Desferrioxamine-chelatable iron, a component of serum non–transferrin-bound iron, used for assessing chelation therapy

Non Transferrin Bound Iron: Nature, Manifestations and Analytical Approaches for Estimation

“…Clinically it has been proven that the accessibility of different iron chelators vary in assorted clinical conditions which indirectly support the heterogeneity of NTBI. For example homochromatic sera with 80-85 % Tf saturation showed no detectable DFO-chelatable iron where as Breuer et al [11,46] found DCI in the similar patient group when mobilizer like oxalate was applied. In case of thalassemic sera with [90 % Tf saturation, both the forms of DCI i.e.…”

“…The term ''BDI'' have been applied to address the forms of NTBI which are detected when associated with bleomycin [10]. ''Desferrioxamine-chelatable iron'' (DCI) was the term used by Breuer for the forms of NTBI which are chelatable with the well known iron chelator desferrioxamine [11]. However the abbreviation ''DCI'' had been used by the same researcher and his colleagues later as ''directly chelatable iron'' to indicate the NTBI forms which are readily chelated with desferrioxamine without the assistance of mobilizer [12].…”

“…Oxidative stress has also been documented as a cause as well as consequence of free iron, where the stable iron compound like iron sulphur proteins, ferritin and even haem release NTBI [34][35][36][37][38][39][40][41][42]. As shown in Table 1 free iron has been well documented as NTBI or other relevant subfractions in various iron overload conditions like hemochromatosis and thalassemia [11,20,[43][44][45].…”

“…The assay also included 10 mM oxalate as mobilizer and 0.1 mM gallium(III) as blocking agent. After the use of Fl-aTf, the use of fluorescent labeled desferrioxamine (Fl-DFO) was successfully experimented by the same researcher to determine the ''DFO chelatable iron'' or DCI without the assistance of mobilizer [11]. In this method fluorescent quenching of Fl-DFO is measured after its binding with iron.…”

Iron overload thalassemic cardiomyopathy: Iron status assessment and mechanisms of mechanical and electrical disturbance due to iron toxicity

“…Currently, however, it is commonly used only in research studies. Various methods and protocols used in the determination of NTBI have been proposed, but no consensus has been established (28)(29)(30)(31)(32).…”