Design of Recombinant Antibody Microarrays for Cell Surface Membrane Proteomics

Dexlin, Linda; Ingvarsson, Johan; Frendéus, Björn; Borrebaeck, Carl; Wingren, Christer

doi:10.1021/pr070257x

Cited by 33 publications

(21 citation statements)

References 58 publications

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“…High-performing recombinant antibody microarrays have been developed for immunophenotyping, paving the way for large-scale analysis [19]. Indeed, antibody-based microarray techniques have been used to determine discriminating surface antigen (CD) expression profiles for different B-cell populations and their correlations to discrete leukemia subtypes as well as drug target identification of leukemia cells [20–23].…”

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confidence: 99%

Interfacing Antibody-Based Microarrays and Digital Holography Enables Label-Free Detection for Loss of Cell Volume

et al. 2015

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Background:We introduce the combination of digital holographic microscopy (DHM) and antibody microarrays as a powerful tool to measure morphological changes in specifically antibody-captured cells. The aim of the study was to develop DHM for analysis of cell death of etoposide-treated suspension cells.Results/methodology:We demonstrate that the cell number, mean area, thickness and volume were noninvasively measured by using DHM. The cell number was stable over time, but the two cell lines showed changes of cell area and cell irregularity after treatment. The cell volume in etoposide-treated cells was decreased, whereas untreated cells showed stable volume.Conclusion:Our results provide proof of concept for using DHM combined with antibody-based microarray technology for detecting morphological changes in captured cells.

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confidence: 99%

Interfacing Antibody-Based Microarrays and Digital Holography Enables Label-Free Detection for Loss of Cell Volume

et al. 2015

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“…Numerous recent technologies such as next-generation sequencing (NGS)[85] and microarray technologies[86,87] have adopted in searching for different biomarkers emerged in era of “omics”[88,89]. The progress in high-throughput technologies used in ease way to examine a whole tumor genome (genomics, transcriptomics, proteomics) feature important advances in understanding of the underlying sophisticated pathomechanism for carcinogenesis and metastasis of HCC leading to discover of promising biomarkers with clinical potential.…”

Section: Biomarkers For Hcc Early Detectionmentioning

confidence: 99%

Insights for hepatitis C virus related hepatocellular carcinoma genetic biomarkers: Early diagnosis and therapeutic intervention

Ezzat

Amr

2016

WJH

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The current review explores the role of emerging molecular contributing factors in liver carcinogenesis on top of hepatitis C virus (HCV). Here we will try to discuss the role genetic and epigenetic factors in pathogenesis of hepatocellular carcinoma. Understanding the role of these factors will help in discovering the mystery of liver carcinogenesis on top of chronic HCV infection. Moreover, use of the studied molecular factors will provide the hepatologists with tailored diagnostic promising biomarkers and flatten the way for establishment of emerging molecular treatment based on exploring the molecular subscription of this aggressive liver cancer.

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“…This underscores the need for largescale development of new antibodies to a wider range of proteins. Because traditional monoclonal and polyclonal antibodies are laborious and time-consuming to produce, recombinant antibody phage-display libraries are currently being explored as an alternate source of molecular probes [44][45][46].…”

Section: Biomarker Discovery and Development Platformsmentioning

confidence: 99%

Cytokine Antibody Arrays in Biomarker Discovery and Validation

Huang¹,

Burkholder²,

Jones³

et al. 2012

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Many normal physiological and disease-related pathophysiological processes are regulated by the interactions of complex signaling networks of pro-and anti-inflammatory markers, growth factors, soluble receptors and extracellular matrix proteins, as well as other cell-cell signaling proteins, which we define collectively as cytokines. Because multiple cell-cell signaling factors may be involved in a single biological process, detection of expression of multiple cytokines is essential to unraveling the mechanisms and effects of many disease processes. Cytokine antibody arrays have been developed to meet this growing demand for multiplexed protein detection. In particular, discovery and validation of diseaserelated protein biomarkers require high-throughput detection of many proteins simultaneously. This review will address the complexity of cytokine biology, discuss current and future antibody array technologies and explore their applications in cytokine biomarker discovery and validation for variety of human diseases. Specific examples of these applications will be presented, including the search for cytokine biomarkers related to neurological and neurodegenerative diseases (such as autism and Alzheimer's), immunological disorders (including asthma), and various cancers.

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Design of Recombinant Antibody Microarrays for Cell Surface Membrane Proteomics

Cited by 33 publications

References 58 publications

Interfacing Antibody-Based Microarrays and Digital Holography Enables Label-Free Detection for Loss of Cell Volume

Interfacing Antibody-Based Microarrays and Digital Holography Enables Label-Free Detection for Loss of Cell Volume

Insights for hepatitis C virus related hepatocellular carcinoma genetic biomarkers: Early diagnosis and therapeutic intervention

Cytokine Antibody Arrays in Biomarker Discovery and Validation

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