Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP

Feng, Shilun; Dong, Tao

doi:10.1109/memea.2014.6860076

Cited by 2 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biosensors have broad applications in drug discovery, medical diagnostics [1,2], environmental monitoring and food safety [3]. The concentration of specific analytes and their reaction kinetics can be identified in biosensors [4,5]. Biosensors using microdroplets have offered reconfigurability and flexibility and limited contamination during sample preparation and analysis [4].…”

Section: Introductionmentioning

confidence: 99%

Droplets for Sampling and Transport of Chemical Signals in Biosensing: A Review

2019

View full text Add to dashboard Cite

The chemical, temporal, and spatial resolution of chemical signals that are sampled and transported with continuous flow is limited because of Taylor dispersion. Droplets have been used to solve this problem by digitizing chemical signals into discrete segments that can be transported for a long distance or a long time without loss of chemical, temporal or spatial precision. In this review, we describe Taylor dispersion, sampling theory, and Laplace pressure, and give examples of sampling probes that have used droplets to sample or/and transport fluid from a continuous medium, such as cell culture or nerve tissue, for external analysis. The examples are categorized, as follows: (1) Aqueous-phase sampling with downstream droplet formation; (2) preformed droplets for sampling; and (3) droplets formed near the analyte source. Finally, strategies for downstream sample recovery for conventional analysis are described.

show abstract

Section: Introductionmentioning

confidence: 99%

Droplets for Sampling and Transport of Chemical Signals in Biosensing: A Review

2019

View full text Add to dashboard Cite

show abstract

“…Microfluidic devices are powerful tools for analytical chemistry, biology, diagnostics, and biomedical research. Several microfluidic devices have been developed to detect adenosine triphosphate (ATP) [1,2], nitrite [3], and bacteria [4] for Urinary tract infection detection [5]. Microfluidic devices can be miniaturized and integrated, thereby affording advantages such as easy handling, portability, and short operating time, low cost, reduced consumption of sample and reagents, and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Development of an Internet of Things Based Electrochemical Microfluidic System for Free Calcium Detection

Yuan¹,

Feng

Alahi

et al. 2018

Applied Sciences

Self Cite

View full text Add to dashboard Cite

This paper represents the design, fabrication, and implementation of an Internet of Things (IoT)-based electrochemical microfluidic system for free calcium concentration detection with a 3D printing technique. Free calcium solutions with desired concentrations between 0 and 40 µM can be obtained. The solutions were used to calibrate the system by using an impedance analyzer for monitoring the impedance change to determine the operating frequency. Continuously, an IoT enabled point of care device was used for real-time detection and to send signals to the cloud for sharing. The relationship between the concentration and reactance are y = −1.3812Lgx + 0.9809 at a wavelength of 450 Hz, with an R 2 of 0.9719. We measured the calcium concentration changing from 39.8 µM to 1.35 µM (nearly real-time) by the PoC device and showed the concentration changes resulting with time on the cell phone app. The results depicted in this paper provide a strong platform for the precise and real-time monitoring of different biomedical samples. Appl. Sci. 2018, 8, 1357 2 of 15 and sophisticated laboratory systems into friendly home-use devices [15][16][17]. Different applications had been achieved, especially in the fields of clinical diagnostic and environmental monitoring. This is because of their low cost, ease of use, reliability, and portability [18][19][20][21][22][23][24]. Moreover, some PoC devices can carry out real time testing which can produce results rapidly. By this way, patient-care can be improved with less costs and treatment can start more quickly [25,26].Wireless transmissions and cloud servers enable transferring and sharing the measured data while being connected with the monitored patient, which enhances the functionalities of the sensing systems [27,28]. It is powered by the Internet of Things (IoT) concept, where all the connected objects that surround us can exchange data over a network. IoT-enabled smart devices and health services can reduce the diagnostic cost, provide real-time information to the house physician, and provide important health information without any mobility of the patient, therefore increasing their quality of life and the efficiency [26,29].In this paper, we devised the use of silicon-based sensors integrated into the microfluidics system with 3D printing. IoT techniques were used to store and transmit the data to end-users. One of the prominent biomedical applications was where free calcium iron solutions were measured in different concentrations. Commercial calcium buffer solutions were used to calibrate the system. The determination of free calcium is very important in human and veterinary pathology and in dairy chemistry [30][31][32].

show abstract

Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP

Cited by 2 publications

References 13 publications

Droplets for Sampling and Transport of Chemical Signals in Biosensing: A Review

Droplets for Sampling and Transport of Chemical Signals in Biosensing: A Review

Development of an Internet of Things Based Electrochemical Microfluidic System for Free Calcium Detection

Contact Info

Product

Resources

About