Design and Characterization of a Novel Blood Collection and Transportation Device for Proteomic Applications

Kaiser, Nathan K.; Steers, Maximillian; Nichols, Charles M.; Mellert, Hestia; Pestano, Gary A.

doi:10.3390/diagnostics10121032

Cited by 8 publications

(8 citation statements)

References 41 publications

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“…A lateral flow blood collection device (BCD) was fabricated to separate plasma while maintaining protein integrity at ambient storage conditions for proteomic applications. 110 The device comprises a spreading mesh, rectangular strip (85 mm Â 17 mm) of glass fiber membrane (Whatman LF1), and desiccant housed within a plastic casing (Figure 10a). The minimum sample input volume stock and accommodates up to four drops of blood.…”

Section: Passive Lateral Flow Plasma Separation Devices For Dried Pla...mentioning

confidence: 99%

“…Integrating plasma separation directly into LFTs while maintaining automated manufacturing methods could expand testing capabilities at the point‐of‐care. A lateral flow blood collection device (BCD) was fabricated to separate plasma while maintaining protein integrity at ambient storage conditions for proteomic applications 110 . The device comprises a spreading mesh, rectangular strip (85 mm × 17 mm) of glass fiber membrane (Whatman LF1), and desiccant housed within a plastic casing (Figure 10a).…”

Section: Separation Of Cellular and Liquid Components Of Whole Blood ...mentioning

confidence: 99%

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Microsampling tools for collecting, processing, and storing blood at the point‐of‐care

Baillargeon

Mace

2022

Bioengineering & Transla Med

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In the wake of the COVID-19 global pandemic, self-administered microsampling tools have reemerged as an effective means to maintain routine healthcare assessments without inundating hospitals or clinics. Finger-stick collection of blood is easily performed at home, in the workplace, or at the point-of-care, obviating the need for a trained phlebotomist. While the initial collection of blood is facile, the diagnostic or clinical utility of the sample is dependent on how the sample is processed and stored prior to transport to an analytical laboratory. The past decade has seen incredible innovation for the development of new materials and technologies to collect low-volume samples of blood with excellent precision that operate independently of the hematocrit effect. The final application of that blood (i.e., the test to be performed) ultimately dictates the collection and storage approach as certain materials or chemical reagents can render a sample diagnostically useless. Consequently, there is not a single microsampling tool that is capable of addressing every clinical need at this time. In this review, we highlight technologies designed for patient-centric microsampling blood at the point-of-care and discuss their utility for quantitative sampling as a function of collection material and technique. In addition to surveying methods for collecting and storing whole blood, we emphasize the need for direct separation of the cellular and liquid components of blood to produce cell-free plasma to expand clinical utility. Integrating advanced functionality while maintaining simple user operation presents a viable means of revolutionizing self-administered microsampling, establishing new avenues for innovation in materials science, and expanding access to healthcare.

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Section: Passive Lateral Flow Plasma Separation Devices For Dried Pla...mentioning

confidence: 99%

Section: Separation Of Cellular and Liquid Components Of Whole Blood ...mentioning

confidence: 99%

Microsampling tools for collecting, processing, and storing blood at the point‐of‐care

Baillargeon

Mace

2022

Bioengineering & Transla Med

View full text Add to dashboard Cite

show abstract

“…Integrating plasma separation directly into LFTs while maintaining automated manufacturing methods could expand testing capabilities at the point-of-care. A lateral flow blood collection device (BCD) was fabricated to separate plasma while maintaining protein integrity at ambient storage conditions for proteomic applications [110]. The device comprises a spreading mesh, rectangular strip (85 mm x 17 mm) of glass fiber membrane (Whatman LF1), and desiccant housed within a plastic casing (Figure 10A).…”

Section: Passive Lateral Flow Plasma Separation Devices For Dried Pla...mentioning

confidence: 99%

Microsampling tools for collecting, processing, and storing blood at the point-of-care

