Design an immobilized lipase enzyme for biodiesel production

Jegannathan, Kenthorai Raman; Jun-Yee, Leong; Eng-Seng, Chan; Ravindra, Pogaku

doi:10.1063/1.3256191

Cited by 15 publications

(6 citation statements)

References 29 publications

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“…Moreover, the enzyme, except for its catalytic action, also contributes to the separation and recovery of the reaction products [352,355]. Thereafter, the use of enzyme catalysts will consist of a sustainable and eco-friendly transesterification method [359]. Among the existing catalysts, the most recent development with great prospect is the use of nanomaterials as catalysts in the transesterification process.…”

Section: Transesterificationmentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

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Section: Transesterificationmentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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“…Contrary to chemical catalysts, enzymes do not form soaps and catalyze esterification of FFA and TG in one step without any need of the washing step. On the other hand, the major disadvantages of the enzymatic transesterification are its slower reaction rate and possible enzyme inactivation by methanol [ 27 , 62 , 82 ]. Lipase is an enzyme capable of catalyzing methanolysis reactions.…”

Section: Biodieselmentioning

confidence: 99%

“…Cao [ 84 ] mentioned that an immobilized enzyme has to perform two essential functions, namely, the noncatalytic functions that are designed to aid separation and the catalytic functions that are designed to convert the targeting substrates within a desired time. This is in addition to the fact that the process is environmentally friendly and more sustainable [ 82 ].…”

Section: Biodieselmentioning

confidence: 99%

A Review of Enzymatic Transesterification of Microalgal Oil-Based Biodiesel Using Supercritical Technology

et al. 2011

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Biodiesel is considered a promising replacement to petroleum-derived diesel. Using oils extracted from agricultural crops competes with their use as food and cannot realistically satisfy the global demand of diesel-fuel requirements. On the other hand, microalgae, which have a much higher oil yield per hectare, compared to oil crops, appear to be a source that has the potential to completely replace fossil diesel. Microalgae oil extraction is a major step in the overall biodiesel production process. Recently, supercritical carbon dioxide (SC-CO2) has been proposed to replace conventional solvent extraction techniques because it is nontoxic, nonhazardous, chemically stable, and inexpensive. It uses environmentally acceptable solvent, which can easily be separated from the products. In addition, the use of SC-CO2 as a reaction media has also been proposed to eliminate the inhibition limitations that encounter biodiesel production reaction using immobilized enzyme as a catalyst. Furthermore, using SC-CO2 allows easy separation of the product. In this paper, conventional biodiesel production with first generation feedstock, using chemical catalysts and solvent-extraction, is compared to new technologies with an emphasis on using microalgae, immobilized lipase, and SC-CO2 as an extraction solvent and reaction media.

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“…Immobilized lipases on inert supports have advantages over the conventional chemicals catalysts, showing higher selectivity and activity in the operational conditions, being easily recovered of reaction medium and having lesser cost of the purification stage of products [16]. Several applications of immobilized lipases by reversible or irreversible immobilizations are reported, such as the production of long-chain polyunsaturated fatty acids (PUFAs), especially docosahexaenoic acid (DHA, C22:6n-3) and eicosapentaenoic acid (EPA, C20:5n-3) [17]; hydrolysis of vegetable oils [18][19][20]; regio and enantioselective reactions [21][22][23][24]; and ester production [25][26][27][28]. Other immobilization methods have been studied, as the immobilization of whole cell [29] and to improve the proprieties and efficiency of enzyme immobilization, some factors have been tested to optimize this process [30,31].…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic adsorption in ionic medium improves the catalytic properties of lipases applied in the triacylglycerol hydrolysis by synergism

Quilles

Ferrarezi

Borges

et al. 2016

Bioprocess Biosyst Eng

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It is known that lipases may have their catalytic properties improved by the action of some salts or by the adsorption on hydrophobic supports. However, what we present in this work is more than that: we evaluate the combination of these two factors of hyperactivation of lipases from Acremonium-like ROG 2.1.9, a study that has not been done so far. This work proves that a synergistic effect occurs when the lipases are immobilized on hydrophobic supports at the presence of sodium chloride and are applied in triacylglycerol hydrolysis. This assay made it possible to achieve the highest hyperactivation of 500 % with the lipases immobilized on Phenyl-Sepharose and applied with 0.1 M of sodium chloride. Besides this positive effect on enzyme activity, the use of these two factors led to the thermal stability increasing of the immobilized lipases. For this derivative, the recovered activity was approximately 85 % after 6 h incubated at 55 °C and 1.0 M of the sodium chloride against 50 % of the same derivative without this salt. Furthermore, others assays were performed to prove the evidences about the synergistic effect, showing a promising method to improve the catalytic properties of the lipases from Acremonium-like ROG 2.1.9.

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Design an immobilized lipase enzyme for biodiesel production

Cited by 15 publications

References 29 publications

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

A Review of Enzymatic Transesterification of Microalgal Oil-Based Biodiesel Using Supercritical Technology

Hydrophobic adsorption in ionic medium improves the catalytic properties of lipases applied in the triacylglycerol hydrolysis by synergism

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