In this paper, a predictor‐based continuous‐discrete nonlinear observer is proposed for a class of nonaffine Lipschitz nonlinear systems with aperiodic sampled delayed output measurements and external disturbance. Firstly, this study introduces a class of delayed sampling hybrid nonlinear systems. By employing Lyapunov techniques and trajectory‐based stability theory, sufficient conditions are established for ensuring input‐to‐state stability of the delayed sampling hybrid nonlinear system with respect to external disturbances. Then the predictor‐based continuous‐discrete nonlinear observer is designed which consists of a continuous‐time observer, a compensating injector and a predictor. The continuous‐time observer is utilized to obtain continuous and delay‐free state estimation. The compensating injector is designed to compensate for output errors that occur between sampling instants. Furthermore, the predictor is employed to obtain delay‐free output error information, which is then utilized by the continuous‐time observer for feedback correction. The proposed observer's exponential input‐to‐state property against the disturbance is proved via the proposed hybrid system stability theory. The effectiveness of the proposed observers has been demonstrated through numerical simulations and performance comparisons with the zero‐order holder‐based observer and the output predictor‐based observer designs in order to highlight the effectiveness and advantages of the proposed observer.