Design of Artificial Red Blood Cells using Polymeric Hydrogel Microcapsules: Hydrogel Stability Improvement and Polymer Selection

Zhang, Wujie; Bissen, Matthew J.; Savela, Emily S.; Clausen, Joshua N.; Fredricks, Samantha J.; Guo, Xiaoru; Paquin, Zachary R.; Dohn, Ryan P.; Pavelich, Ian; Polovchak, Alec L.; Wedemeyer, Michael J.; Shilling, Brock E.; Dufner, Emily N.; O’Donnell, Anna C.; Rubio, Gerardo; Readnour, Logan R.; Brown, Tyler; Lee, Jung C.; Kaltchev, M.; Chen, Junhong; Tritt, Charles

doi:10.5301/ijao.5000532

Cited by 10 publications

(16 citation statements)

References 30 publications

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“…The conductivity of LM pectin solutions was found to increase (from 2.68 to 4.79 mS/cm) with increasing pectin concentration (from 3 to 6%). The conductivity of pectin solutions can be attributed to the ionized (carboxyl) groups of the pectin molecules in the solution . Thus, as the concentration of pectin increases, one would expect an increase in the conductivity due to the increase in the number of ionizable carboxyl groups.…”

Section: Resultsmentioning

confidence: 99%

Role of rheology on the formation of Nanofibers from pectin and polyethylene oxide blends

Balik

Argın

2019

J of Applied Polymer Sci

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Characterizing the parameters critical to the prediction of a successful electrospinning process from solution properties is essential, yet, not easy in the case of biopolymers. In this study, we attempted to present a broad perspective by simultaneously evaluating the effect of eight different parameters, namely zero shear viscosity, tip viscosity, elastic modulus, phase angle, cohesive energy, pH, conductivity, and surface tension, on the formation of smooth pectin nanofibers. Our results showed that (1) the viscosity of the solution is not an indication of jet formation, but once the jet is formed, high zero shear viscosity and high tip viscosity are required to maintain a dominant whipping instability for smooth nanofiber formation and (2) while evaluating the elasticity of the spinning solutions, comparing only the elastic modulus values would be misleading, since low phase angle values are also necessary for pectin solutions to be electrospun into nonbeaded fibers. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48294.

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Section: Resultsmentioning

confidence: 99%

Role of rheology on the formation of Nanofibers from pectin and polyethylene oxide blends

Balik

Argın

2019

J of Applied Polymer Sci

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“…The interaction of Ca 2+ and oligochitosan with the pectinbased nanofibers can be observed using Fourier transform infrared (FTIR) spectroscopy (Crouse et al 2015;2016a). As shown in Fig.…”

Section: Chemistry and Surface Charge Of Pectin-based Nanofibersmentioning

confidence: 99%

“…Due to its biocompatibility and biodegradability, pectin has been used for various biological applications: including cell encapsulation, creating artificial red blood cells, bioprinting, and drug delivery (Banks et al 2016;Crouse et al 2015;Harvestine et al 2014;Zhang et al 2016a;Zhang et al 2016b). Very recently, pectin has been explored for fabricating nanofibers.…”

Section: Introductionmentioning

confidence: 99%

“…These cations also have tendencies to leak out of the target material, leading to loss of stability and functionality. In our previous studies, oligochitosan, a natural cationic oligosaccharide, was proven to be an effective nonionic cross-linker of pectin (Crouse et al 2015;Harvestine et al 2014;Zhang et al 2016a;Zhang et al 2016b). Oligochitosan and pectin have complementary charges leading to the formation of polyelectrolytes.…”

Section: Introductionmentioning

confidence: 99%

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Electrospinning pectin-based nanofibers: a parametric and cross-linker study

et al. 2018

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Pectin, a natural biopolymer mainly derived from citrus fruits and apple peels, shows excellent biodegradable and biocompatible properties. This study investigated the electrospinning of pectin-based nanofibers. The parameters, pectin:PEO (polyethylene oxide) ratio, surfactant concentration, voltage, and flow rate, were studied to optimize the electrospinning process for generating the pectin-based nanofibers. Oligochitosan, as a novel and nonionic cross-liker of pectin, was also researched. Nanofibers were characterized by using AFM, SEM, and FTIR spectroscopy. The results showed that oligochitosan was preferred over Ca 2+ because it cross-linked pectin molecules without negatively affecting the nanofiber morphology. Moreover, oligochitosan treatment produced a positive surface charge of nanofibers, determined by zeta potential measurement, which is desired for tissue engineering applications.

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“…Different hemoglobin (Hb)-based oxygen therapeutics have been developed, but, unfortunately, no such product has been approved by the FDA for human use due to the toxicity of free hemoglobin, which can cause hypertension and cardiovascular dysfunction [3,4,5,6]. Nanoscale artificial oxygen transporter carriers, such as nanoparticles and liposomes, have been developed, showing promise in therapies such as wound healing and cancer treatment [7,8].…”

Section: Introductionmentioning

confidence: 99%

Design of a Novel Oxygen Therapeutic Using Polymeric Hydrogel Microcapsules Mimicking Red Blood Cells

et al. 2019

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The goal of this research was to develop a novel oxygen therapeutic made from a pectin-based hydrogel microcapsule carrier mimicking red blood cells. The study focused on three main criteria for developing the oxygen therapeutic to mimic red blood cells: size (5–10 μm), morphology (biconcave shape), and functionality (encapsulation of oxygen carriers; e.g., hemoglobin (Hb)). The hydrogel carriers were generated via the electrospraying of the pectin-based solution into an oligochitosan crosslinking solution using an electrospinning setup. The pectin-based solution was investigated first to develop the simplest possible formulation for electrospray. Then, Design-Expert® software was used to optimize the production process of the hydrogel microcapsules. The optimal parameters were obtained through the analysis of a total of 17 trials and the microcapsule with the desired morphology and size was successfully prepared under the optimized condition. Fourier transform infrared spectroscopy (FTIR) was used to analyze the chemistry of the microcapsules. Moreover, the encapsulation of Hb into the microcapsule did not adversely affect the microcapsule preparation process, and the encapsulation efficiency was high (99.99%). The produced hydrogel microcapsule system shows great promise for creating a novel oxygen therapeutic.

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Design of Artificial Red Blood Cells using Polymeric Hydrogel Microcapsules: Hydrogel Stability Improvement and Polymer Selection

Cited by 10 publications

References 30 publications

Role of rheology on the formation of Nanofibers from pectin and polyethylene oxide blends

Role of rheology on the formation of Nanofibers from pectin and polyethylene oxide blends

Electrospinning pectin-based nanofibers: a parametric and cross-linker study

Design of a Novel Oxygen Therapeutic Using Polymeric Hydrogel Microcapsules Mimicking Red Blood Cells

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