Design of temozolomide-loaded proliposomes and lipid crystal nanoparticles with industrial feasible approaches: comparative assessment of drug loading, entrapment efficiency, and stability at plasma pH

Waghule, Tejashree; Rapalli, Vamshi Krishna; Singhvi, Gautam; Gorantla, Srividya; Khosa, Archana; Dubey, Sunil Kumar; Saha, Ranendra Narayan

doi:10.1080/08982104.2020.1748648

Cited by 44 publications

(20 citation statements)

References 26 publications

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“…This could be explained by the higher amount of CBE entrapped within vesicles containing a low amount of phospholipid, which increases the time necessary for CBE diffusion from the vesicles to the external environment. As a result, it provides a sustained release pattern (Waghule et al., 2021 ). Moreover, increasing SGC amount (X 3 ) significantly increased RE% ( P = 0.0004 ).…”

Section: Resultsmentioning

confidence: 99%

Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats

et al. 2022

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Cranberry extract (CBE) is a major source of the antioxidant polyphenolics but suffers from limited bioavailability. The goal of this research was to encapsulate the nutraceutical (CBE), into bile salt augmented liposomes (BSALs) as a promising oral delivery system to potentiate its hepatoprotective impact against dimethylnitrosamine (DMN) induced liver injury in rats. The inclusion of bile salt in the liposomal structure can enhance their stability within the gastrointestinal tract and promote CBE permeability. CBE loaded BSALs formulations were fabricated utilizing a (2 3 ) factorial design to explore the impact of phospholipid type (X 1 ), phospholipid amount (X 2 ), and sodium glycocholate (SGC) amount (X 3 ) on BSALs properties, namely; entrapment efficiency percent, (EE%); vesicle size, (VS); polydispersity index; (PDI); zeta potential, (ZP); and release efficiency percent, (RE%). The optimum formulation (F1) exhibited spherical vesicles with EE% of 71.27 ± 0.32%, VS; 148.60 ± 6.46 nm, PDI; 0.38 ± 0.02, ZP; −18.27 ± 0.67 mV and RE%; 61.96 ± 1.07%. Compared to CBE solution, F1 had attenuated DMN-induced hepatic injury, as evidenced by the significant decrease in serum level of ALT, AST, ALP, MDA, and elevation of GSH level, as well as SOD and GPX activities. Furthermore, F1 exhibited an anti-inflammatory character by suppressing TNF-α, MCP-1, and IL-6, as well as downregulation of VEGF-C, STAT-3, and IFN-γ mRNA levels. This study verified that when CBE was integrated into BSALs, F1, its hepatoprotective effect was significantly potentiated to protect the liver against DMN-induced damage. Therefore, F1 could be deliberated as an antioxidant, antiproliferative, and antifibrotic therapy to slow down the progression of hepatic damage.

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Section: Resultsmentioning

confidence: 99%

Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats

et al. 2022

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“…For this reason, reports of TMZ-loaded liposomes generated through thin-film hydration use TMZ dissolved either in the lipid [51,55,82] or in the aqueous phase [52,80]. The reported TMZ-encapsulating MLVs are generally extruded for obtaining a more homogenous population of SUVs, although unextruded LUVs formed by hydrating sieved dry lipid films (proliposomes) have also been reported [9,114], as well as SUVs obtained through sonication of hydrated lyophilized lipid cakes [52].…”

Section: Thin-film Hydration (Thf)mentioning

confidence: 99%

“…TMZ-loaded liposomes have been also generated by hydrating sieved proliposomes obtained through the film deposition on carrier method using mannitol [114] and sorbitol [9] as carriers, but with low EE% (22.68% and 35.45%, respectively). Interestingly, in the case of mannitol-stabilized proliposomes, TMZ was dissolved in the lipid phase, and liposomes were formed through hydration of the sieved proliposome powder with acetate buffer, whereas in the case of sorbitol-stabilized proliposomes, TMZ was dissolved in the hydrating solution.…”

