Design and Assessment of Anti-Biofilm Peptides: Steps Toward Clinical Application

Dostert, Melanie; Belanger, Corrie R.; Hancock, Robert E. W.

doi:10.1159/000491497

Cited by 93 publications

(86 citation statements)

References 72 publications

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“…This is similar to LL-37 which could also inhibit P. aeruginosa biofilm formation at sub-MIC [ 31 ]. The inhibition of biofilm by AMPs has been shown to be the result of stimulating twitching motility, influencing quorum sensing or degrading signaling molecules, such as ppGpp [ 32 , 33 ], or decreasing the expression of polysaccharide-intercellular adhesin genes [ 34 ]. The mechanism of action of melimine and Mel4 inhibiting biofilms of P. aeruginosa appeared to be very similar to their mode of action on P. aeruginosa cells in suspension [ 20 ], that is disrupting cell membranes resulting in release in intracellular contents.…”

Section: Discussionmentioning

confidence: 99%

Activity of Antimicrobial Peptides and Ciprofloxacin against Pseudomonas aeruginosa Biofilms

2020

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Pseudomonas aeruginosa is increasingly resistant to conventional antibiotics, which can be compounded by the formation of biofilms on surfaces conferring additional resistance. P. aeruginosa was grown in sub-inhibitory concentrations of the antimicrobial peptides (AMPs) melimine and Mel4 or ciprofloxacin for 30 consecutive days to induce the development of resistance. Antibiofilm effect of AMPs and ciprofloxacin was evaluated using crystal violet and live/dead staining with confocal microscopy. Effect on the cell membrane of biofilm cells was evaluated using DiSC(3)-5 dye and release of intracellular ATP and DNA/RNA. The minimum inhibitory concentration (MIC) of ciprofloxacin increased 64-fold after 30 passages, but did not increase for melimine or Mel4. Ciprofloxacin could not inhibit biofilm formation of resistant cells at 4× MIC, but both AMPs reduced biofilms by >75% at 1× MIC. At 1× MIC, only the combination of either AMP with ciprofloxacin was able to significantly disrupt pre-formed biofilms (≥61%; p < 0.001). Only AMPs depolarized the cell membranes of biofilm cells at 1× MIC. At 1× MIC either AMP with ciprofloxacin released a significant amount of ATP (p < 0.04), but did not release DNA/RNA. AMPs do not easily induce resistance in P. aeruginosa and can be used in combination with ciprofloxacin to treat biofilm.

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Section: Discussionmentioning

confidence: 99%

Activity of Antimicrobial Peptides and Ciprofloxacin against Pseudomonas aeruginosa Biofilms

2020

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“…Moreover, persister cells existing within a biofilm are metabolically inactive, so they pose a serious challenge for conventional antibiotics (Jorge et al, 2012;Shahrour et al, 2019). For a little more than a decade, AMPs have been acknowledged as a promising source of antibiofilm agents as well as of antibacterials, although only a small fraction of identified AMPs have been adequately studied in this particular aspect to date (Dostert et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Caprine Bactenecins as Promising Tools for Developing New Antimicrobial and Antitumor Drugs

Копейкин

Zharkova

Kolobov

et al. 2020

Front. Cell. Infect. Microbiol.

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initiate apoptosis in target cells, though its action was slower than that of the native ChBac3.4. Its antitumor effectiveness was successfully verified in vivo in a murine Ehrlich ascites carcinoma model. The obtained results demonstrate the potential of structural modification to manage caprine bactenecins' selectivity and activity spectrum and confirm that they are promising prototypes for antimicrobial and anticancer drugs design.

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“…Tyrothricin, a nonribosomally produced antimicrobial peptide (AMP) complex produced by the soil‐bacterium Brevibacillus parabrevis , was the first antibiotic preparation to be used in clinical practices (topical applications) but was soon replaced by penicillin . With pathogens displaying resistance to many of the existing drugs, AMPs and specifically nonribosomally produced AMPs are now reconsidered as potential antibiotics because of their broad spectrum of activity against bacteria, fungi, parasites, and certain viruses . Furthermore, the rapid antimicrobial action of AMPs and their ability to affect multiple targets decrease the likelihood of resistance developing against them …”

Section: Introductionmentioning

confidence: 99%

Tyrocidine A interactions with saccharides investigated by CD and NMR spectroscopies

Juhl

Rensburg

Bossis

et al. 2019

Journal of Peptide Science

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Tyrocidines are a family of cyclic decapeptides produced by the soil bacterium, Brevibacillus parabrevis. These antibiotic peptides can be used to prevent infections in agriculture and food industry but also to prepare antimicrobial lozenges, creams, and dressings for medical applications. It has been observed that the tyrocidines interact with saccharides such as cellulose from their soil environment, as well as sugars in culture media and glycans in fungal cell walls. Here, we investigated the interactions of tyrocidines with glucose, sucrose, and cellotetraose (as cellulose model) in a quantitative fashion utilising CD and NMR spectroscopy. The CD and NMR spectra of tyrocidine A (TrcA) were analysed as a function of solvent composition, and the spectral properties agree with the formation of oligomeric structures that are governed by β-sheet secondary structures once the acetonitrile content of the solvent is increased. Saccharides seem to also induce TrcA spectral changes reverting those induced by organic solvents. The CD spectral changes of TrcA in the presence of glucose agree with new ordered H-bonding, possibly β-sheet structures. The amides involved in intramolecular H-bonding remained largely unaffected by the environmental changes. In contrast, amides exposed to the exterior and/or involved in TrcA intermolecular association show the largest 1 H chemical shift changes. CD and NMR spectroscopic investigations correlated well with TrcAglucose interactions characterized by a dissociation constant around 200 μM. Interestingly, the association of cellotetraose corresponds closely to the additive effect from four glucose moieties, while a much higher dissociation constant was observed for sucrose. Similar trends to TrcA for binding to the three saccharides were observed for the analogous tyrocidines, tyrocidine B, and tyrocidine C. These results therefore indicate that the tyrocidine interactions with the glucose monosaccharide unit are fairly specific and reversible.

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Design and Assessment of Anti-Biofilm Peptides: Steps Toward Clinical Application

Cited by 93 publications

References 72 publications

Activity of Antimicrobial Peptides and Ciprofloxacin against Pseudomonas aeruginosa Biofilms

Activity of Antimicrobial Peptides and Ciprofloxacin against Pseudomonas aeruginosa Biofilms

Caprine Bactenecins as Promising Tools for Developing New Antimicrobial and Antitumor Drugs

Tyrocidine A interactions with saccharides investigated by CD and NMR spectroscopies

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