Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels

Hassan, Sammer-ul; Zhang, Xunli

doi:10.3390/mi11040385

Cited by 5 publications

(8 citation statements)

References 18 publications

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“…Although controlled orientation during glycan immobilization can be easily achieved using glycan derivatives (i.e., one terminal-NH 2 group for covalent amine coupling), controlled lectin immobilization intended for creating lectin biosensors and lectin microarrays is challenging due to the greater complexity of a protein molecule compared with that of a glycan molecule. Recent developments in reagent-coating of microchips [103,104] could provide possibilities for glycan/lectin coating inside miniaturized devices via optical detection.…”

Section: Challenges For Glycan-and Lectin-based Biosensorsmentioning

confidence: 99%

Glycoprotein- and Lectin-Based Approaches for Detection of Pathogens

et al. 2020

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Infectious diseases alone are estimated to result in approximately 40% of the 50 million total annual deaths globally. The importance of basic research in the control of emerging and re-emerging diseases cannot be overemphasized. However, new nanotechnology-based methodologies exploiting unique surface-located glycoproteins or their patterns can be exploited to detect pathogens at the point of use or on-site with high specificity and sensitivity. These technologies will, therefore, affect our ability in the future to more accurately assess risk. The critical challenge is making these new methodologies cost-effective, as well as simple to use, for the diagnostics industry and public healthcare providers. Miniaturization of biochemical assays in lab-on-a-chip devices has emerged as a promising tool. Miniaturization has the potential to shape modern biotechnology and how point-of-care testing of infectious diseases will be performed by developing smart microdevices that require minute amounts of sample and reagents and are cost-effective, robust, and sensitive and specific. The current review provides a short overview of some of the futuristic approaches using simple molecular interactions between glycoproteins and glycoprotein-binding molecules for the efficient and rapid detection of various pathogens at the point of use, advancing the emerging field of glyconanodiagnostics.

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Section: Challenges For Glycan-and Lectin-based Biosensorsmentioning

confidence: 99%

Glycoprotein- and Lectin-Based Approaches for Detection of Pathogens

et al. 2020

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“…Furthermore, the presence of β-lactamase, an indicator of antimicrobial resistance [23,24] was tested by dipping nitrocefin-coated microchannels into a β-lactamase solution (Abcam, recombinant Escherichia coli β-lactamase protein) and DI water samples. Nitrocefin (0.25 mg/mL in dimethyl sulfoxide) was loaded into the microchannels and left for 30 min at room temperature.…”

Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

“…The red colour intensity increased with time (Figure 4a), and the colour intensity levels variability was found to be 5% (%RSD) between the 15 microchannels and 9% (%RSD) between the microchips (n = 3). Figure 4c shows the graph of colour intensity change at different times (3,6,12,18,24,30, 60 and 120 s) for the 15 microchannels in a single microchip. Biosensors 2020, 10, x FOR PEER REVIEW 5 of 8 Furthermore, the presence of β-lactamase, an indicator of antimicrobial resistance [23,24] was tested by dipping nitrocefin-coated microchannels into a β-lactamase solution (Abcam, recombinant Escherichia coli β-lactamase protein) and DI water samples.…”

Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

“…The red colour intensity increased with time ( Figure 4a), and the colour intensity levels variability was found to be 5% (%RSD) between the 15 microchannels and 9% (%RSD) between the microchips (n = 3). Figure 4c shows the graph of colour intensity change at different times (3,6,12,18,24,30, 60 and 120 s) for the 15 microchannels in a single microchip. A smartphone (iPhone XR) was used to capture images of the microchip under external light, which could add variations in the light signal of the different microchannels.…”

Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

“…Smartphones can have a variety of optical components, and the quality of an image can be different, which can also add variability to the colour measurements. These variations can be reduced by placing the microchips in a light-controlled box with even light or a white background in a black box and running multiple Furthermore, the presence of β-lactamase, an indicator of antimicrobial resistance [23,24] was tested by dipping nitrocefin-coated microchannels into a β-lactamase solution (Abcam, recombinant Escherichia coli β-lactamase protein) and DI water samples. Nitrocefin (0.25 mg/mL in dimethyl sulfoxide) was loaded into the microchannels and left for 30 min at room temperature.…”

Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

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Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics

Hassan

Zhang

2020

Biosensors

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Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which works on the principle of capillary-driven flow microfluidics and allows analytes' detection by multiple microchannels in a single microchip via smartphone imaging. The chip-and-dip device, fabricated with inexpensive materials, works by simply dipping the reagents-coated microchip consisting of microchannels into a fluidic sample. The sample is loaded into the microchannels via capillary action and reacts with the reagents to produce a colourimetric signal. Unlike dipstick tests, this device allows the loading of bacterial/pathogenic samples for antimicrobial testing. A single device can be coated with multiple reagents, and more analytes can be detected in one sample. This platform could be used for a wide variety of assays. Here, we show the design, fabrication and working principle of the chip-and-dip flow device along with a specific application consisting in the determination of β-lactamase activity and cortisol. The simplicity, robustness and multiplexing capability of the chip-and-dip device will allow it to be used for POC diagnostics.

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