Design and evaluation of a multifunctional thermosensitive poloxamer-chitosan-hyaluronic acid gel for the treatment of skin burns

Soriano-Ruiz, José L.; Calpena-Campmany, Ana C.; Silva-Abreu, Marcelle; Halbout-Bellowa, Lyda; Febrer, Nuria Bozal-de; Rodríguez-Lagunas, Maria J.; Clares, Beatriz

doi:10.1016/j.ijbiomac.2019.09.113

Cited by 57 publications

(33 citation statements)

References 26 publications

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“…As expected, the treatment with PLX/CS/HA did not significantly modify the Young's modulus distribution when compared with burned skin ( Figure 7B). Surprisingly, the PLX/CS/HA-V, although it has demonstrated some wound healing efficacy in previous studies [17], did not differ significantly from the untreated damaged skin ( Figure 7B). Interestingly, the treatment with PLX/CS/HA-M ( Figure 8C) partially recovers the main peak found in the normal skin ( Figure 7A) but with less frequency, as there are still some regions with high Young's modulus values.…”

Section: Atomic Force Microscopymentioning

confidence: 62%

“…Furthermore, it has also been associated with participation in wound healing, specifically in macrophage-mediated inflammatory processes as well as angiogenesis [16]. Taking all these vitamins together, we have previously developed a thermosensitive hydrogel loaded with vitamin A, D and E for the treatment of skin burns [17].…”

Section: Introductionmentioning

confidence: 99%

“…It is also remarkable to take into consideration the biological properties of biomaterials forming the drug carrier. In previous studies, our group has demonstrated the potential of a hydrogel loading antioxidant actives in wound healing, consisting of poloxamer 407 (PLX), chitosan (CS) and hyaluronic acid (HA) [17,36]. Hydrogel might absorb exudates and provide a moist environment, preventing wound dehydration [37].…”

Section: Introductionmentioning

confidence: 99%

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Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns

et al. 2020

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The main goal of this work is the study of the skin wound healing efficacy of an antioxidant cocktail consisting of vitamins A, D, E and the endogenous pineal hormone melatonin (MLT), with all of these loaded into a thermosensitive hydrogel delivery system. The resulting formulation was characterized by scanning electron microscopy. The antioxidant efficacy and microbiological activity against Gram positive and Gram negative strains were also assayed. The skin healing efficacy was tested using an in vivo model which included histological evaluation. Furthermore, atomic force microscopy was employed to evaluate the wound healing efficacy of rat skin burns through the determination of its elasticity at the nanoscale using force spectroscopy analysis. The resulting hydrogel exhibited sol state at low temperature and turned into a gel at 30 ± 0.2 °C. The hydrogel containing the antioxidant cocktail showed higher scavenging activity than the hydrogel containing vitamins or MLT, separately. The formulation showed optimal antimicrobial activity. It was comparable to a commercial reference. It was also evidenced that the hydrogel containing the antioxidant cocktail exhibited the strongest healing process in the skin burns of rats, similar to the assayed commercial reference containing silver sulfadiazine. Histological studies confirmed the observed results. Finally, atomic force microscopy demonstrated a similar distribution of Young’s modulus values between burned skin treated with the commercial reference and burned skin treated with hydrogel containing the antioxidant cocktail, and all these with healthy skin. The use of an antioxidant cocktail of vitamins and MLT might be a promising treatment for skin wounds for future clinical studies.

