Design and implementation of software for automated quality control and data analysis for a complex LC/MS/MS assay for urine opiates and metabolites

Dickerson, Jane A.; Schmeling, Michael; Hoofnagle, Andrew N.; Hoffman, Noah G.

doi:10.1016/j.cca.2012.10.055

Cited by 12 publications

(5 citation statements)

References 5 publications

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“…The utility of automated LC-MS/MS data processing and analysis will become even greater with the maturation of automated LC-MS/MS instrumentation given the large sizes of the generated data sets. To enable clinical laboratories to fully harness the valuable information contained within these large-scale data sets, novel fit-for-purpose software tools are needed 91 , 92 . Some laboratories have developed and implemented dashboard tools to monitor, track, optimize and support specialized laboratory workflows that are not typically well supported by routine laboratory information system functionality.…”

Section: Discussionmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometry for clinical diagnostics

Thomas

French

Jannetto

et al. 2022

Nat Rev Methods Primers

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Mass spectrometry is a powerful analytical tool used for the analysis of a wide range of substances and matrices; it is increasingly utilized for clinical applications in laboratory medicine. This Primer includes an overview of basic mass spectrometry concepts, focusing primarily on tandem mass spectrometry. We discuss experimental considerations and quality management, and provide an overview of some key applications in the clinic. Lastly, the Primer discusses significant challenges for implementation of mass spectrometry in clinical laboratories and provides an outlook of where there are emerging clinical applications for this technology.

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Section: Discussionmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometry for clinical diagnostics

Thomas

French

Jannetto

et al. 2022

Nat Rev Methods Primers

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“…The manufacturers and clinical MS community pursued significant progress in regard to regulatory requirements [ 322 , 323 ], standardization of methods [ 324 , 325 ], automation in instrumentation and data analysis [ 326 , 327 ], and flat file interface to laboratory information systems to facilitate seamless order-to-result workflows [ 328 ]. Despite the remarkable promise, one of the major limitations of the clinical MS to date is the identification biomarkers at a very early stage.…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Mass spectrometry-based proteomics as an emerging tool in clinical laboratories

Birhanu

2023

Clin Proteom

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Mass spectrometry (MS)-based proteomics have been increasingly implemented in various disciplines of laboratory medicine to identify and quantify biomolecules in a variety of biological specimens. MS-based proteomics is continuously expanding and widely applied in biomarker discovery for early detection, prognosis and markers for treatment response prediction and monitoring. Furthermore, making these advanced tests more accessible and affordable will have the greatest healthcare benefit.This review article highlights the new paradigms MS-based clinical proteomics has created in microbiology laboratories, cancer research and diagnosis of metabolic disorders. The technique is preferred over conventional methods in disease detection and therapy monitoring for its combined advantages in multiplexing capacity, remarkable analytical specificity and sensitivity and low turnaround time.Despite the achievements in the development and adoption of a number of MS-based clinical proteomics practices, more are expected to undergo transition from bench to bedside in the near future. The review provides insights from early trials and recent progresses (mainly covering literature from the NCBI database) in the application of proteomics in clinical laboratories.

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“…Tools have been introduced to provide data in comprehensive forms, allowing for more facile and higher-quality determinations that reduce the burden on staff [ 112 , 113 ]. Features used in the visual identification of errors from chromatographic illustrations can be metricized and computed quickly and reliably, allowing for review of the flawed data instead of all data.…”

Section: Lc-ms/ms Quality Assurancementioning

confidence: 99%

Review of the Use of Liquid Chromatography-Tandem Mass Spectrometry in Clinical Laboratories: Part II–Operations

Rappold

2022

Ann Lab Med

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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in clinical laboratories because it has advantages in terms of specificity and sensitivity over other analytical technologies. These advantages come with additional responsibilities and challenges given that many assays and platforms are not provided to laboratories as a single kit or device. The skills, staff, and assays used in LC-MS/MS are internally developed by the laboratory, with relatively few exceptions. Hence, a laboratory that deploys LC-MS/MS assays must be conscientious of the practices and procedures adopted to overcome the challenges associated with the technology. This review discusses the post-development landscape of LC-MS/MS assays, including validation, quality assurance, operations, and troubleshooting. The content knowledge of LC-MS/MS users is quite broad and deep and spans multiple scientific fields, including biology, clinical chemistry, chromatography, engineering, and MS. However, there are no formal academic programs or specific literature to train laboratory staff on the fundamentals of LC-MS/MS beyond the reports on method development. Therefore, depending on their experience level, some readers may be familiar with aspects of the laboratory practices described herein, while others may be not. This review endeavors to assemble aspects of LC-MS/MS operations in the clinical laboratory to provide a framework for the thoughtful development and execution of LC-MS/MS applications.

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Design and implementation of software for automated quality control and data analysis for a complex LC/MS/MS assay for urine opiates and metabolites

Cited by 12 publications

References 5 publications

Liquid chromatography–tandem mass spectrometry for clinical diagnostics

Liquid chromatography–tandem mass spectrometry for clinical diagnostics

Mass spectrometry-based proteomics as an emerging tool in clinical laboratories

Review of the Use of Liquid Chromatography-Tandem Mass Spectrometry in Clinical Laboratories: Part II–Operations

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