Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

Recent advances in biosynthesis of fatty acids derived products in Saccharomyces cerevisiae via enhanced supply of precursor metabolites

“…Additional deletion of CIT2 or MLS1 in the ∆adh1-∆adh4 strain impaired acetyl-CoA biosynthesis and accordingly decreased n-butanol production [43]. The unexpected results might be caused by the impaired C 2 metabolism, since the decreased production of acetyl-CoA derived products was accompanied with the accumulation of acetate.…”

“…3, acetaldehyde is the branch point to control the flux distribution between ethanol and acetyl-CoA, and alcohol dehydrogenases (ADHs) were chosen as the metabolic engineering targets. By the deletion of major ADHs in the cytosol (∆adh1-∆adh4), acetyl-CoA level was increased around 2-fold and the production of n-butanol, an important biofuel molecule derived from acetyl-CoA, was increased more than 4-fold [43]. In another study, 1.9-fold improvement in fatty acids production was achieved by knocking out ADH1 in a fatty acids producing host [42].…”

“…3) were introduced to boost the availability of acetyl-CoA in yeast [34,67]. Notably, improved production of amorphadiene [59], α-santalene [6], PHB [33,34], n-butanol [36,43], 3-HP [75], and fatty acids [67] were achieved by engineering the acetyl-CoA pool in S. cerevisiae. Considering the significance of acetyl-CoA in cellular metabolism, nature has evolved various routes to synthesize acetyl-CoA under different conditions.…”

Metabolic Engineering for Advanced Biofuels Production and Recent Advances Toward Commercialization

“…[20] However, the highest n-butanol titers (242.8 mg L À1 ) have been reported in the strain that utilizes a native keto acid-like pathway and contains a double deletion of ilv2 and adh1 to direct cytosolic pyruvate to mitochondrial threonine metabolism. [21] The first demonstration of microbial production of isobutanol in yeast was achieved by overexpressing genes involved in valine metabolism (ilv2, ilv5, and ilv3).…”

Genetic Engineering In BioButanol Production And Tolerance

“…The optimal 1-butanol and isobutanol production was approximately matched with the theoretical production of butanol product. The maxima was 242.8mg/L from glucose by deleting gene ∆adh1, ∆ilv2 of YSG52 strain and 92mg/L from glycine as a single protein source by using novel pathway by converting glycine into glyoxylate further β-ethylmalate then α-ketovalerate into butanol by following Ehlich pathway 62 . The maxima for optimum production of isobutanol was 1620mg/L in a YPH499 strain by using full cytoplasmic pathway with concomitant mitochondrial gene ILv2,ILV2, ILV2∆54, ILV3∆41, ILV5∆47,ADH6, MAE1 Lactococcus lactis gene kivD 63 .…”