Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars

Drábek, Jir̆ı́; Smolíková, Michaela; Kalendar, Ruslan; Pinto, Fernando A Lopes; Pavloušek, Pavel; Klepárnı́k, Karel; Frébort, Ivo

doi:10.1002/elps.201600068

Cited by 3 publications

(2 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hegi) vines (Arroyo-Garcia et al 2006), subsequent crosses between domesticated and local wild vines, the old practice of growing seedlings from spontaneous crosses and conventional breeding. The crosses are attested by the pedigree reconstitution of several varieties such as Cabernet-Sauvignon, which is a progeny of Cabernet franc and Sauvignon blanc (Bowers and Meredith 1997), and Chardonnay and Gamay, which are progenies of Pinot and Gouais (Bowers 1999;Lacombe et al 2013;Myles et al 2011;Cipriani et al 2010;Drabek et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The advent of molecular markers has offered a powerful tool to address these issues; these markers have frequently been used by ampelographers and grapevine geneticists (Cipriani et al 2010;Drabek et al 2016;De Lorenzis et al 2015). Among the many classes of molecular markers proposed in the last 20 years, a relatively new universal retrotransposon-based marker system for DNA fingerprinting, inter-primer binding sites (iPBS), was used by Kalendar et al (Kalendar and Schulman 2014;Kalendar et al 2010Kalendar et al , 2019.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic analysis of the grapevine genotypes of the Russian Vitis ampelographic collection using iPBS markers

et al. 2019

Self Cite

View full text Add to dashboard Cite

Cultivated grapevine (Vitis vinifera L. ssp. sativa D.C.) is one of the oldest agricultural crops, each variety comprising an array of clones obtained by vegetative propagation from a selected vine grown from a single seedling. Most clones within a variety are identical, but some show a different form of accession, giving rise to new divergent phenotypes. Understanding the associations among the genotypes within a variety is crucial to efficient management and effective grapevine improvement. Inter-primer binding-site (iPBS) markers may aid in determining the new clones inside closely related genotypes. Following this idea, iPBS markers were used to assess the genetic variation of 33 grapevine genotypes collected from Russia. We used molecular markers to identify the differences among and within five grapevine clonal populations and analysed the variation, using clustering and statistical approaches. Four of a total of 30 PBS primers were selected, based on amplification efficiency. Polymerase chain reaction (PCR) with PBS primers resulted in a total of 1412 bands ranging from 300 to 6000 bp, with a polymorphism ratio of 44%, ranging from 58 to 75 bands per group. In total, were identified seven private bands in 33 genotypes. Results of molecular variance analysis showed that 40% of the total variation was observed within groups and only 60% between groups. Cluster analysis clearly showed that grapevine genotypes are highly divergent and possess abundant genetic diversities. The iPBS PCR-based genome fingerprinting technology used in this study effectively differentiated genotypes into five grapevine groups and indicated that iPBS markers are useful tools for clonal selection. The number of differences between clones was sufficient to identify them as separate clones of studied varieties containing unique mutations. Our previous phenotypic and phenological studies have confirmed that these genotypes differ from those of maternal plants. This work emphasized the need for a better understanding of the genotypic differences among closely related varieties of grapevine and has implications for the management of its selection processes.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic analysis of the grapevine genotypes of the Russian Vitis ampelographic collection using iPBS markers

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

Isolation and Purification of DNA from Complicated Biological Samples

Kalendar

Boronnikova

Seppänen

2020

Methods in Molecular Biology

View full text Add to dashboard Cite

Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties

et al. 2020

View full text Add to dashboard Cite

The aim of this study was to estimate the genetic diversity of Greek and Bulgarian grapevine varieties with the use of microsatellite markers. The studied samples were collected from various productive vineyards, consisting of eight Greek and nine Bulgarian native varieties. In order to create a genetic profile for each sample, a multiplex PCR reaction method was used amplifying simultaneously seven microsatellite loci. Statistical analysis of data showed that there was a high degree of genetic heterogeneity among most of the varieties studied, highlighting the discriminative power of the chosen set of markers. Moreover, the synonymy of (I) Greek Pamid and Bulgarian Pamid and (II) Greek Zoumiatiko and Bulgarian Dimyat was suggested, as each variety pair had identical allele profiles in all loci examined. Regarding the Greek Mavrud and Bulgarian Mavrud varieties, there was a close genetic relationship between them, however, they did not share common alleles in all microsatellite loci and, therefore, should not be characterized as synonyms. On the other hand, Greek and Bulgarian Keratsouda, which were supposed to be common varieties, were found to be genetically different, supporting that these two varieties should be considered as homonyms. Despite the genotypic assay performed herein, we believe that additional molecular work is needed for the efficient management of Greek and Bulgarian grapevine genepools, as well as to safely suggest any synonym or homonym annotation.

show abstract

Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars

Cited by 3 publications

References 66 publications

Genetic analysis of the grapevine genotypes of the Russian Vitis ampelographic collection using iPBS markers

Genetic analysis of the grapevine genotypes of the Russian Vitis ampelographic collection using iPBS markers

Isolation and Purification of DNA from Complicated Biological Samples

Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties

Contact Info

Product

Resources

About