Design and development of solid lipid nanoparticles for topical delivery of an anti-fungal agent

In vitro anti-cancer effects of artemisone nano-vesicular formulations on melanoma cells

“…An aqueous phase was prepared by dissolving PVA (0.25%) in water and was heated to the same temperature as the organic phase. The organic phase was added dropwise by means of a hypodermic needle to the pre-heated aqueous phase with continuous stirring [26]. The mixture was allowed to cool and was sonicated (Hielscher UP 200ST, South Africa) for varying periods of time until nano-vesicles of the desired size were obtained.…”

In-vitro efficacies of topical microemulsions of clotrimazole and ketoconazole; and in-vivo performance of clotrimazole microemulsion

“…The density of inoculums was adjusted by measuring the optical density of the microbial suspension. About 75 µl Candida albicans culture (cfu/ml 10 8 ) was injected subcutaneously and 25µl candida culture was applied topically on the exposed skin[24][25][26]. Symptoms of infection were observed in 24-48 hours.…”

Miconazole-loaded solid lipid nanoparticles: formulation and evaluation of a novel formula with high bioavailability and antifungal activity

“…[14][15][16] These systems have been shown to enhance the antifungal activity of miconazole. 4 To date, 17,18 Solid lipid nanoparticles (SLNs) were developed at the beginning of the 1990s as an alternative particulate delivery system to liposomes, emulsions, microparticles, and polymeric nanoparticulate systems. SLNs combine the advantages of those systems, such as controlled release, biodegradability, biocompatibility, and the protection of the incorporated drug against chemical degradation.…”

“…1,2 Miconazole is a weak base and shows poor aqueous solubility (,1 µg/mL); as a result, a reduction in its therapeutic efficacy has been reported. 3,4 Miconazole has a dual mechanism of action: the inhibition of ergosterol biosynthesis and the inhibition of peroxidases, which causes the accumulation of peroxide within the cell, leading to cell death. 5,6 This antifungal drug is available in different dosage forms, including topical, oral, and parenteral, depending on the type and severity of infection.…”