Design and In Vitro Evaluation of Layer by Layer siRNA Nanovectors Targeting Breast Tumor Initiating Cells

Jaganathan, Hamsa; Mitra, Sucharita; Srinivasan, Srimeenakshi; Dave, Bhuvanesh; Godin, Biana

doi:10.1371/journal.pone.0091986

Cited by 16 publications

(15 citation statements)

References 43 publications

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“…The zeta potential was measured to determine the charge of the RSF solution and the ALG solution over the pH range 2–11 [ 34 ]. The concentration of the RSF and ALG solutions was 1 g/L, and the pH of the solutions was adjusted using hydrochloric acid and sodium hydroxide.…”

Section: Methodsmentioning

confidence: 99%

Surface Modification and Characterisation of Silk Fibroin Fabric Produced by the Layer-by-Layer Self-Assembly of Multilayer Alginate/Regenerated Silk Fibroin

Shen

Guan

et al. 2015

PLoS ONE

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Silk-based medical products have a long history of use as a material for surgical sutures because of their desirable mechanical properties. However, silk fibroin fabric has been reported to be haemolytic when in direct contact with blood. The layer-by-layer self-assembly technique provides a method for surface modification to improve the biocompatibility of silk fibroin fabrics. Regenerated silk fibroin and alginate, which have excellent biocompatibility and low immunogenicity, are outstanding candidates for polyelectrolyte deposition. In this study, silk fabric was degummed and positively charged to create a silk fibroin fabric that could undergo self-assembly. The multilayer self-assembly of the silk fibroin fabric was achieved by alternating the polyelectrolyte deposition of a negatively charged alginate solution (pH = 8) and a positively charged regenerated silk fibroin solution (pH = 2). Finally, the negatively charged regenerated silk fibroin solution (pH = 8) was used to assemble the outermost layer of the fabric so that the surface would be negatively charged. A stable structural transition was induced using 75% ethanol. The thickness and morphology were characterised using atomic force microscopy. The properties of the self-assembled silk fibroin fabric, such as the bursting strength, thermal stability and flushing stability, indicated that the fabric was stable. In addition, the cytocompatibility and haemocompatibility of the self-assembled silk fibroin fabrics were evaluated. The results indicated that the biocompatibility of the self-assembled multilayers was acceptable and that it improved markedly. In particular, after the self-assembly, the fabric was able to prevent platelet adhesion. Furthermore, other non-haemolytic biomaterials can be created through self-assembly of more than 1.5 bilayers, and we propose that self-assembled silk fibroin fabric may be an attractive candidate for anticoagulation applications and for promoting endothelial cell adhesion for vascular prostheses.

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Section: Methodsmentioning

confidence: 99%

Surface Modification and Characterisation of Silk Fibroin Fabric Produced by the Layer-by-Layer Self-Assembly of Multilayer Alginate/Regenerated Silk Fibroin

Shen

Guan

et al. 2015

PLoS ONE

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“…Small interfering RNA (siRNA) is a natural post-transcriptional gene-silencing mechanism to turn off unwanted genes. Targeting STAT3 using siRNA represent a useful approach for the treatment of breast cancer 104 , lung adenocarcinoma 105 . However, further studies should be conducted regarding STAT3 silencing for cancer therapy.…”

Section: Pivotal Biological Functions Of Stat3 In Cancermentioning

confidence: 99%

Multiple regulation pathways and pivotal biological functions of STAT3 in cancer

Yuan

Zhang

Niu

2015

Sci Rep

199

148

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STAT3 is both a transcription activator and an oncogene that is tightly regulated under normal physiological conditions. However, abundant evidence indicates that STAT3 is persistently activated in several cancers, with a crucial position in tumor onset and progression. In addition to its traditional role in cancer cell proliferation, invasion, and migration, STAT3 also promotes cancer through altering gene expression via epigenetic modification, inducing epithelial–mesenchymal transition (EMT) phenotypes in cancer cells, regulating the tumor microenvironment, and promoting cancer stem cells (CSCs) self-renewal and differentiation. STAT3 is regulated not only by the canonical cytokines and growth factors, but also by the G-protein-coupled receptors, cadherin engagement, Toll-like receptors (TLRs), and microRNA (miRNA). Despite the presence of diverse regulators and pivotal biological functions in cancer, no effective therapeutic inventions are available for inhibiting STAT3 and acquiring potent antitumor effects in the clinic. An improved understanding of the complex roles of STAT3 in cancer is required to achieve optimal therapeutic effects.

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“…37 Oligonucleotide decorated AuNPs have been used as intracellular gene regulation agents for controlling the protein expression in cells through conjugation or ionic complexation to various nucleic acids, such as DNA, short hairpin RNA (shRNA), small interfering RNA (siRNA) and microRNA (miRNA). [38][39][40][41] Figure 1 summarizes two common methods to carry various kinds of genes and several release ways. Negatively charged genes can be attached on positively charged AuNPs and cationic linker, peptide, shell conjugated AuNPs.…”

Section: Aunps-based Gene Therapymentioning

confidence: 99%

Gold nanoparticles for cancer theranostics — A brief update

Zhao

Pan

Cheng

et al. 2016

J. Innov. Opt. Health Sci.

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Gold nanoparticles (AuNPs) exhibit superior optical and physical properties for more e®ective treatment of cancer through incorporating both diagnostic and therapeutic functions into one single platform. The ability to passively accumulate on tumor cells provides AuNPs the opportunity to become an attractive contrast agent for X-ray based computed tomography (CT) imaging in vivo. Because of facile surface modi¯cation, various size and shape of AuNPs have been extensively functionalized and applied as active nanoprobes and drug carriers for cancer targeted theranostics. Moreover, their capabilities on producing photoacoustic (PA) signals and photothermal e®ects have been used to image and treat tumor progression, respectively. Herein, we review the developments of AuNPs as cancer diagnostics and chemotherapeutic drug vector, summarizing strategies for tumor targeting and their applications in vitro and in vivo.

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Design and In Vitro Evaluation of Layer by Layer siRNA Nanovectors Targeting Breast Tumor Initiating Cells

Cited by 16 publications

References 43 publications

Surface Modification and Characterisation of Silk Fibroin Fabric Produced by the Layer-by-Layer Self-Assembly of Multilayer Alginate/Regenerated Silk Fibroin

Surface Modification and Characterisation of Silk Fibroin Fabric Produced by the Layer-by-Layer Self-Assembly of Multilayer Alginate/Regenerated Silk Fibroin

Multiple regulation pathways and pivotal biological functions of STAT3 in cancer

Gold nanoparticles for cancer theranostics — A brief update

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