Design and development of nanostructured co delivery of artemisinin and chrysin for targeting hTERT gene expression in breast cancer cell line: possible clinical application in cancer treatment

Abstract

Background: Breast cancer is one of the most significant causes of female cancer death worldwide. To explore the possibility of a novel chemo-preventive strategy for improving breast cancer treatment, the anticancer effects of a combination two natural compounds, Artemisinin (Art) and Chrysin (Chr), against T47D breast cancer cells were investigated.Methods: For this purpose, Art and Chr were co-encapsulated in PEGylated PLGA nanoparticles (NPs) and evaluated for their therapeutic efficacy. The morphology and dynamic light scattering (DLS) analyses were carried out to optimize the Nano formulations. Drug release study was performed using the dialysis method and then the cytotoxic and inhibitory effect of individual and combined drugs on the expression level of hTERT in the T47D breast cell line was evaluated using MTT assay and qPCR, respectively. Results: The results showed that pure drugs and formulations exhibited dose-dependent cytotoxicity against T47D cells and especially, Art/Chr–PLGA/PEG NPs had a more synergistic anti-proliferative effect and significantly arrested the growth of cancer cells than the other groups. Real-time PCR results revealed that Art, Chr and combination of Art–Chr in pure and encapsulated forms inhibited hTERT gene expression. Conclusions: It was found that Art–Chr–PLGA/PEG NPs relative to pure combination could further decline hTERT expression in all concentrations. Our study demonstrated that Art–Chr–PLGA/PEG NPs based combinational therapy holds promising potential for the treatment of breast cancer.

Fabrication of magnetic niosomal platform for delivery of resveratrol: potential anticancer activity against human pancreatic cancer Capan-1 cell

“…Numerous reports have revealed that the surface charge of NPs plays a crucial role in their interaction with cells and subsequent internalization. Positively charged NPs often exhibit higher cellular internalization compared to neutral or negatively charged counterparts, and this phenomenon is attributed to several factors including enhanced cell membrane penetration, cellular uptake pathways and influence on intracellular trafficking [ 41 , 42 ]. In current work, the surface charge of the prepared RSV-MNIONPs was determined using the DLS technique as the zeta potential was − 12.06 ± 3.4 and polydispersity index (PDI) was 0.127 ± 0.034.…”

Therapeutic potential of chrysin nanoparticle-mediation inhibition of succinate dehydrogenase and ubiquinone oxidoreductase in pancreatic and lung adenocarcinoma

“…Phytochemicals such as chrysin have various drawbacks and restrictions that impede their biological function; however, improvements in nanotechnology have considerably mitigated these drawbacks. Short half-life, limited solubility, poor biological availability, short circulatory stability length, and fast metabolism and degradation are only a few of these drawbacks [68].…”