Design and characterization of ellagic acid-loaded zein nanoparticles and their effect on the antioxidant and antibacterial activities

Tavares, Walter de Souza; Pena, Gabriela Ribeiro; Martín-Pastor, Manuel; Sousa, Francisco Fábio Oliveira de

doi:10.1016/j.molliq.2021.116915

Cited by 38 publications

(23 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nanoparticles were prepared by nanoprecipitation, according to our previous studies [ 15 , 16 ]. This method allows obtaining small and homogeneous droplets by increasing the surface area through the disruption in the semipolar solvent containing the polymer and the aqueous interphase.…”

Section: Methodsmentioning

confidence: 99%

“…Corn protein zein, a prolamin found in the endosperm of maize ( Zea mays ), is a biodegradable and biocompatible biopolymer able to entrap and deliver therapeutic molecules [ 12 , 13 , 14 , 15 , 16 , 17 ]. Due to these interesting properties zein is an abundantly renewable resource that has long been applied in food and pharmaceutical industries [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…The incorporation of charged surfactants can effectively improve their stability. Recently, our group has demonstrated its ability to improve the antimicrobial and antibiofilm activities of therapeutic molecules [ 15 , 16 , 19 ]. Given the above background the aim of this work was to prepare and characterize a green antimicrobial system based on zein nanoparticles and cationic amino acid surfactants.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

et al. 2023

View full text Add to dashboard Cite

Although cationic surfactants have a remarkable antimicrobial activity, they present an intrinsic toxicity that discourages their usage. In this work novel zein nanoparticles loaded with arginine-phenylalanine-based surfactants are presented. The nanoparticles were loaded with two single polar head (LAM and PNHC12) and two with double amino acid polar head surfactants, arginine-phenylalanine (C12PAM, PANHC12). The formulations were characterized and their stability checked up to 365 days. Furthermore, the antimicrobial and hemolytic activities were investigated. Finally, NMR and molecular docking studies were carried out to elucidate the possible interaction mechanisms of surfactant-zein. The nanoparticles were obtained with satisfactory size, zeta potential and dispersibility. The surfactants containing arginine-phenylalanine residues were found to be more stable. The nanoencapsulation maintained the antimicrobial activities unaltered in comparison to the surfactants’ solutions. These results are in agreement with the NMR and docking findings, suggesting that zein interacts with the surfactants by the aromatic rings of phenylalanine. As a result, the cationic charges and part of the aliphatic chains are freely available to attack the bacteria and fungi, while not available to disrupt the cellular membranes. This approach opens new possibilities for using cationic surfactants and benefits from their extraordinary antimicrobial responses for several applications.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Zein nanoparticles also showed an enhanced antioxidant activity compared to their natural form. 51 The reason could be that during the formation of nanoparticles, the protein structure was opened up, and antioxidant amino acids such as cysteine, histidine, alanine, etc. , 52 were exposed from the interior of the protein.…”

Section: Resultsmentioning

confidence: 99%

Soy protein nanoparticles prepared by enzymatic cross-linking with enhanced emulsion stability

Sun

Wei

et al. 2023

Soft Matter

View full text Add to dashboard Cite

show abstract

“…Nanoprecipitation was successfully used by de Souza Tavares et al [ 130 ] who nanoencapsulated ellagic acid, a compound with antioxidant and antimicrobial activities, into zein nanoparticles. They obtained spherical, non-aggregated, smooth-surface particles under 370 nm in diameter with relevant inhibitory and bactericide activity against S. aureus and P. aeruginosa .…”

Section: Microparticles and Nanoparticles Productionmentioning

confidence: 99%

Zein Microparticles and Nanoparticles as Drug Delivery Systems

Marco

2022

Polymers

View full text Add to dashboard Cite

Zein is a natural, biocompatible, and biodegradable polymer widely used in the pharmaceutical, biomedical, and packaging fields because of its low water vapor permeability, antibacterial activity, and hydrophobicity. It is a vegetal protein extracted from renewable resources (it is the major storage protein from corn). There has been growing attention to producing zein-based drug delivery systems in the recent years. Being a hydrophobic biopolymer, it is used in the controlled and targeted delivery of active principles. This review examines the present-day landscape of zein-based microparticles and nanoparticles, focusing on the different techniques used to obtain particles, the optimization of process parameters, advantages, disadvantages, and final applications.

show abstract

Design and characterization of ellagic acid-loaded zein nanoparticles and their effect on the antioxidant and antibacterial activities

Cited by 38 publications

References 43 publications

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

Soy protein nanoparticles prepared by enzymatic cross-linking with enhanced emulsion stability

Zein Microparticles and Nanoparticles as Drug Delivery Systems

Contact Info

Product

Resources

About