Design and synthesis of DNA-tethered ruthenium complexes that self-assemble into linear arrays

Abstract

Ruthenium(II) bis(terpyridine) complexes have been prepared with two triethylene glycol linkers to which DNA sequences have been attached; hybridization at various complex ratios results in linear arrays of varying lengths.

Metal‐Ion‐Mediated Base Pairs in Nucleic Acids

“…Towards this end, 1-deazaadenine (D) and thymine (T) were chosen as complementary nucleobases. [50] Earlier reports had suggested that the oligonucleotides d(D 20 ) and d(T 20 ) associate via the Hoogsteen edge of the purine base to form a double helix. [123,124] Scheme 7 shows how a metal-ion-mediated base pair is derived from such a Hoogsteen pair by the substitution of a proton by a metal ion.…”

Metal‐Complex/DNA Conjugates: A Versatile Building Block for DNA Nanoarrays

“…The ratio of A:B and B:A mainly dictate the structure of assembly upon hybridisation. For example a ratio of A:B or B:A of 2:1 results in A 2 B or B 2 A trimer as the major component and a longer linear array is formed when the ratio of complexes approaches to 1:1 20. By using a more versatile synthetic strategy, we were able to prepare symmetrical homoleptic metal‐complex/ODN building block (i.e., with one DNA strand on each ligand such as A or B in Figure 4 A).…”

“…Examples of structural diversity of metal-complex/ODN conjugates upon different tethering approaches of ODNs to different metal complexes (taken with permission from references[20][21][22][23][24][25][26][27]). …”

Metal‐Containing Nucleic Acid Structures Based on Synergetic Hydrogen and Coordination Bonding

“…Ni 2þ coordination to cyclam was used only to increase the rigidity of the junction (168). Metal binding to tpy-modified ssDNA led to octahedral [Fe(tpy) 2 ] 2þ complexes (169) or [Ru(tpy) 2 ] 2þ complexes (170), which functioned as two-arm DNA junctions [ Fig. 18(b)].…”

“…18(b)] (169). Hybridization by slow cooling of 1:1 mixtures of two-arm DNA junctions based on bpy-Ru 2þ or tpy-Ru 2þ complexes that had intramolecularly identical but intermolecularly complementary DNA arms led to infinite, linear DNA polymer formation (170,172). In contrast, room temperature hybridization of the same two-arm DNA junctions based on bpy-Ru 2þ led to the formation of a mixture of structures, the majority of which were dimeric and cyclic [ Fig.…”