Baillargeon

Mace

2022

Preprint

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In the wake of the COVID-19 global pandemic, self-administered microsampling tools have reemerged as an effective means to maintain routine healthcare assessments without inundating hospitals or clinics. Fingerstick collection of blood is easily performed at home, in the workplace, or at the point-of-care, obviating the need for a trained phlebotomist. While the initial collection of blood is facile, the diagnostic or clinical utility of the sample is dependent on how the sample is processed and stored prior to transport to an analytical laboratory. The past decade has seen incredible innovation for the development of new materials and technologies to collect low-volume samples of blood with excellent precision that operate independently of the hematocrit effect. The final application of that blood (i.e., the test to be performed) ultimately dictates the collection and storage approach as certain materials or chemical reagents can render a sample diagnostically useless. Consequently, there is not a single microsampling tool that is capable of addressing every clinical need at this time. In this review, we highlight technologies designed for patient-centric microsampling blood at the point-of-care and discuss their utility for quantitative sampling as a function of collection material and technique. In addition to surveying methods for collecting and storing whole blood, we emphasize the need for direct separation of the cellular and liquid components of blood to produce cell-free plasma to expand clinical utility. Integrating advanced functionality while maintaining simple user operation presents a viable means of revolutionizing self-administered microsampling, establishing new avenues for innovation in materials science, and expanding access to healthcare.

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“…Plasma separation cards (PSCs) utilize passive filtration and represent an excellent option for the processing and storage of plasma samples at the point-of-care due to the associated ease of collection, low cost, and ability to ship through the mail for off-site analysis . First generation PSCs utilized either vertical or lateral , flow plasma separation membranes (PSM), often paired with a porous cardstock (e.g., Whatman CF12, Ahlstrom 226, and Munktell TFN) to produce cell-free plasma. In these formats, the distribution of plasma collected by the porous material is both unrestricted and undefined, which presents two distinct challenges: (i) variable sample output volume and (ii) chromatographic effects.…”

Section: Introductionmentioning

confidence: 99%

Direct Processing and Storage of Cell-Free Plasma Using Dried Plasma Spot Cards

et al. 2022

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Plasma separation cards represent a viable approach for expanding testing capabilities away from clinical settings by generating cell-free plasma with minimal user intervention. These devices typically comprise a basic structure of the plasma separation membrane, unconstrained porous collection pad, and utilize either (i) lateral or (ii) vertical fluidic pathways for separating plasma. Unfortunately, these configurations are highly susceptible to (i) inconsistent sampling volume due to differences in the patient hematocrit or (ii) severe contamination due to leakage of red blood cells or release of hemoglobin (i.e., hemolysis). Herein, we combine the enhanced sampling of our previously reported patterned dried blood spot cards with an assembly of porous separation materials to produce a patterned dried plasma spot card for direct processing and storage of cell-free plasma. Linking both vertical separation and lateral distribution of plasma yields discrete plasma collection zones that are spatially protected from potential contamination due to hemolysis and an inlet zone enriched with blood cells for additional testing. We evaluate the versatility of this card by quantitation of three classes of analytes and techniques including (i) the soluble transferrin receptor by enzyme-linked immunosorbent assay, (ii) potassium by inductively coupled plasma atomic emission spectroscopy, and (iii) 18S rRNA by reverse transcriptase quantitative polymerase chain reaction. We achieve quantitative recovery of each class of analyte with no statistically significant difference between dried and liquid reference samples. We anticipate that this sampling approach can be applied broadly to improve access to critical blood testing in resource-limited settings or at the point-of-care.

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Design and Characterization of a Novel Blood Collection and Transportation Device for Proteomic Applications

Cited by 8 publications

References 41 publications

Microsampling tools for collecting, processing, and storing blood at the point‐of‐care

Microsampling tools for collecting, processing, and storing blood at the point‐of‐care

Microsampling tools for collecting, processing, and storing blood at the point-of-care

Direct Processing and Storage of Cell-Free Plasma Using Dried Plasma Spot Cards

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