Abstract-This paper present the investigation on animal's waste treatment by using biodigester. Previously, biodigester has been used widely around the world as an alternative disposal method beside landfill that is more environmental friendly. In the awareness of environmental issue such as leachate pollution, the biodigester is capable to improve surface water and increase water resources quality. The objective of the study is to identify the effeciency of the treatment method for animal's waste using biodigester. In this study, animal's waste were collected from Zoo Negara Malaysia as sources to the biodigester. It is consisted of selected animal's waste from elephants, giraffe, zebra, rhinoceros and etc. The waste was manually screened to remove the course contaminants before be grinded to form a stable paste. Then, the stable paste were diluted and pumped into biodigester tank to be treated in few days and resulting better effluent discharged. The inffluent that had been treated through biodigester had significantly decreased the water quality parameter such as biological oxygen demands (BOD), chemical oxygen demands (COD), ammoniacal nitrogen (NH3-N) and total suspended solid (TSS) in range from 32% to 81%. This had identified that wastewater treatment from animal's waste wastewater could possible using biodigester technology. The biodigester had been proven that the method could treat the animal's inffluent efficiently for a better water quality result, competing the conventional wastewater treatment plant in reducing operational area and cost. Further study could be done for any type of animal's waste at zoo in future as an added value in order to save environment.