Desiccation survival of entomopathogenic nematodes of the genusHeterorhabditis

Liu, Q. Z.; Glazer, I.

doi:10.1007/bf02981828

Cited by 29 publications

(17 citation statements)

References 18 publications

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“…Both steinernematid species have been reported to be more desiccant tolerant than several other species (Glazer, 2002). The study of Shapiro-Ilan et al (2005) reported no differences in survival of H. bacteriophora, S. carpocapsae and S. feltiae when they were exposed to 85% RH for 48 h. Liu and Glazer (2000) showed that H. bacteriophora requires a more gradual and longer period of adaptation than that of steinernematids. Preconditioning infective juveniles, for example to 97% RH, could improve survival levels (Womersley, 1990;Menti et al, 1997).…”

Section: Discussionmentioning

confidence: 93%

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Ecological characterization of entomopathogenic nematodes isolated in stone fruit orchard soils of Mediterranean areas

Morton

Pino

2009

Journal of Invertebrate Pathology

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Section: Discussionmentioning

confidence: 93%

“…Tolerance to desiccation was evaluated based on procedures described by Liu and Glazer (2000). Approximately 10,000 IJs were concentrated by vacuum filtration onto 50 mm diameter filter paper disks (Whatmann No.…”

Section: Desiccationmentioning

confidence: 99%

Ecological characterization of entomopathogenic nematodes isolated in stone fruit orchard soils of Mediterranean areas

Morton

Pino

2009

Journal of Invertebrate Pathology

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“…Surrey and Wharton (1995) tested the desiccation survival of H. zealandica and found poor viability once water had been lost from the substrate. Liu and Glazer (2000) determined the desiccation tolerance of Israeli Heterorhabditid populations. Substantial differences in survival ability were observed among IJs of different Heterorhabditid populations.…”

Section: Discussionmentioning

confidence: 99%

Characterization of populations of entomopathogenic nematodes isolated at diverse sites across Israel

Salame

Glazer

Miqaia³

et al. 2009

Phytoparasitica

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Entomopathogenic nematodes (EPNs) are known as effective biological agents against soildwelling stages of insect pests. In the present study new EPN populations from the Steinernematidae and Heterorhabditidae families were isolated at different locations in Israel. The newly isolated populations were subjected to a series of bioassays for beneficial traits, for selection of a superior isolate for biological control of insect pests. Nematode motility was measured in sand columns, with and without the susceptible insect Galleria mellonella. Infectivity was evaluated using an invasion rate assay, as well as a dose response assay in which LD 50 values were calculated. Nematode tolerance to environmental stresses was examined in heat and desiccation tolerance assays. The reproductive potentials of the new isolates were assessed in cadavers of G. mellonella. All Steinernematid populations were identified as Steinernema feltiae and the Heterorhabditids as Heterorhabditis sp. Large variation between the various populations was recorded in all assays. In order to identify a superior isolate, each strain was scored as 1 if its performance did not differ significantly from the highest level recorded in the particular assay; −1 if its performance did not differ significantly from the lowest level; and 0 if its performance was between the highest and lowest levels or did not differ from either. The scores for the different traits were then summed for each population and the totals were compared. The best score (+4) was obtained for a S. feltiae population isolated in northern Israel. These results will be used for the utilization of the new population as a biological control agent.

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“…However, 84% of IP variation was 201 explained by SR. The results means that the loss of MCp did not have a direct effect on SR and IP 202 on G. mellonella, however IJ's survival rate directly influences the infectivity on G. mellonella 203 larvae.Figure 3shows the MCp behavior versus SR. Data were fitted to a SR versus IP on G. mellonella; data were fitted to an exponential equation of the between moisture and IJ survival rate and survival rate with infectivity.Freckman (1981),Silver et al (1995) andLiu and Glazer (2000) reported that 211 induction of entomopathogenic nematodes to anhydrobiosis improved survival. The ability level 212 of the EPN to reduce their metabolism under desiccation in DE pellets is another biological factor 213 that may be involved in their survival rate.…”

mentioning

confidence: 96%

“…1472.6. Measurement of moisture content in infective juvenilesThe drying method was used to determine the moisture content of the IJ (MCIJ); 1000±50 IJ were 149 placed on nitrocellulose paper disks (47 mm in diameter and 0.45 µm pore size) to remove the 150 excess surface water by vacuum suction (Millipore, Bedford, MA)(Liu & Glazer, 2000;Charwat, Fisher, & Wyss , 2002). Then the IJs were subjected to a drying process at 105 °C for 24 h in a…”

mentioning

confidence: 99%

Effect of moisture evaporation from diatomaceous earth pellets on storage stability ofSteinernema glaseri

Cortés-Martínez

Ruiz-Vega

Matadamas-Ortiz

et al. 2016

Biocontrol Science and Technology

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A c c e p t e d M a n u s c r i p t 2 Induction of anhydrobiosis and storage stability of entomopathogenic nematodes are influenced by moisture availability. Decreasing moisture content in diatomaceous earth pellets containing Steinernema glaseri NJ-43 strain and its effect on survival time and infectivity of the nematode was determined. Pelletisation was performed in a vortex mixer, using diatomaceous earth Celite ® 209 as desiccant material. Pellets were stored at room temperature (23±2 °C) and high relative humidity (96-100%). Nematode survival and infectivity against last instar greater wax moth, Galleria mellonella, were tested daily. Initial average and average equilibrium moisture content in pellets were 66.7% and 13.6%, respectively, and the IJ's mean survival time was 8.8 days. A moisture transfer model based on diffusion and evaporation was evaluated to predict moisture fluctuations within the pellets. We concluded that 84% of variation in S. glaseri infectivity on G. mellonella larvae was explained by the survival of the nematode whereas 52% of variation in S. glaseri survival was explained by the loss of moisture from the pellets. The moisture transfer model achieved 78% reliability in predicting moisture content and fluctuations. Therefore, the mechanisms of moisture diffusion and evaporation from the surface to the surrounding atmosphere contribute significantly to moisture loss from the pellets.

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Desiccation survival of entomopathogenic nematodes of the genusHeterorhabditis

Cited by 29 publications

References 18 publications

Ecological characterization of entomopathogenic nematodes isolated in stone fruit orchard soils of Mediterranean areas

Ecological characterization of entomopathogenic nematodes isolated in stone fruit orchard soils of Mediterranean areas

Characterization of populations of entomopathogenic nematodes isolated at diverse sites across Israel

Effect of moisture evaporation from diatomaceous earth pellets on storage stability ofSteinernema glaseri

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