Design of scaffolds for blood vessel tissue engineering using a multi-layering electrospinning technique

Vaz, CM Claudia; Tuijl, van S Sjoerd; Bouten, Cvc Carlijn; Baaijens, Fpt Frank

doi:10.1016/j.actbio.2005.06.006

“…Multicomponent fibers can be obtained mainly by two techniques [5]: direct electrospinning of polymers solution (in a single-needle configuration, if a mixture of polymers is co-dissolved in the electrospinning solution or a multi-needle configuration in which the polymer solutions are separated in parallel or concentric syringes) and post-treatment of the single-component electrospun fibers (which can include coating with other inorganic-polymer layers [6,7], grafting [8], crosslinking [9], chemical vapour deposition [10], functionalization with other (bio)polymers [11]). In addition to the new physico-chemical properties that arise from using various components, a variety of fiber structures can be obtained such as core-shell fibers, micro/nanotubes, interpenetrating phase morphologies (matrix dispersed or co-continuous fibers) [12,13], nanoscale morphologies (spheres, rods, micelles, lamellae, vesicle tubules, and cylinders) [14] and multilayered constructs (either with different composition or different fiber diameter) [15,16].…”

Section: Introductionmentioning

confidence: 99%

Effects of drug solubility, state and loading on controlled release in bicomponent electrospun fibers

Natu

¹

,

Sousa

²

,

Gil

³

2010

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Bicomponent fibers of two semi-crystalline (co)polymers, poly(ε-caprolactone), PCL and poly(oxyethylene-b-oxypropylene-b-oxyethylene), Lu were obtained by electrospinning. Acetazolamide and timolol maleate were loaded in the fibers in different concentrations (below and above the drug solubility limit in polymer) in order to determine the effect of drug solubility in polymer, drug state, drug loading and fiber composition on fiber morphology, drug distribution and release kinetics. The high loadings fibers (with drug in crystalline form) showed higher burst and faster release than low drug content fibers, indicating the release was more sustained when the drug was encapsulated inside the fibers, in amorphous form. Moreover, timolol maleate was released faster than acetazolamide, indicating that drug solubility in polymer influences the partition of drug between polymer and elution medium, while fiber composition also controlled drug release. At low loadings, total release was not achieved (cumulative release percentages smaller than 100 %), suggesting that drug remained trapped in the fibers. The modeling of release data implied a three stage release mechanism: a dissolution stage, a desorption and subsequent diffusion through water filled pores, followed by polymer degradation control.

show abstract

“…A previous study also utilized electrospinning to fabricate an artificial blood vessel with two separate layers [9]. In this work, the copolymer was sprayed using PCL-gelatin and PLGA-chitosan connected by a middle layer (PLGA-gelatin), as shown in figures 5(b)-(d).…”

Section: Discussionmentioning

confidence: 99%

“…A novel method that involves simultaneous electrospinning of two polymer solutions delivered by two syringe pumps at different flow rates has recently been used to fabricate multicomponent scaffolds [8]. Vaz et al and Kim et al developed bilayer scaffolds for blood vessel applications using PLA/PCL [9] and PLGA [10], respectively. However, the separation of the inner and outer layer in those scaffolds resulted in poor mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

The effect of cross-linking on the microstructure, mechanical properties and biocompatibility of electrospun polycaprolactone–gelatin/PLGA–gelatin/PLGA–chitosan hybrid composite

Nguyen

¹

,

Lee

²

2012

Science and Technology of Advanced Materials

View full text Add to dashboard Cite

In this study, multilayered scaffolds composed of polycaprolactone (PCL)-gelatin/poly(lactic-co-glycolic acid) (PLGA)-gelatin/PLGA-chitosan artificial blood vessels were fabricated using a double-ejection electrospinning system. The mixed fibers from individual materials were observed by scanning electron microscopy. The effects of the cross-linking process on the microstructure, mechanical properties and biocompatibility of the fibers were examined. The tensile stress and liquid strength of the cross-linked artificial blood vessels were 2.3 MPa and 340 mmHg, respectively, and were significantly higher than for the non-cross-linked vessel (2.0 MPa and 120 mmHg). The biocompatibility of the cross-linked artificial blood vessel scaffold was examined using the MTT assay and by evaluating cell attachment and cell proliferation. The cross-linked PCL-gelatin/PLGA-gelatin/ PLGA-chitosan artificial blood vessel scaffold displayed excellent flexibility, was able to withstand high pressures and promoted cell growth; thus, this novel material holds great promise for eventual use in artificial blood vessels.

show abstract

“…Compared to other polyester family members such as PLA, PGA, and PLGA, PCL has been used less frequently as a material for fabricating biomaterial scaffolds, mainly because of concern over its slower degradation kinetics [136]. However, the improved resistance to hydrolytic attack and lower cost than other biodegradable polymers also make PCL an attractive polymer for the fabrication of electrospun nanofibers [53,77,99,100,[137][138][139][140][141][142][143][144][145][146]. The rationale for using a biodegradable polymer with a longer half-life is to provide a structurally stable environment that is able to initiate and promote cell growth for a sufficient period of time.…”

Section: Synthetic Polymeric Nanofibrous Scaffoldsmentioning

confidence: 99%

Electrospinning Technology for Nanofibrous Scaffolds in Tissue Engineering

Li¹,

Shanti²,

Tuan³

2003

Nanotechnologies for the Life Sciences

View full text Add to dashboard Cite

The sections in this article are Introduction Nanofibrous Scaffolds Fabrication Methods for Nanofibrous Scaffolds Phase Separation Self‐assembly Electrospinning The Electrospinning Process History Setup Mechanism and Working Parameters Properties of Electrospun Nanofibrous Scaffolds Architecture Porosity Mechanical Properties Current Development of Electrospun Nanofibrous Scaffolds in Tissue Engineering Evidence Supporting the Use of Nanofibrous Scaffolds in Tissue Engineering Nanofibrous Scaffolds Enhance Adsorption of Cell Adhesion Molecules Nanofibrous Scaffolds Induce Favorable Cell– ECM Interaction Nanofibrous Scaffolds Maintain Cell Phenotype Nanofibrous Scaffolds Support Differentiation of Stem Cells Nanofibrous Scaffolds Promote in vivo ‐like 3 D Matrix Adhesion and Activate Cell Signaling Pathway Biomaterials Electrospun into Nanofibrous Scaffolds Natural Polymeric Nanofibrous Scaffolds Synthetic Polymeric Nanofibrous Scaffolds Composite Polymeric Nanofibrous Scaffolds Nanofibrous Scaffolds Coated with Bioactive Molecules Engineered Tissues using Electrospun Nanofibrous Scaffolds Skin Blood Vessel Cartilage Bone Muscle Ligament Nerve Current Challenges and Future Directions Conclusion

show abstract

Design of scaffolds for blood vessel tissue engineering using a multi-layering electrospinning technique

Cited by 412 publications

References 29 publications

Effects of drug solubility, state and loading on controlled release in bicomponent electrospun fibers

Effects of drug solubility, state and loading on controlled release in bicomponent electrospun fibers

The effect of cross-linking on the microstructure, mechanical properties and biocompatibility of electrospun polycaprolactone–gelatin/PLGA–gelatin/PLGA–chitosan hybrid composite

Electrospinning Technology for Nanofibrous Scaffolds in Tissue Engineering

Contact Info

Product

Resources

About