Desenvolvimento de biomaterial composto por hidroxiapatita e clorexidina para aplicação na cavidade oral

Abstract

ResumoA hidroxiapatita (HAp) está presente no organismo humano nos tecidos ósseos e dentários, em que do total da massa de cada tecido 30 a 70% correspondem a HAp, podendo ser aplicada como revestimentos de próteses ou até substituição de pequenas partes ósseas. Assim, o objetivo foi sintetizar HAp por precipitação, incorporar clorexidina (CHX) e avaliar as propriedades microbianas. A caracterização foi realizada por DRX, FTIR, TG/DTG, MET e MEV. A CHX foi incorporada à HAp por adsorção, variando a quantidade incorporada. O difratograma apresentou picos em 25,9º, 31,5°, 32,2º e 32,7º (2θ) referentes aos planos (002), (211), (112) e (300) da HAp. O espectro de FTIR mostrou banda em 1050 cm -1 correspondente à deformação dos grupos fosfatos e deformações de grupos CH 2 em 1485 e 1445 cm -1 da clorexidina, que apresentou morfologia porosa e cilíndrica. O biomaterial apresentou excelentes propriedades antimicrobianas frente a Streptococcus mutans, Staphylococcus aureus e Escherichia coli. Palavras-chave: hidroxiapatita, clorexidina, atividade antimicrobiana. AbstractHydroxyapatite (HAp) is present in the human body in bone and dental tissues, which of the total mass of each tissue 30 to 70% correspond to HAp, and can be applied as coatings of prostheses or replacement of small bone parts. Thus, the objective was to synthesize HAp by precipitation, to incorporate chlorhexidine (CHX) and to evaluate the microbial properties. The characterization was performed by XRD, FTIR, TG/DTG, TEM and SEM. CHX was incorporated into the HAp by adsorption, varying the amount incorporated. The XRD pattern showed peaks at 25.9º, 31.5º, 32.2ºand 32.7º (2θ) for planes (002), (211), (112) and (300) of HAp. The FTIR spectrum showed band at 1050 cm -1 corresponding to the deformation of the phosphate groups and deformations of CH 2 groups at 1485 and 1445 cm -1 of chlorhexidine, which presented porous and cylindrical morphology. The biomaterial showed excellent antimicrobial properties against Streptococcus mutans, Staphylococcus aureus and Escherichia coli.

Chitosan-Based Biomaterial, Calcium Hydroxide and Chlorhexidine for Potential Use as Intracanal Medication

“…The results were measured using the mean of the three inhibition zones, which were measured 24 h later without subtracting the diameter of the discs. The samples whose inhibition zones grew less than 10 mm were classified as inactive, while those whose halos varied from 10 to 15 mm were classified as active, and those with results above 15 mm were classified as highly active [29][30][31].…”

“…Its bactericidal effect results from the neutralization of the substrates of microorganisms present inside the root canals, with the continuous release of hydroxyl ions. Chlorhexidine at high concentrations is a bactericidal antimicrobial, but at lower concentrations, it becomes bacteriostatic [31,50]. It has been demonstrated that the application of chlorhexidine in root canals for 7 days brings significant results regarding the reduction of bacteria [51].…”

Synthesis and Characterization of Cassava Gum Hydrogel Associated with Chlorhexidine and Evaluation of Release and Antimicrobial Activity

“…Another band characteristic of the deformation of CH 2 groups was observed in the region of 1485 cm −1 . [ 47 ]…”

O Uso De Fluido Corporeo Simulado Em Ensaios in Vitro Para Avaliar O Potencial Osteocondutivo Em Ligas De Magnésio: Uma Breve Revisão.

“…A hidroxiapatita mineral existe naturalmente na forma de apatita de cálcio [Ca10(PO4)6(OH)2] e possui semelhança química com os componentes minerais dos ossos e dentes. Seu uso é largamente explorado em biomateriais como aplicações médicas, tais como revestimento de próteses e substituições de pequenas partes ósseas (DOS SANTOS et al, 2019).…”