Design of a compressed air modulator to be used in comprehensive multidimensional gas chromatography and its application in the determination of pesticide residues in grapes

Pizzutti, Ionara Regina; Vreuls, René J. J.; Kok, Albert; Roehrs, Rafael; Martel, Samile; Friggi, Caroline A.; Zanella, Renato

doi:10.1016/j.chroma.2009.01.088

Cited by 23 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following our previous report (24), air samples in different workplaces, including the office area, workshop, operational area, and filling area, were collected by use of a CD-1 air sampler with fibrous filter membranes (Beijing Detection Instrument Factory, Beijing, China) for 3 consecutive days. Then, FEN was extracted by hexane solvent, and the concentrations were measured by gas chromatography (GC-14B; Shimadzu Co., Kyoto, Japan) (25). The study was approved by the Institutional Review Board of Nanjing Medical University (IRB00001934), and all of the participants signed an informed consent before participating in the study.…”

Section: A Cross-sectional Study Of Fen Factory Workersmentioning

confidence: 99%

Fenvalerate induces oxidative hepatic lesions through an overload of intracellular calcium triggered by the ERK/IKK/NF‐κB pathway

et al. 2018

View full text Add to dashboard Cite

Fenvalerate (FEN), a mainstream pyrethroid pesticide, was initially recommended as a low‐toxicity agent for controlling agricultural and domestic pests. Despite the widespread use of FEN worldwide, little data are available on FEN‐induced hepatic lesions and molecular mechanisms. In the present study, we first performed an occupational cross‐sectional study on FEN factory workers and found that the levels of serum alanine aminotransferase (ALT) and total antioxidant capacity increased, whereas malondialdehyde decreased in laborers in the working areas where the levels of airborne FEN were much higher compared with the office area. The results were then confirmed by animal experiments that abnormal hepatic histology, increased ALT level, and compromised hepatic oxidative capability were observed in rats exposed to a high concentration of FEN. Furthermore, the bioinformatics analysis of gene microarray in rat liver tissue showed that FEN significantly changed the expressions of genes related to the regulation of intracellular calcium ion homeostasis and the calcium signal pathway. Finally, the functional experiments in Buffalo rat liver (BRL) cells demonstrated that FEN first activated ERK MAPK, followed by IKK and NF‐κB, which triggered the transcription of genes responsible for accelerating an overload of intracellular calcium ions, prompted reactive oxygen species generation in the mitochondria, and finally, induced hepatic cellular apoptosis. The calcium signaling pathway and in particular, an overload of intracellular calcium play a critical role in this pathophysiological process via the ERK/IKK/NF‐κB pathway. Our study furthers the understanding of the mechanism of FEN‐induced hepatic injuries and may have implications in the prevention and control of liver diseases induced by environmental pesticides.—Qiu, L.‐L., Wang, C., Yao, S., Li, N., Hu, Y., Yu, Y., Xia, R., Zhu, J., Ji, M., Zhang, Z., Wang, S.‐L. Fenvalerate induces oxidative hepatic lesions through an overload of intracellular calcium triggered by the ERK/IKK/NF‐κB pathway. FASEB J. 33, 2782–2795 (2019). http://www.fasebj.org

show abstract

Section: A Cross-sectional Study Of Fen Factory Workersmentioning

confidence: 99%

Fenvalerate induces oxidative hepatic lesions through an overload of intracellular calcium triggered by the ERK/IKK/NF‐κB pathway

et al. 2018

View full text Add to dashboard Cite

show abstract

“…36 Estes resultados confirmam o que é reportado na literatura, visto que a maioria dos trabalhos publicados apresenta a geometria convencional de colunas (fase estacionária apolar na 1 D e polar na 2 D) como sendo a que proporciona melhores resultados de análise para agrotóxicos em diversas matrizes. 38,59 Processo de extração por ultrassom O processo de extração por ultrassom foi baseado no método reportado por You e colaboradores.…”

Section: Condições Cromatográficasunclassified

“…Existem poucos trabalhos na literatura que trazem resultados quantitativos para determinação de agrotóxicos por GC × GC. Pizzutti e colaboradores 38 desenvolveram um modulador a ar comprimido e avaliaram o seu potencial através da determinação de 6 agrotóxicos da classe dos piretroides em uva e padrões de n-alcanos. Os LOD e LOQ instrumentais para os agrotóxicos ficaram na faixa de 0,003 a 0,006 e de 0,01 a 0,02 mg L -1 , respectivamente.…”

Section: Validação Do Métodounclassified

Aplicação da cromatografia gasosa bidimensional abrangente com microdetector de captura de elétrons para determinação de agrotóxicos em sedimentos

