon, Michael L.; Gu, Ja K.; Ensey, James; Baker, Brent A.

doi:10.1016/j.exger.2015.09.020

Cited by 9 publications

(27 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Changes in muscle morphology assessed in hematoxylin and eosin stained slides, along with changes in performance, are published in a companion paper (Rader and others 2015). …”

Section: Resultsmentioning

confidence: 99%

“…The studies describing the effects of iSSCs were performed in Sprague Dawley rats. A companion study (Rader and others 2015) analyzed the effects of iSSCs in Fisher 344 × Brown Norway F1 rats, a model commonly used to study the effects of aging, to determine if exposure also resulted in anatomical, physiological and molecular changes associated with muscle injury and dysfunction. This study found that 3 days after exposure to iSSCs, there was a reduction in force production in both young and old rats.…”

Section: Introductionmentioning

confidence: 99%

“…By 10 days after the exposure, muscle forces had returned to baseline levels in young rats, but there was still a force deficit in old rats. In addition, changes in gene transcription were different between young and old rats (Rader and others 2015). These age-related changes may be due to a delayed response to healing in older animals, or they could be the result of changes due to pain or discomfort that may occur as a result of the exposure.…”

Section: Introductionmentioning

confidence: 99%

“…Hyperalgesia due to muscle inflammation via adjuvant injection or eccentric contractions has been associated with changes in CGRP concentrations in injured muscle and DRG (Bulling and others 2001; Dessem and others 2010). Because aged animals often show a delayed or attenuated response to muscle injury (Cutlip and others 2009; Hollander and others 2010; Rader and others 2015), there may be changes in the development of hyperalgesia in response to iSSCs, and changes in CGRP may accompany or be a marker of this hyperalgesia. Therefore, in this study, we tested the hypothesis that iSSC-induced muscle injury would result in hyperalgesia in the exposed limb, and that aging may affect the development of hyperalgesia.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Changes in the expression of calcitonin gene-related peptide after exposure to injurious stretch-shortening contractions

Johnson

Miller

Baker

et al. 2016

Experimental Gerontology

View full text Add to dashboard Cite

One of the factors that can result in musculoskeletal injuries, and time off work, is exposure to repetitive motion. The goal of this study was to determine if skeletal muscle injury induced by exposure to injurious stretch-shortening cycles (iSSCs), resulted in hyperalgesia in the hind limb and changes in calcitonin-gene related peptide (CGRP) immunolabeling in the dorsal root ganglia (DRG) in young and old male rats. Methods Young (3 mo) and old (30 mo) male Fisher 344 × BN F1 rats were anesthetized with isoflurane and the left hind limbs were exposed to 15 sets of 10 SSCs. Control animals were exposed to a single bout of SSCs of equal intensity. Sensitivity to mechanical stimulation was assessed using von Frey filaments prior to beginning the experiment, and on days 2 and 9 following exposure to iSSCs. Rats were euthanized one, 3 or 10 days after the exposure. The ipsilateral DRG were dissected from the L4-5 region of the spine, along with the left tibialis anterior (LTA) muscle. Results Rats exposed to iSSCs were more sensitive to mechanical stimulation than control rats 2 days after the exposure, and showed a reduction in peak force 3 days after exposure. Changes in sensitivity to pressure were not associated with increases in CGRP labeling in the DRG at 3 days. However, 9 days after exposure to iSSCs, old rats still displayed an increased sensitivity to mechanical stimulation, and this hyperalgesia was associated with an increase in CGRP immunolabeling in the DRG. Young rats exposed to iSSC did not display a change in CGRP immunolabeling and sensitivity to mechanical stimulation returned to control levels at 10 days. Conclusions These findings suggest that hyperalgesia seen shortly after exposure to iSSC is not influenced by CGRP levels. However, in cases where recovery from injury may be slower, as it is in older rats, CGRP may contribute to the maintenance of hyperalgesia.

show abstract

“…Changes in muscle morphology assessed in hematoxylin and eosin stained slides, along with changes in performance, are published in a companion paper (Rader and others 2015). …”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Changes in the expression of calcitonin gene-related peptide after exposure to injurious stretch-shortening contractions

Johnson

Miller

Baker

et al. 2016

Experimental Gerontology

View full text Add to dashboard Cite

show abstract

“…These examples are initiated by focal mechanical/physical insult that induces overt injury [23][24][25] to the underlying micro-level structures such as sarcomeres [26], the excitation-contraction coupling system [27] and the surrounding interstitium [28]. Additionally, following the initial induction of injury, there is a putative secondary injury phase comprising the biological response that includes significant gradations of edema (i.e., swelling), inflammation, degeneration, regeneration, and repair and remodeling [29][30][31][32][33][34]. However, this phenomenon is very distinct from cases where maladaptation results, such is the case in physical work and exercise-induced events, and is accompanied by transient soreness and discomfort following loading of soft tissue [12,35,36].…”

Section: Musculoskeletal Disorders (Msds): Dynapenia Sarcopenia Andmentioning

confidence: 99%

Efficacy of Age-Specific High-Intensity Stretch-Shortening Contractions in Reversing Dynapenia, Sarcopenia, and Loss of Skeletal Muscle Quality

Baker

2018

JFMK

View full text Add to dashboard Cite

During the aging process, skeletal muscle performance and physiology undergoes alterations leading to decrements in functional capacity, health-span, and independence. Background: The utility and implementation of age-specific exercise is a paramount research agenda focusing on ameliorating the loss of both skeletal muscle performance and physiology; yet, to date, no consensus exists as to the most appropriate mechanical loading protocol design or overall exercise prescription that best meets this need. Thus, the purpose of this review is to highlight the most optimal type of exercise presently available and provide the most current, evidence-based findings for its efficacy. The hypothesis that high-intensity, stretch-shortening contractions (SSCs)-a form of "resistance-type exercise" training-present as the preferred exercise mode for serving as an intervention-based modality to attenuate dynapenia, sarcopenia, and decreased muscle quality with aging, even restoring the overall youthful phenotype, will be demonstrated. Conclusions: Appreciating the fundamental evidence supporting the use of high-intensity SSCs in positively impacting aging skeletal muscle's responsivity and their use as a specific and sensitive countermeasure is crucial. Moreover, from an applied perspective, SSCs may improve skeletal muscle quality and rejuvenate health-span and, ultimately, lead to augmented functional capacity, independence, and quality of life concomitant with decreased morbidity.

show abstract

An Old Problem: Aging and Skeletal-Muscle-Strain Injury

Baker¹

2017

Journal of Sport Rehabilitation

Self Cite

View full text Add to dashboard Cite

Understanding the fundamental evidence regarding how aging influences the responsivity of skeletal muscle to strain injury is vital for informing how clinicians approach and implement preventive strategies, as well as therapeutic interventions. From a practical perspective, maintaining or improving the overall health and tissue quality of skeletal muscle as one ages will positively affect skeletal muscle's safety threshold and responsivity, which may reduce incidence of injury, improve recovery time, and lessen overall fiscal burdens.

show abstract

Desensitized morphological and cytokine response after stretch-shortening muscle contractions as a feature of aging in rats

Cited by 9 publications

References 46 publications

Changes in the expression of calcitonin gene-related peptide after exposure to injurious stretch-shortening contractions

Changes in the expression of calcitonin gene-related peptide after exposure to injurious stretch-shortening contractions

Efficacy of Age-Specific High-Intensity Stretch-Shortening Contractions in Reversing Dynapenia, Sarcopenia, and Loss of Skeletal Muscle Quality

An Old Problem: Aging and Skeletal-Muscle-Strain Injury

Contact Info

Product

Resources

About