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Section: Atomic Force Microscopymentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns

et al. 2020

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“…Of note, several studies demonstrated local tolerance to p407 poloxamer hydrogel when tested on the skin, including the burnt skin [ 22 , 34 ]. Moreover, a number of studies used a poloxamer hydrogel as a vehicle for bioactive molecules aimed at promoting skin healing [ 21 , 34 , 35 , 36 , 37 , 38 ]. These various studies highlighted the value of using a poloxamer hydrogel as a delivery system for an active ingredient.…”

Section: Discussionmentioning

confidence: 99%

“…As an example, a poloxamer hydrogel allows the transcutaneous delivery of morphine, the analgesic effects of which were observed up to 24 h after application [ 35 ]. Furthermore, a hyaluronic-acid- and chitosan-containing p407 poloxamer hydrogel was successfully used for the topical application of vitamins (A, D and E) in a mouse burn model [ 34 ]. However, it should be noted that, in the abovementioned works, the impact of poloxamer hydrogel alone was not assessed.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Topically Testable Model of Burn Injury on Human Skin Explants

Gross-Amat

Guillen

Salmon

et al. 2020

IJMS

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Severe burn injuries remain a major health problem due to high rates of mortality, residual morbidity, and/or aesthetic damages. To find new therapies aimed at promoting a harmonious healing of skin burns, it is important to develop models which take into account the unique properties of the human skin. Based on previously described models of burn injury performed on human skin explants, we hypothesized that maintaining explants under constant tension forces would allow to more closely reproduce the pathophysiological processes of skin remodeling. We thus. Here, we set up and characterized an improved model of deep second-degree burn injury on ex vivo cultured human skin explants at air-liquid interface and maintained under conditions of constant tension forces. A spontaneous re-epithelialization of the lesion was observed 8 to 9 days post burn and was found to rely on the proliferation of basal keratinocytes at the wound edges. Collagen VII at the dermo-epidermal junction reformed along with the progression of re-epithelializatio and a synthesis of procollagen III was observed in the dermis at the wound site. These findings indicate that our model is suitable for the assessment of clinically-relevant therapies aimed at modulating the kinetics of re-epithelialization and/or the activation of fibroblasts following skin burn injuries. In this regard, we evaluated the use of a thermoreversible poloxamer hydrogel as a vehicle for topically-testable therapeutic molecules. Our data showed that, although useful for drug formulation, the p407/p188 poloxamer hydrogel induces a delay of skin re-epithelialization in humans skin explants submitted to experimental burn injury.

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Ternary Thermosensitive Hydrogel‐Encapsulated Macrolactam Heneicosapeptide Eliminates Epidermal Multidrug‐Resistant Bi‐Microbial Colonization

Zeng,

Zhang,

et al. 2024

Adv Funct Materials

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Superbug epidemic has rendered antibiotic therapeutics increasingly ineffective. Worse still, there are few applicable medication regimens for polymicrobial infections caused by two or more multidrug‐resistant bacteria. Herein, a panel of antibacterial cyclic peptides are designed and synthesized and the lead compound cyclo‐zp80r shows favorable activities against a broad spectrum of bacteria. Encouragingly, it exhibited a strong bactericidal effect against two important epidermic species Pseudomonas aeruginosa and Staphylococcus aureus for both single‐ and co‐infections. The peptide cyclo‐zp80r is proposed to destroy the membrane structures of both Gram‐negative and Gram‐positive bacteria, inducing a variety of physiological disorders. To better adapt to topical administration of this novel antibacterial agent, a hydrogel formulation consisting of poloxamer 407, poloxamer 188, and hyaluronic acid is optimized. This ternary hydrogel system is able to form in situ gel at skin temperature. Encapsulated peptide molecules are released steadily in both human skin ex vivo model and mouse wound in vivo model to treat bi‐microbial infection. This work systematically investigates the design, synthesis, antibacterial mechanism of a novel cyclic peptide, and its drug delivery strategy for topical wound infection, offering a promising therapeutics to treat multidrug‐resistant polymicrobial wound infections.

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Design and evaluation of a multifunctional thermosensitive poloxamer-chitosan-hyaluronic acid gel for the treatment of skin burns

Cited by 57 publications

References 26 publications

Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns

Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns

Characterization of a Topically Testable Model of Burn Injury on Human Skin Explants

Ternary Thermosensitive Hydrogel‐Encapsulated Macrolactam Heneicosapeptide Eliminates Epidermal Multidrug‐Resistant Bi‐Microbial Colonization

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