Design of EQCM-MIP sensing matrix for highly specific and sensitive detection of thyroglobulin

Srivastava, J.P.; Singh, Roop Shikha; Singh, Meenakshi

doi:10.1016/j.biosx.2022.100154

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…Another type of interaction is based on adsorbate binding, which is responsible for mass loading and interfacial properties, namely roughness and viscoelasticity. They are observed in the case of the piezoelectric response of microgravimetric immunosensors, wherein the shift in the oscillation frequency is studied [67]. They have attributes of real-time analysis, simplicity, ease of usage, use in both gas and solution phase systems, reliability, and reproducibility.…”

Section: Functional Model Of Bonding Between the Protein And The Unde...mentioning

confidence: 99%

“…Their sensitivity is quite enhanced, and one of these systems is named quartz crystal microbalance (QCM). A review has been published in this regard involving epitome-based imprinted sensors for electrochemical-based timely disease monitoring, using EQCM as one of the potential techniques [67]. A recent report accounts for the detection of Salmonella typhimurium in chicken meat by the immobilization of the protein A-based antibody immunosensor [68].…”

Section: Functional Model Of Bonding Between the Protein And The Unde...mentioning

confidence: 99%

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Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Gandhi

2024

Electrochem

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Electrochemistry is a hotspot in today’s research arena. Many different domains have been extended for their role towards the Internet of Things, digital health, personalized nutrition, and/or wellness using electrochemistry. These advances have led to a substantial increase in the power and popularity of electroanalysis and its expansion into new phases and environments. The recent COVID-19 pandemic, which turned our lives upside down, has helped us to understand the need for miniaturized electrochemical diagnostic platforms. It also accelerated the role of mobile and wearable, implantable sensors as telehealth systems. The major principle behind these platforms is the role of electrochemical immunoassays, which help in overshadowing the classical gold standard methods (reverse transcriptase polymerase chain reaction) in terms of accuracy, time, manpower, and, most importantly, economics. Many research groups have endeavoured to use electrochemical and bio-electrochemical tools to overcome the limitations of classical assays (in terms of accuracy, accessibility, portability, and response time). This review mainly focuses on the electrochemical technologies used for immunosensing platforms, their fabrication requirements, mechanistic objectives, electrochemical techniques involved, and their subsequent output signal amplifications using a tagged and non-tagged system. The combination of various techniques (optical spectroscopy, Raman scattering, column chromatography, HPLC, and X-ray diffraction) has enabled the construction of high-performance electrodes. Later in the review, these combinations and their utilization will be explained in terms of their mechanistic platform along with chemical bonding and their role in signal output in the later part of article. Furthermore, the market study in terms of real prototypes will be elaborately discussed.

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Section: Functional Model Of Bonding Between the Protein And The Unde...mentioning

confidence: 99%

Section: Functional Model Of Bonding Between the Protein And The Unde...mentioning

confidence: 99%

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Gandhi

2024

Electrochem

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“…Molecularly imprinted polymers (MIPs) are synthetic receptors that emulate the recognition properties of their target molecules . MIPs are synthesized by performing a polymerization reaction in the presence of target molecules, which serve as templates. , The monomers congregate around the target molecules; consequently, the polymerization reaction produces a structure around the templates . Removing the templates from the polymer matrix leaves recognition cavities complementary to the target molecules in terms of shape and position of the functional bodies .…”

Section: Introductionmentioning

confidence: 99%

“… 16 , 17 The monomers congregate around the target molecules; consequently, the polymerization reaction produces a structure around the templates. 18 Removing the templates from the polymer matrix leaves recognition cavities complementary to the target molecules in terms of shape and position of the functional bodies. 19 Thus, the target molecules can selectively bind to the cavities created in MIPs via a combination of noncovalent interactions.…”

Section: Introductionmentioning

confidence: 99%

Electric Field-Assisted Molecularly Imprinted Polymer-Modified QCM Sensor for Enhanced Detection of Immunoglobulin

LariMojarad,

Mousavi,

Moeini Manesh

et al. 2024

ACS Omega

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In this study, an electric-field-assisted molecularly imprinted polymer (EFAMIP) as an enhanced form of MIP was developed to improve the MIP-modified quartz crystal microbalance (QCM) biosensors. While exerting a vertical electric field, polymerization of methacrylic acid in the presence of immunoglobulin G (IgG) as the template was initiated, and later, after the template removal process, the EFAMIPs were obtained. The polymer surface characterization was conducted by using a scanning electron microscope. The impact of electric field direction on IgG binding sites, forming either EFAMIP-F ab or EFAMIP-F c , was assessed. Next, the static measurement results in liquid for EFAMIP-modified QCM and MIP-modified QCM were compared. While encompassing IgG, EFAMIP-modified QCMs exhibited up to a 113.5% higher frequency shift than typical MIP in time-limited detection. The final frequency shift of EFAMIP, which determines the detection limit of IgG, was improved up to 12.5% compared to typical MIP. Moreover, the EFAMIP-F ab performance was promising for the selective detection of IgG in a solution containing different types of immunoglobulins.

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Highly sensitive detection of thyroglobulin based on sandwich-type electrochemical immunoassay

Zhang

Lin

et al. 2023

ANAL. SCI.

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Design of EQCM-MIP sensing matrix for highly specific and sensitive detection of thyroglobulin

Cited by 4 publications

References 26 publications

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Electric Field-Assisted Molecularly Imprinted Polymer-Modified QCM Sensor for Enhanced Detection of Immunoglobulin

Highly sensitive detection of thyroglobulin based on sandwich-type electrochemical immunoassay

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