Design and fabrication of a hydrodynamic chromatography chip

Blom, M.T.; Chmela, E.; Gardeniers, Han; Tijssen, R.; Elwenspoek, M.; Berg, Albert van den

doi:10.1016/s0925-4005(01)00989-3

Cited by 47 publications

(51 citation statements)

References 17 publications

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“…Another drawback of silicon microchips is the poor voltage breakdown characteristics, even with the support of an insulating layer deposited inside the channels [20]. Today, silicon microchips are mostly used in hybrid structures where the channels are silicon-based and the cover plate is made of glass, a configuration that allows optical detection [21].…”

Section: Silicon Wafermentioning

confidence: 99%

“…This latter design, besides giving a welldefined sample plug, also allowed larger injection volumes. The so-called "three injection" [21,36] was constructed to suppress injection-mediated tailing. Three phases of reproducible injection with well-defined sample plug using simple injection cross were recently discussed [81].…”

Section: Microchip Layout Considerationsmentioning

confidence: 99%

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New advances in microchip fabrication for electrochromatography

Szekeres

Guttman

2005

Electrophoresis

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There is a great demand in separation technologies for faster and more effective analysis processes. Miniaturization is a suitable technique for satisfying this demand as reduction in size gives increased separation speed with higher efficiency. CEC is an electric-field-mediated separation technique where the liquid flow is generated by the electric field itself. The main advantage of using electric field over pressure for flow generation is the flat flow profile of the EOF; thus, CEC is one of the best candidates to construct a novel and high-efficiency microanalytical device. The aim of the present paper is to review the basic fabrication and bonding principles, as well as connection and system integration options for microfluidics-based electrochromatography. The physical structure and fluidic channel formation are critically evaluated, including glass microstructuring and fusion bonding. Recent developments in nanoflow measurements and the application of various flow control units are also extensively discussed.

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Section: Silicon Wafermentioning

confidence: 99%

Section: Microchip Layout Considerationsmentioning

confidence: 99%

New advances in microchip fabrication for electrochromatography

Szekeres

Guttman

2005

Electrophoresis

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“…Soon it will be possible to perform HDC size-fractionations of low Mw molecules using channels with very small hydraulic diameter [9,98] or columns packed with very small particles. Non-porous silica particles with diameter <1 μm can be easily produced [97].…”

Section: Current and Future Developmentsmentioning

confidence: 99%

Size Fractionation by Slalom Chromatography and Hydrodynamic Chromatography

Dias¹

2008

ENG

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Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques.

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“…In this case we aim at integration with an on-chip Hydrodynamic Chromatography system as described in [4]- [6]. Separation by hydrodynamic chromatography is entirely based on the flow profile: in a small channel with a pressure-driven parabolic flow, larger molecules will not be able to approach the wall as much as smaller molecules and thus will have a larger average velocity (see Fig.…”

Section: Integration With On-chip Hydrodynamic Chromatographymentioning

confidence: 99%

“…Unfortunately, detection suffered from the low signal levels generated in the small detection volume. By using a planar chip configuration as described in [4]- [6] with a depth in the micrometer range and a channel that is as wide as possible, the detection volume can be increased while maintaining the geometrical separation. For practical reasons described in [4] the width is restricted to 1 mm.…”

Section: Integration With On-chip Hydrodynamic Chromatographymentioning

confidence: 99%

A differential viscosity detector for use in miniaturized chemical separation systems

Blom

Chmela

Heyden

et al. 2005

J. Microelectromech. Syst.

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Abstract-In this paper, we present a micromachined differential viscosity detector suitable for integration into an on-chip hydrodynamic chromatography system. The general design, however, is applicable to any liquid chromatography system that is used for separation of polymers. The micromachined part of the detector consists of a fluidic Wheatstone bridge and a low hydraulic capacitance pressure sensor of which the pressure sensing is based on optical detection of a membrane deflection. The stand-alone sensor shows a resolution in specific viscosity of 3 10 3 , in which specific viscosity is defined as the increase in viscosity by a sample, relative to the baseline viscosity of a solvent.[0947]

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Design and fabrication of a hydrodynamic chromatography chip

Cited by 47 publications

References 17 publications

New advances in microchip fabrication for electrochromatography

New advances in microchip fabrication for electrochromatography

Size Fractionation by Slalom Chromatography and Hydrodynamic Chromatography

A differential viscosity detector for use in miniaturized chemical separation systems

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