2011
DOI: 10.1017/s1431927610094341
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Design and Demonstration of a Microbiaxial Optomechanical Device for Multiscale Characterization of Soft Biological Tissues with Two-Photon Microscopy

Abstract: The biomechanical response of tissues serves as a valuable marker in the prediction of disease and in understanding the related behavior of the body under various disease and age states. Alterations in the macroscopic biomechanical response of diseased tissues are well documented; however, a thorough understanding of the microstructural events that lead to these changes is poorly understood. In this article we introduce a novel microbiaxial optomechanical device that allows two-photon imaging techniques to be … Show more

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Cited by 26 publications
(37 citation statements)
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“…Furthermore, as part of the study of the tissue engineered scaffolds, our future work will also involve the biomechanical characterization of electrospun sheets seeded with SMCs and treated with exogenous TGFβ2 at different concentrations. The biomechanical response will be measured using a microbiaxial optomechanical device (MOD) designed in our laboratory to simultaneously measure the macroscopic and microstructural properties of planar and tubular vascular tissues [22, 59, 60]. …”
Section: Discussionmentioning
confidence: 99%
“…Furthermore, as part of the study of the tissue engineered scaffolds, our future work will also involve the biomechanical characterization of electrospun sheets seeded with SMCs and treated with exogenous TGFβ2 at different concentrations. The biomechanical response will be measured using a microbiaxial optomechanical device (MOD) designed in our laboratory to simultaneously measure the macroscopic and microstructural properties of planar and tubular vascular tissues [22, 59, 60]. …”
Section: Discussionmentioning
confidence: 99%
“…Referring to the presented results [12] and interpreting fiber reorientations under increasing circumferential stretches (λ = 1 to λ = 1.7) at a given axial stretch (λ = 1.5), which is a loading scenario kinematically equivalent to inflation at constant axial stretch, we note that fibers showed limited reorientations (their rotation being 30% lower than predicted by the affine kinematics). To our best knowledge, only Keyes et al (2011) [30] obtained results with different trends although applying an analogous mechanical protocol.…”
Section: Stack Of Imagesmentioning
confidence: 96%
“…In agreement with well-established tension-inflation protocols for arteries [26,30], two loading scenarii were considered, namely axial tension under constant imposed pressure and inflation under constant axial stretch. Four carotids were used in the present study, carotid samples S1 and S2 were tested under both loading scenarii, while carotid samples S3 and S4 were tested under only one loading scenario, i.e.…”
Section: Loading Scenario and Data Acquisitionmentioning
confidence: 99%
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“…Autofluorescence emission from elastin is collected through another bandpass filter (460/80). Further detail on the AIM and the integration of our mechanical device into the AIM can be found in Keyes et al, 2011a. As obtaining a single image stack of an aorta took approximately 10 minutes, each vessel was slowly displaced axially to the physiologic strain used in the mechanical test and held for 3 min to eliminate any viscoelastic effects. Three image stacks were taken for each specimen, all at the physiologic strain with pressurizations of 07 2, 507 2, 1007 2 mmHg, each held for a time after being inflated again to eliminate any viscoelastic effects.…”
Section: Multiphoton Imagingmentioning
confidence: 99%