Section: Thin-film Hydration (Thf)mentioning

confidence: 99%

Liposomal-Based Formulations: A Path from Basic Research to Temozolomide Delivery Inside Glioblastoma Tissue

et al. 2022

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Glioblastoma (GBM) is a lethal brain cancer with a very difficult therapeutic approach and ultimately frustrating results. Currently, therapeutic success is mainly limited by the high degree of genetic and phenotypic heterogeneity, the blood brain barrier (BBB), as well as increased drug resistance. Temozolomide (TMZ), a monofunctional alkylating agent, is the first line chemotherapeutic drug for GBM treatment. Yet, the therapeutic efficacy of TMZ suffers from its inability to cross the BBB and very short half-life (~2 h), which requires high doses of this drug for a proper therapeutic effect. Encapsulation in a (nano)carrier is a promising strategy to effectively improve the therapeutic effect of TMZ against GBM. Although research on liposomes as carriers for therapeutic agents is still at an early stage, their integration in GBM treatment has a great potential to advance understanding and treating this disease. In this review, we provide a critical discussion on the preparation methods and physico-chemical properties of liposomes, with a particular emphasis on TMZ-liposomal formulations targeting GBM developed within the last decade. Furthermore, an overview on liposome-based formulations applied to translational oncology and clinical trials formulations in GBM treatment is provided. We emphasize that despite many years of intense research, more careful investigations are still needed to solve the main issues related to the manufacture of reproducible liposomal TMZ formulations for guaranteed translation to the market.

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“…199 The DDSolver is used quite frequently for the evaluation of drug release from nanofibers and in determining release kinetics. 146,[200][201][202] For oral dosage forms produced by nano spray drying, the determination of drug release kinetics and the use of the DDSolver program have been reported to be useful. 175,177,178,188,203,204 In a study with NPs prepared with Eudragit RS, Eudragit RL, and Kollidon SR polymers containing anti-inflammatory DT, the release kinetics conformed to the Korsmeyer-Peppas model.…”

Section: Release Kinetics Of Nano Spray-dried Particlesmentioning

confidence: 99%

Nano Spray-Dried Drugs for Oral Administration: A Review

Öztürk

Arpagaus

2021

ASSAY and Drug Development Technologies

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Spray drying is an important technology that is fast, simple, reproducible, and scalable. It has a wide application range, that is, in food, chemicals, and encapsulation of pharmaceuticals. The technology can be divided into conventional spray drying and nano spray drying. The key advantage of nano spray drying is the production of drug-loaded nanosized particles for various drug delivery applications. The recent developments in nano spray dryer technology and the market launch of the Nano Spray Dryer B-90 by Bu ¨chi Labortechnik AG in 2009 enabled the production of submicron spray-dried particles. This review focuses on nanosized drug delivery systems intended for oral administration produced by nano spray drying. First, the nano spray drying concept, the basic technologies implemented in the equipment, and the effects of the various process parameters on the final dry submicron powder properties are presented. Then, the topics of new formulation strategies of oral drugs are highlighted with examples that have entered the research literature in recent years. Next, the subjects of direct conversion of poorly watersoluble drugs, encapsulation of drugs, and drying of preformed nanoparticles are considered. Finally, topics such as morphology, particle size, size distribution, surface analysis, bioavailability, drug release, release kinetics, and solid-state characterization (by differential scanning calorimetry, X-ray diffraction, Fourier transform infrared spectroscopy, nuclear magnetic resonance) of oral drug delivery systems produced by nano spray drying are discussed. The review attempts to provide a comprehensive knowledge base with current literature and foresight to researchers working in the field of pharmaceutical technology and nanotechnology and especially in the field of nano spray drying.

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Design of temozolomide-loaded proliposomes and lipid crystal nanoparticles with industrial feasible approaches: comparative assessment of drug loading, entrapment efficiency, and stability at plasma pH

Cited by 44 publications

References 26 publications

Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats

Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats

Liposomal-Based Formulations: A Path from Basic Research to Temozolomide Delivery Inside Glioblastoma Tissue

Nano Spray-Dried Drugs for Oral Administration: A Review

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