Design of Oriented Porous PHBV Scaffold as a Neural Guide

Abstract

The aim of this study was to produce an oriented biodegradable poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nerve guide. Oriented porous micropatterned artificial nerve guide designed onto the micro-patterned silicon wafers, then their surfaces modified with to oxygen plasma for cell attachment increasing. Afterwards, the neural guides were evaluated by microscopic, structural, physical, and mechanical analyses, and cell culture assays with Schwann cells. Results of structural, physical, and mechanical analyses showed a good resilience and compliance with movement as a neural graft. Cellular experiments showed a better cell adhesion, growth, and proliferation inside the plasma-modified neural guides compared to un-modified ones, also Schwann cells were well attached on plasma-modified surface. This neural guide appears to have the right organization for testing in vivo nerve tissue engineering studies.

Electrospun Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate)/Hydroxyapatite Scaffold With Unrestricted Somatic Stem Cells for Bone Regeneration

“…Only several PHAs, including poly 3-hydroxybutyrate (PHB), copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV), poly 4-hydroxybutyrate (P4HB), copolymers of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), and poly 3-hydroxyoctanoate (PHO), are available in sufficient quantity for application research. [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Hydroxyapatite [HAp, Ca 10 (PO 4 ) 6 (OH) 2 ] is a type of calcium phosphates, which has extensive applications in the healing of bone and tooth, because of biocompatibility and similar composition to natural bone. 30,31 In the current study, unrestricted somatic stem cells (USSCs) were used as a regenerative cell for the growth and repairing of bone defect.…”

Enhancement of Fibroblasts Outgrowth onto Polycaprolactone Nanofibrous Grafted by Laminin Protein Using Carbon Dioxide Plasma Treatment

“…Controlling surface properties is so important for the high performance of adhesion [7]. Biomaterials wettability is a necessary factor in the surface modification of materials.…”

Application of polymeric nanofibers in soft tissues regeneration

“…Co‐cultured cells may be useful in management to support wound healing in corneal and scleral ulceration . In vivo transplantation of a nanofibrous scaffold with neural cells into transected rat sciatic nerve (peripheral nervous system) showed accelerated histologic regeneration of the nerve, increased myelinated axons, and improved electrophysiologic function . In a study involving optic nerve injury, a polymeric nanofibrous substrate composed of polyglycolic acid and chitosan was coated with a recombinant neuronal adhesion protein called L1.…”

“…Regenerating axons are accurately guided for long distances along naturally occurring bands of Bungner when the nerve defect is bridged. The most popular approach in peripheral nerve tissue engineering involves in vivo implantation of artificial scaffolds and substrates that will guide naturally regenerating axons to the distal segment …”