2017
DOI: 10.1002/bio.3365
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Design and synthesis of the BODIPY–BSA complex for biological applications

Abstract: A quinoxaline-functionalized styryl-BODIPY derivative (S1) was synthesized by microwaveassisted Knoevenagel condensation. It exhibited fluorescence enhancement upon microencapsulation into the hydrophobic cavity of bovine serum albumin (BSA). The S1-BSA complex was characterized systematically using ultraviolet (UV)-visible absorption, fluorescence emission, kinetics, circular dichroism and time-resolved lifetime measurements. The binding nature of BSA towards S1 was strong, and was found to be stable over a p… Show more

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Cited by 13 publications
(7 citation statements)
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“…In fact, similar observations were also noted for other BODIPY dyes reported in the literature. 39 The calculated average lifetime is found to be about 2.5 ns and the magnitude of the total non-radiative decay rate constant, ∑ k nr (0.37 ns −1 ), is found to be much higher than that of the radiative decay rate constant ( k r = 0.01 ns −1 ). The bi-exponential model of 5i fluorescence decay data remains invariant with an increase in the protein concentration (ESI, Table S8†).…”
Section: Resultsmentioning
confidence: 89%
See 1 more Smart Citation
“…In fact, similar observations were also noted for other BODIPY dyes reported in the literature. 39 The calculated average lifetime is found to be about 2.5 ns and the magnitude of the total non-radiative decay rate constant, ∑ k nr (0.37 ns −1 ), is found to be much higher than that of the radiative decay rate constant ( k r = 0.01 ns −1 ). The bi-exponential model of 5i fluorescence decay data remains invariant with an increase in the protein concentration (ESI, Table S8†).…”
Section: Resultsmentioning
confidence: 89%
“…38 Therefore, the study of its interaction with the BODIPY dye can provide us with significant information on the drug design, target identification, drug delivery, mode of interaction and intensity of the pharmacological effect. 39 For using as a possible serum protein sensor, the absorption and fluorescence emission spectra of 5i were analysed in aqueous buffer at pH = 7.4. The absorption peak appeared at 537 nm and the corresponding fluorescence emission peak appeared at 576 nm.…”
Section: Resultsmentioning
confidence: 99%
“…Serum albumins play an important role in maintaining various physiological functions such as the colloid osmotic of blood pressure and drug deposition and efficacy, as it can transport and disposition of endogenous and exogenous compounds present in blood . The nature and magnitude of drug–protein interaction will significantly affect the biological activity such as distribution volume and the clearance rate of the drugs in blood plasma . So, it is of important to understand the interaction of a bioactive compound with protein.…”
Section: Resultsmentioning
confidence: 99%
“…Boron‐dipyrromethene (BODIPY) class of compounds was first described in 1968 [1] . Since then, BODIPY dyes have become one of the most popular fluorophores and are used as emissive dyes, labels, probes, and sensors for diverse applications [2–11] . The reason is strongly absorbing small molecules with sharp absorption and emission bands, and high fluorescence quantum yields.…”
Section: Introductionmentioning
confidence: 99%
“…[1] Since then, BODIPY dyes have become one of the most popular fluorophores and are used as emissive dyes, labels, probes, and sensors for diverse applications. [2][3][4][5][6][7][8][9][10][11] The reason is strongly absorbing small molecules with sharp absorption and emission bands, and high fluorescence quantum yields. They are reasonably stable at physiological conditions.…”
Section: Introductionmentioning
confidence: 99%