Design and synthesis of a new peptide derived from Fasciola gigantica cathepsin L1 with potential application in serodiagnosis of fascioliasis

Meshgi, B; Jalousian, Fatemeh; Fathi, Saeed; Jahani, Zahra

doi:10.1016/j.exppara.2018.04.013

Cited by 9 publications

(9 citation statements)

References 48 publications

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“…The NPV (94.6%) gives high confidence to identify true negative patients. Higher NPVs than PPVs were also observed previously in peptides derived from cathepsin L1, which exhibited 100% sensitivity for the diagnosis of animal fascioliasis, 11 and 100% sensitivity and 97.3% specificity for the diagnosis of human fascioliasis. 14 By contrast, another putative epitope of CL1 41 demonstrated a similarly low sensitivity (77%) as our peptide, implying that this peptide is not capable of detecting human fascioliasis.…”

Section: Resultssupporting

confidence: 61%

“…[8][9][10] Related to this, recombinant proteins and synthetic peptides based on these molecules have also been used for immunodiagnosis. [11][12][13][14][15] Cathepsin Bs are expressed in the early stages of infection. [16][17][18] For example, F. gigantica cathepsin B5 (FgCB5) has been found in juveniles and in mature adult worms, indicating its potential to detect fascioliasis at the early stage of infection.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit and In-House Fasciola gigantica Cysteine Proteinases-Based Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Fascioliasis

Tran

Intuyod

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Fascioliasis, caused by Fasciola hepatica and Fasciola gigantica infection, is a major food-borne trematodiasis in many places of the world, with the central region of Vietnam being reported as a highly endemic area. Stool examination for Fasciola eggs is not a sensitive method, and immunodiagnostic methods are preferable. We investigated various enzyme-linked immunosorbent assays (ELISAs) to evaluate their efficacy for fascioliasis diagnosis. Test sera used are primarily screened using an ELISA kit produced in Vietnam (VN kit; Viet Sinh Chemical Producing & Trading Co. Ltd., Ho Chi Minh City, Vietnam): Seropositive individuals having symptoms compatible with fascioliasis were regarded as clinically diagnosed fascioliasis cases. A commercial Fasciola IgG ELISA kit from Diagnostic Automation/Cortez Diagnostics, Inc. (USA kit; Woodland Hills, CA), which has been commonly used in Vietnam, was assessed and compared with in-house ELISA systems, including a cystatincapture (CC) ELISA using crude worm extract (CWE) and an indirect ELISA using a synthetic peptide Ac-TPTCHWECQVGYNKTYDEE-NHMe designed from the F. gigantica cathepsin B (FgCB5) molecule. The USA kit was suitable for routine diagnosis after recalibration of the manufacturer's suggested cutoff point. Cystatin-capture ELISA with CWE provided good sensitivity and specificity with perfect agreement to the results of the USA kit. In dotblot ELISA, recombinant FgCB5 reacted more strongly with human antisera than did other F. gigantica antigens tested. Enzyme-linked immunosorbent assay using the synthetic peptide fragment of the FgCB5 exhibited nearly 80% sensitivity and specificity, but the test results showed low agreement with CC-ELISA or the USA kit. In conclusion, the commercially available Fasciola IgG ELISA kit from the United States and the in-house CC ELISA using CWE are suitable for practical diagnosis for fascioliasis.

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Section: Resultssupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit and In-House Fasciola gigantica Cysteine Proteinases-Based Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Fascioliasis

Tran

Intuyod

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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“…Synthetic peptides mimicking relevant B and T cell epitopes are potentially ideal molecules for the development of reagents of diagnostic value (Noya et al 2003), which also represent an interesting perspective to overcome problems observed in vaccine development, for example. Furthermore, studies have been performed using epitope prediction and synthetic peptides that mimic epitopes for diagnosing infectious and parasitic diseases such as strongyloidiasis (Feliciano et al 2016), leishmaniasis (Lage et al 2016), Chagas disease (Elisei et al 2018), and fascioliasis (Meshgi et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Neurocysticercosis serodiagnosis: mimotope-based synthetic peptide as potential biomarker

et al. 2019

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Herein, we evaluate a mimotope-based synthetic peptidenamed NC 4 1 to diagnose neurocysticercosis (NC), a neglected parasitic disease and a major cause of epilepsy worldwide. NC 4 1 synthetic peptide was evaluated to diagnose NC, and total saline extract from Taenia solium metacestodes (SE) was used as control. Serum samples from patients with NC (n = 40), other parasitic diseases (n = 43), and healthy individuals (n = 40) were tested. Diagnostic parameters such as sensitivity (Se), specificity (Sp), likelihood ratio (LR), and area under curve (AUC) were calculated using receiver operating characteristic (ROC) curves. The sequence from T. solium phosphoenolpyruvate carboxykinase (PEPCK) was used for epitope prediction, resulting in one highscoring patch centered at residue L247. NC 4 1 synthetic peptide reached high diagnostic performance (Se 97.5% and Sp 97.5%, LR+ 39 and AUC 0.997). Data from diagnostic parameters and in silico analyses proved the usefulness of NC 4 1 synthetic peptide as a diagnostic marker for human NC.

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“…Though MM3 recognition of endogenous and recombinant CL epitopes has been confirmed, , there is no evidence for MM3-procathepsin L binding activity, thought to be caused by antigen conformational differences . Despite this, the antigenic propensity of the complete CL protein sequence has been mapped, identifying both protease- and zymogen-specific epitopes with immunogenic potential, and as such, peptide derivatives predominantly from the protease region have been tested toward alternative options for fasciolosis diagnosis − and protection . Despite predictions of antigenicity of zymogen oligomers, the abundance and established immunoreactivity of CL protease epitopes with host serum and MM3 has precluded focus on zymogen-specific epitopes for diagnostic consideration.…”

Section: Introductionmentioning

confidence: 99%

Fasciola hepatica Cathepsin L Zymogens: Immuno-Proteomic Evidence for Highly Immunogenic Zymogen-Specific Conformational Epitopes to Support Diagnostics Development

Collett

Phillips

Fisher³

et al. 2022

J. Proteome Res.

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Fasciola hepatica, the common liver fluke and causative agent of zoonotic fasciolosis, impacts on food security with global economic losses of over $3.2 BN per annum through deterioration of animal health, productivity losses, and livestock death and is also re-emerging as a foodborne human disease. Cathepsin proteases present a major vaccine and diagnostic target of the F. hepatica excretory/secretory (ES) proteome, but utilization in diagnostics of the highly antigenic zymogen stage of these proteins is surprisingly yet to be fully exploited. Following an immuno-proteomic investigation of recombinant and native procathepsins ((r)FhpCL1), including mass spectrometric analyses

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Design and synthesis of a new peptide derived from Fasciola gigantica cathepsin L1 with potential application in serodiagnosis of fascioliasis

Cited by 9 publications

References 48 publications

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit and In-House Fasciola gigantica Cysteine Proteinases-Based Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Fascioliasis

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit and In-House Fasciola gigantica Cysteine Proteinases-Based Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Fascioliasis

Neurocysticercosis serodiagnosis: mimotope-based synthetic peptide as potential biomarker

Fasciola hepatica Cathepsin L Zymogens: Immuno-Proteomic Evidence for Highly Immunogenic Zymogen-Specific Conformational Epitopes to Support Diagnostics Development

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