Silva¹,

Zini²,

Caramão³

2011

Quím. Nova

View full text Add to dashboard Cite

. Analysis of seven pesticides in sediments was successfully achieved using comprehensive two-dimensional gas chromatography with micro-electron capture detection, as it provided higher sensitivity and less matrix interference. Repeatability and intermediate precision of peak areas and heights were less than 4% and the recovery percentage for the analytes ranged from 52 to 115%. Instrumental LOD and LOQ were in the range of 0.60 to 2.31 mg L -1 and 1.83 to 5.62 mg L -1, respectively. Concentrations of 3.34 mg kg -1 (dry basis) for trifloxystrobin and azoxystrobin (below the LOQ) were found in a sediment sample.Keywords: pesticides; two-dimensional gas chromatography; micro-electron capture detection. INTRODUÇÃOA utilização de agrotóxicos nas produções agrícolas vem obtendo cada vez mais destaque no cenário mundial. O controle de pragas, através do uso de diferentes princípios ativos, reduziu o índice de doenças para os homens e animais e proporcionou aumento na produção agrícola. Contudo, resíduos desses compostos podem permanecer no ambiente por longos períodos e causar impactos nocivos a diferentes ecossistemas. Nesse contexto, o monitoramento desses compostos em diferentes matrizes como ar, água, solo, sedimento e alimentos se torna de extrema importância para que problemas de saúde pública sejam minimizados e/ou evitados. 1O estudo da ocorrência de agrotóxicos em sedimentos fornece informação a respeito da contaminação do corpo hídrico receptor. O sedimento se comporta como fonte de acumulação de poluentes (compostos orgânicos em geral e metais, por exemplo) e este comportamento se relaciona diretamente com sua constituição físico-química. Este fato confere ao sedimento grande importância no que tange à investigação de processos físicos, geoquímicos e biológicos no ambiente.2 As suas propriedades físico-químicas podem influenciar tanto no transporte e destino desses compostos no ambiente quanto no comportamento dos mesmos durante as etapas de extração, limpeza (clean up) e análise instrumental. A interação existente entre os agrotóxicos e o sedimento pode ser bastante forte, resultando em resíduos quimicamente ligados à matriz. Tendo em vista este contexto, esforços significativos vêm sendo realizados para o desenvolvimento de métodos eficientes que possibilitem a análise multirresíduo de agrotóxicos, minimizando etapas de extração e de clean up das amostras. 24 O processo de extração por ultrassom proporciona um contato mais efetivo entre a matriz e o solvente, devido aos processos térmicos, mecânicos e de cavitação acústica. A exposição à irradiação ultrassônica faz com que haja formação de microbolhas, quando a pressão negativa exercida no sistema é alta o suficiente; sendo que, durante períodos de pressão positiva, as bolhas são comprimidas. Quando ocorre um colapso perto da superfície de um sólido, causado pela compressão e expansão das bolhas, as paredes do sólido podem ser danificadas e ter seu conteúdo (analitos) liberado para o meio. 25 Atualmente, a cromatografia gasosa é a técnica mais utilizada p...

show abstract

“…For the monitoring purposes quick and reliable multiresidual methods which enable simultaneous determination of a wide spectrum of active substances are needed. Extraction is performed by ethylacetate (Berrada et al, 2006;Čajka and Hajšlová, 2004;Ferrer et al, 2005;Sharif et al, 2006), acetonitrile (method also known as QuEChERS method) (Lehotay, 2007;Maštovská et al, 2005) or acetone (Díez et al, 2006;Pizzutti et al, 2009;Stan and Linkerhägner, 1996). We used the multiresidual method in which extraction was performed with acetone, petroleumether and dichlorometane and determination with gas chromatograph coupled to mass spectrometer (GC-MS) (Baša Česnik and Gregorčič, 2003;.…”

Section: Introductionmentioning

confidence: 99%

Pesticide residues in samples of apples, lettuce and potatoes from integrated pest management in Slovenia from 2005-2009

Česnik¹,

Bolta²,

Gregorčič³

2012

Acta Agriculturae Slovenica

View full text Add to dashboard Cite

In the period from 2005 to 2009 225 samples of apples, lettuce and potatoes from Slovene producers included in integrated pest management (IPM) were analysed for plant protection product (PPP) residues. The samples were analysed for the presence of more than 200 different active compounds using four analytical methods. In 38.7% of apple samples residues were not detected, 58.6% of apple samples contained residues lower than or equal to Maximum Residue Levels (MRLs) while 2.7% of apple samples exceeded MRLs. In 84.6% of lettuce samples residues were not detected, 12.3% of lettuce samples contained residues lower than or equal to MRLs while 3.1% of lettuce samples exceeded MRLs. In 98.0% of potato samples residues were not detected, 2.0% of potato samples contained residues lower than or equal to MRLs and no potato samples exceeded MRLs. Multiple residues were found only in apples and lettuce. The trend observed during the years was the decrease of sample portion of samples containing multiple residues in apples from 2005 to 2008. The most frequently found active substance in apples and lettuce was dithiocarbamates. In potato only phosalone was found.

show abstract

Design of a compressed air modulator to be used in comprehensive multidimensional gas chromatography and its application in the determination of pesticide residues in grapes

Cited by 23 publications

References 17 publications

Fenvalerate induces oxidative hepatic lesions through an overload of intracellular calcium triggered by the ERK/IKK/NF‐κB pathway

Fenvalerate induces oxidative hepatic lesions through an overload of intracellular calcium triggered by the ERK/IKK/NF‐κB pathway

Aplicação da cromatografia gasosa bidimensional abrangente com microdetector de captura de elétrons para determinação de agrotóxicos em sedimentos

Pesticide residues in samples of apples, lettuce and potatoes from integrated pest management in Slovenia from 2005-2009

Contact Info

Product

Resources

About