Design and fabrication of reusable core–shell composite microspheres based on nanodiamond for selective enrichment of phosphopeptides

Carbon-Based Nanoparticles and Their Surface-Modified Counterparts as MALDI Matrices

“…172 MALDI analysis showed more phosphopeptide species with significantly higher signal intensities after analyte enrichment with synthesized ND composites. 172 The optical absorbance and stability of NDs make them a potential MALDI matrix that can be used alone or in combination with conventional matrices. However, in many studies such as the ones mentioned above, conventional matrices are combined with the ND−analyte complex before MALDI analysis.…”

“…172 Anchoring the NDs on the microsphere surface prevented ND agglomeration in the solution and increased their efficiency by allowing the particles to expose higher surface areas. 172 MALDI analysis showed more phosphopeptide species with significantly higher signal intensities after analyte enrichment with synthesized ND composites. 172 The optical absorbance and stability of NDs make them a potential MALDI matrix that can be used alone or in combination with conventional matrices.…”

“…The surface of the NDs provides an efficient platform to conjugate other sorbents for selective extraction of specific compounds from a complex mixture followed by immediate MALDI analysis. Jia et al grafted amine functionalized NDs on the surface of polymeric microspheres and covalently conjugated the ND’s amine group to a metal chelating agent to selectively enrich phosphopeptides in biological samples . Anchoring the NDs on the microsphere surface prevented ND agglomeration in the solution and increased their efficiency by allowing the particles to expose higher surface areas .…”

Advances in proteomics sample preparation and enrichment for phosphorylation and glycosylation analysis

“…Polyphenolic ligands containing multiple hydroxyl groups are increasingly used as linkers for IMAC materials due to their rapid coordination chelation. Various polyphenol chelating agents have been developed, including 2,3,4-trihydroxybenzaldehyde [112], tannic acid [113],…”

“…Polyphenolic ligands containing multiple hydroxyl groups are increasingly used as linkers for IMAC materials due to their rapid coordination chelation. Various polyphenol chelating agents have been developed, including 2,3,4‐trihydroxybenzaldehyde [112], tannic acid [113], 3,4,5‐trihydroxybenzaldehyde [114], cyclodextrin [115], for the preparation of phosphopeptide‐enriched materials. Recently, with a chelating agent of 2,3,4‐trihydroxybenzaldehyde, Li group [116] proposed a green strategy to fabricate IMAC materials.…”