Design and application of oligonucleotide probes for fluorescent in situ identification of the filamentous bacterial morphotype Nostocoida limicola in activated sludge

Abstract

ErratumDesign and application of oligonucleotide probes for fluorescent in situ identification of the filamentous bacterial morphotype Nostocoida limicola in activated sludge Environmental Microbiology (2002) 4(2), 132

Identity and ecophysiology of filamentous bacteria in activated sludge

“…All isolates had the morphology described for N. limicola type I. Probes have been developed for some of the isolates (Liu & Seviour, 2001). The T. flocculiformis isolates have been characterized in pure culture, and growth on glucose produced lactate, acetate (oxically) and also ethanol and formate anoxically (Liu et al , 2002).…”

“…Several isolates exist (Blackall et al , 2000). One FISH probe is available for the actinobacterial morhotype N. limicola II (Liu & Seviour, 2001), although several separate species are targeted by it (Seviour et al , 2006, 2008). This filament is often present, but in low quantities in activated sludge systems, and suggestions have been made that it may, on rare occasions, participate in foaming incidents (Wanner, 1994).…”

“…All isolates affiliated to the genus Isosphaera (family Planctomycetaceae ), filamentous bacteria originally isolated from hotsprings (Liu et al , 2001). FISH probes were designed to target these isolates and enabled a differentiation between otherwise unrecognized bacteria with an indistinguishable morphology, but different phylogenetic affiliations (Liu & Seviour, 2001). No pure culture investigation of the isolates has been performed and no ecophysiological data exist.…”

Design and application of two oligonucleotide probes for the identification of Geodermatophilaceae strains using fluorescence in situ hybridization (FISH)

“…Probe binding profiles were performed using hybridization buffers containing 0% to 60% formamide at 5% increments on fixed type strains of species (Manz et al ., 1992; Liu and Seviour, 2001). The criteria of Weller et al .…”

“…The criteria of Weller et al . (2000) were used to determine the formamide concentrations for stringency of all the probes described in this study (Liu et al ., 2001). Optimal hybridization conditions were established by monitoring the hybridization of tested bacteria (both target and non‐target bacteria).…”