Design and Fabrication of PDMS Microchamber for Fast Reaction Assays in Biosensing Application

Abstract

A simple design of a microchamber for use in reacting assays with limited sample availability has been proposed, analyzed using COMSOL 3.5 Multiphysics simulation and fabricated using soft-lithography technique . The design is based on differential pressure drop flow using capillary effect concept which has facilitated a number of interesting flow phenomena in micro-domains. For an average pressure drop of about 100/m in the setup, flow rates of bout 0.5 to 0.8 μl/s were obtained. The component a microchamber, three designs were tested (50, 70, 90 microns in width) to give a continuous open circuit flow. The system was designed and fabricated for continuous flow across sensing element where there is a requirement for low residence time due to fast reaction/diffusion rates.Also in this paper, is a sensitive and selective, also rapid, reliable, cost-effective, and suitable for in situ analysis polymer biosensor for new bio molecular approach for cancer and cancer related diseases detection at the early stage is demonstrated.

Capillary Driven Muilti Channels Microfluidic

“…Today, the quest for small and sensitive sensor system has increase, especially lab on chip system which recently have found increasing use in portable and personalized bio sensing [1][2][3][4]. The microfluidic based portable system show potential ability to precisely deliver samples to sensing domain [5][6][7][8][9]. Integration of microfluidic with Nano sensors allow ranges possible solutions in various applications, provide the design, fabrication and integration subsystem are compatible [10].…”

“…Within this low cost diagnosis, will have the potential to change to aware diseases in an earlier stage and if it is affected, can get treatment as soon as possible. Mixing is one of the primary limitations of microfluidics systems [1][2][3][4][5][6]. Mixing process is the fundamental for gaining speedy reactions and trustworthy conclusion.…”