Design of an electrochemiluminescence detection system through the regulation of charge density in a microchannel

Abstract

Rare electrochemiluminescence (ECL) sensors have been developed based on the direct regulation of ionic current because it was difficult to establish a relationship between ionic current and ECL reporting. Ionic...

Electrochemiluminescence Aptasensor for Charged Targets through the Direct Regulation of Charge Density in Microchannels

“…The sensing platform was constructed in our previously work. 26 The three-electrode system, containing GCE, Pt, and Ag/AgCl electrode, was used to excite the luminophore to emit photons under the driving of an external electrical field. GCE and Ag/AgCl were placed in one chamber, and Pt was in another chamber; two separate chambers were linked by microchannels.…”

“…The microchannels were prepared as reported previously . The quartz glass capillaries were cleaned by freshly prepared piranha solution, and then the quartz glasses capillaries were pulled by a micropipette puller under controlled parameters; the diameter of each microchannel was checked by optical microscopy.…”

“…Ion enrichment/depletion in the tip was related to the interface charge density regulated by target recognition reaction, causing the change of ionic conductance and ECL intensity of the system. 26 The sensing principle provided a new idea for the detection of target that can directly or indirectly alter the charge density of microchannels. However, a few sensors have been designed on the basis of the direct regulation of interface charge density by the charged target.…”

“…The microchannels were prepared as reported previously. 26 The quartz glass capillaries were cleaned by freshly prepared piranha solution, and then the quartz glasses capillaries were pulled by a micropipette puller under controlled parameters; the diameter of each microchannel was checked by optical microscopy. The diameter of the microchannel was a key factor for ion transport; the effect of the charge density of microchannels with larger diameters on ion transport became weak; and the microchannel with 3 μm in outer diameter was selected in this work.…”

“…Ion transport in an artificial channel has attracted extensive attention in both fundamental and applied studies. − Previous attempts have revealed that the ionic transport in the micro/nanochannel can be regulated by surface charge density, which can bring opportunities for targets detection, such as ions, molecules, and proteins. − Recently, we combined microchannel-based electrical sensing with ECL technology to design a novel microchannel-based ECL sensing platform for the first time. Ion enrichment/depletion in the tip was related to the interface charge density regulated by target recognition reaction, causing the change of ionic conductance and ECL intensity of the system . The sensing principle provided a new idea for the detection of target that can directly or indirectly alter the charge density of microchannels.…”